Medical Radiology and Radiation Safety. 2026. Vol. 71. № 3
DOI:10.33266/1024-6177-2026-71-3-5-10
Yu.B. Deshevoi1, V.G. Lebedev1, T.A. Nasonova1, O.A. Dobrynina1, V.A. Brunchukov1, I.V. Kobzeva1, T.A. Astrelina1, S.V. Lischuk1, E.A. Dubova1, K.A. Pavlov1, O.Ph. Serova2, Yu.D. Udalov1
Treatment of Severe Local Radiation Injuries in Rats by Tissue-Engineered Matrix Application with Syngeneic Adipose Tissue Stromal Vascular Fraction (SVF) Transplantation and Drug Therapy
1 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2 Treatment of Severe Local Radiation Injuries in Rats by Tissue-Engineered Matrix Application with Syngeneic Adipose Tissue Stromal Vascular Fraction (SVF) Transplantation and Drug Therapy
Contact person: Yu.B. Deshevoi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract
Purpose: To investigate the therapeutic efficacy of a tissue-engineered scaffold in combination with stromal vascular fraction (SVF) cell transplantation and the use of medications that improve the trophic status of irradiated tissues, in cases of severe local radiation injuries in experimental animals.
Material and methods: The experiment was performed on male Wistar-Kyoto rats weighing 260–280 g. The animals were locally irradiated in the iliopsoas region of the back using an LNK-268 X-ray unit (RAP 100–10) at a dose of 90 Gy (tube voltage 30 kV, current 6.1 mA, Al filter 0.1 mm thick) with a dose rate of 20.0 Gy/min. The irradiation field area was 8.5 cm². Following radiation exposure, the rats developed long-lasting, non-healing (up to 3–4 months) radiation-induced skin ulcers. During the period of 21–28 days after irradiation, when the radiation ulcer was formed and signs of gradual healing appeared, we initiated treatment for severe local radiation injuries. For this purpose, a tissue-engineered scaffold (decellularized human amnion) was applied to the radiation ulcer, combined with syngeneic SVF cell transplantation and drug therapy. Medications used were drugs acting on microcirculation and the trophic status of irradiated tissues – pentoxifylline and Detralex. The tissue-engineered scaffold was applied to the radiation ulcer 28 days after rat irradiation. Syngeneic SVF cells were transplanted once (2.1 × 10⁶ cells) subcutaneously around the radiation ulcer on day 27 after irradiation (i.e., 1 day before scaffold application). Drug therapy was administered from day 21 to day 42 after irradiation.
Results and conclusion: The obtained data indicate that in the treatment of severe local radiation injuries, the application of a tissue-engineered scaffold in combination with SVF cell transplantation and the use of medications that improve the trophic status of irradiated tissues is more effective compared to the separate use of the scaffold, cell therapy, or drug therapy alone.
Keywords: radiation ulcer, tissue-engineered scaffold, transplantation of cells, adipose tissues, stromal-vascular fraction, drug the-rapy, rats
For citation: Deshevoi YuB, Lebedev VG, Nasonova TA, Dobrynina OA, Brunchukov VA, Kobzeva IV, Astrelina TA, Lischuk SV, Dubova EA, Pavlov KA, Serova OPh, Udalov YuD. Treatment of Severe Local Radiation Injuries in Rats by Tissue-Engineered Matrix Application with Syngeneic Adipose Tissue Stromal Vascular Fraction (SVF) Transplantation and Drug Therapy. Medical Radiology and Radiation Safety. 2026;71(3):5–10. DOI:10.33266/1024-6177-2026-71-3-5-10
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Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.01.2026. Accepted for publication: 25.02.2026.