JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.
Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.
The journal is included in the List of Russian Reviewed Scientific Journals of the Higher Attestation Commission. Since 2008 the journal has been available on the Internet and indexed in the RISC database which is placed on Web of Science. Since February 2nd, 2018, the journal "Medical Radiology and Radiation Safety" has been indexed in the SCOPUS abstract and citation database.
Brief electronic versions of the Journal have been publicly available since 2005 on the website of the Medical Radiology and Radiation Safety Journal: http://www.medradiol.ru. Since 2011, all issues of the journal as a whole are publicly available, and since 2016 - full-text versions of scientific articles. Since 2005, subscribers can purchase full versions of other articles of any issue only through the National Electronic Library. The editor of the Medical Radiology and Radiation Safety Journal in accordance with the National Electronic Library agreement has been providing the Library with all its production since 2005 until now.
The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.
Since 2017 the journal Medical Radiology and Radiation Safety has switched to digital identification of publications, assigning to each article the identifier of the digital object (DOI), which greatly accelerated the search for the location of the article on the Internet. In future it is planned to publish the English-language version of the journal Medical Radiology and Radiation Safety for its development. In order to obtain information about the publication activity of the journal in March 2015, a counter of readers' references to the materials posted on the site from 2005 to the present which is placed on the journal's website. During 2015 - 2016 years on average there were no more than 100-170 handlings per day. Publication of a number of articles, as well as electronic versions of profile monographs and collections in the public domain, dramatically increased the number of handlings to the journal's website to 500 - 800 per day, and the total number of visits to the site at the end of 2017 was more than 230.000.
The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Выпуски журналов
Medical Radiology and Radiation Safety. 2026. Vol. 71. № 1
DOI:10.33266/1024-6177-2026-71-1-113-120
N.K. Shandala, Yu.D. Udalov, Yu.E. Kvacheva, S.M. Kiselev,
V.A. Seregin, A.A. Filonova, S.M. Shinkarev, M.P. Semenova
20 Years of International Activity of the SRC-FMBC
A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: N.K. Shandala, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
The long-standing rich history of the SRC-FMBC includes a bright page of multilateral cooperation of the Center’s specialists with international research and medical organizations of the world. In the course of such cooperation, the experience accumulated in world practice is generalized; norms, rules, regulations, practical recommendations and guidelines for assuring the safety and protection of exposure to ionizing and non-ionizing radiation on personnel and the population are developed and improved. This article describes ways and perspectives of cooperation and collaboration with foreign partners.
Keywords: A.I. Burnazyan SRC-FMBC, international cooperation, regulation, radiation safety and protection, anniversary
For citation: Shandala NK, Udalov YuD, Kvacheva YuE, Kiselev SM, Seregin VA, Filonova AA, Shinkarev SM, Semenova MP. 20 Years of International Activity of the SRC-FMBC. Medical Radiology and Radiation Safety. 2026;71(1):113–120. (In Russian). DOI:10.33266/1024-6177-2026-71-1-113-120
References
1. FGBU GNTS FMBTS im. A.I.Burnazyana FMBA Rossii = FSBI State Scientific Center FMBC Named after A.I.Burnazyan FMBA of Russia. URL: https://fmbafmbc.ru/scientific-activities/SC-WOZ/ (Date of Access 25.06. 2025) (In Russ.).
2. FGBU GNTS FMBTS im. A.I.Burnazyana FMBA Rossii = FSBI State Scientific Center FMBC Named after A.I.Burnazyan FMBA of Russia. URL: https://fmbafmbc.ru/news/events_news/uchenye-tsentra-prinyali-uchastie-v-71-y-sessii-nkdar-oon (Date of Access 25.06.2025) (In Russ.).
3. Akleyev A.V., T.V. Azizova, Ivanov S.A., Kiselev S.M., Takhauov R.M., Fesenko S.V., Shinkarev S.M. Results of the 70th Session of the UN Scientific Committee on the Effects of Atomic Radiation (UNSCEAR) (Vienna, June 19–23, 2023). Meditsinskaya Radiologiya i Radiatsionnaya Bezopasnost’ = Medical Radiology and Radiation Safety. 2024;69;1:5-14 (In Russ.).
4. Sources, Effects and Risks of Ionizing Radiation United Nations Scientific Committee on the Effects of Atomic Radiation UNSCEAR 2020/2021. Report to the General Assembly with Scientific Annexes.
5. Publikatsiya 103 Mezhdunarodnoy Komissii po Radiatsionnoy Zashchite (MKRZ) = Publication 103 of the International Commission on Radiation Protection (ICRP). Ed. M.F.Kiselev, N.K.Shandala. Moscow, Alana Publ., 2009. 344 p. (In Russ.).
6. Lekomte Zh.-F., Solomon S., Takala Dzh., Yung T., Strand P., Myurit K., Kiselev S., Zhuo V., Shennon F., Yansens A. Radiologicheskaya Zashchita ot Oblucheniya Radonom = Radiological Protection Against Radon Exposure. Ed. M.V.Zhukovskiy, I.V.Yarmoshenko, S.M.Kiselev. Moscow, FMBTS im. A.I.Burnazyana FMBA Rossii Publ., 2015. 92 p. (In Russ.).
7. Tirmarsh M., Kharrison Dzh. D., Lur’ye D., Pak F., Blanshardon Ye., Marsh Dzh.V. Risk Vozniknoveniya Raka Legkogo pri Obluchenii Radonom i Produktami Yego Raspada. Zayavleniye po Radonu = Lung Cancer Risk from Radon and its Decay Products. Radon Statement. Ed. M.V.Zhukovskiy S.M.Kiselev, A.T.Gubin. Moscow, FMBTS im. A.I.Burnazyana FMBA Rossii Publ., 2013. 92 p. (In Russ.).
8. Kai M., Homma T., Lochard J., Schneider T., Lecomte J.F., Nisbet A., Shinkarev S., Averin V., Lazo T. ICRP. Radiological Protection of People and the Environment in the Event of a Large Nuclear Accident: Update of ICRP Publications 109 and 111. ICRP Publication 146. Ann. ICRP. 2020;49:4.
9. International Atomic Energy Agency, Criteria for Radionuclide Activity Concentrations for Food and Drinking Water, IAEA-TECDOC-1788. Vienna, IAEA, 2016.
10. International Atomic Energy Agency, Status of Radon Related Activities in Member States Participating in Technical Cooperation Projects in Europe, IAEA-TECDOC-1810. Vienna, IAEA, 2017.
11. Challenges in Nuclear and Radiological Legacy Site Management: Towards a Common Regulatory Framework, Radiological Protection: OECD Publishing. Paris, NEA, 2020. Doi: 10.1787/ccb40709-en.
12. Kotenko K.V., Kvacheva Yu.E., Granovskaya E.O., Koukhta B.A. Forensic Medical Aspects of Radiation Accidents Investigation. IAEA Int. Conference in Nuclear Forensics: Countering the Evolving Threat of Nuclear and Other Radioactive Material out of Regulatory Control. Vienna, 7-10 July 2014. IAEA-CN-218/85. P.102-103
13. Siegien K., Sneve M.K., Strand P., Shandala N.K., Romanov V., Semenova M. Regulatory Cooperation Program between the Norwegian Radiation and Nuclear Safety Authority and the Federal Medical Biological Agency of Russian Federation. Results and Review of Progress from 2015 to 2019. DSA Report, 2020.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was financed under the State Contract as part of the Federal Target Program «Nuclear and Radiation Safety for 2016‒2020 and for the period until 2030».
Contribution. Shandala N.K. – conception and design of the article, writing and editing the text; Udalov Yu.D. – conception and editing the text; Kvacheva Yu.E. – data collection and processing, writing the text; Kiselev S.M. – data collection and processing, writing the text; Seregin V.A. – data collection and processing, writing the text; Filonova A.A. – data collection and processing, writing the text; Semenova M.P. – translation into English, editing the text.
Article received: 20.11.2025. Accepted for publication: 25.12.2025.
Medical Radiology and Radiation Safety. 2026. Vol. 71. № 1
DOI:10.33266/1024-6177-2026-71-1-14-20
S.N. Lukyanova, N.I. Karpikova, I.A. Veselovsky, T.V. Fomina
Study of the State of the Nervous System
of Workers in the Near Zone of Microwave Em Non-Thermal Intensity
A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: S.N. Lukyanova, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To assess the neurological status of personnel who have worked for a long time in the near zone of low-intensity packet-pulse microwave electromagnetic fields.
Material and methods: The subjects were researchers from the All-Russian Institute of Experimental Physics (VNIIF), who worked with devices generating microwave electromagnetic fields (53 individuals and a corresponding control group of 30). These were men aged 24 to 60 years, with work experience ranging from 1 to 35 years. The nature of exposure was a pulse-packet mode of microwave electromagnetic fields in the near zone, with oscillations of the electric field at the workplace ranging from 0.34 µW/cm² to 314 µW/cm². Both groups underwent examination, including a general clinical examination, registration, and assessment of rheoencephalography (REG), electroencephalography (EEG), and rheography (RVG).
Results: Analysis of clinical symptoms shows a statistically significant prevalence of complaints of headache, weakness, fatigue, and irritability in the main group compared to the control group. In 86.8 % of those working under microwave electromagnetic fields, a decrease in tendon and periosteal reflexes was observed. Compared to the control group, there were statistically significant prevalences of: increased blood pressure and its asymmetry, reduced blood filling of all vessels, their dystonia according to REG data, and in EEG – increased desynchronization and disruption of alpha activity rhythm. The changes in the analyzed indicators are shown depending on the length of service for the periods: 1–5 years, 6–15 years, 16–25 years, 26–35 years.
Conclusion: As a result of the examination, a conclusion was made about the possibility of the development of neurological disorders in workers under the specified conditions of microwave electromagnetic fields, manifested as syndromes: vegetative-vascular dystonia, vestibulopathy, polyneuropathy, and asthenia, which increased with the length of service. The obtained results are important for accumulating data on the bioeffects of weak electromagnetic fields, with the aim of understanding the mechanisms and phenomenology of possible deviations in the state of the body, which is necessary for developing methodologies for the prevention and treatment of this personnel.
Кеywords: personel, microwave electromagnetic fields, near zone, non-thermal intensity, medical examination, nervous system, state of the body, dependence on work experience
For citation: Lukyanova SN, Кarpikova NI, Veselovsky IA, Fomina TV. Study of the State of the Nervous System of Workers in the Near Zone of Microwave Em Non-Thermal Intensity. Medical Radiology and Radiation Safety. 2026;71(1):14–20. (In Russian). DOI:10.33266/1024-6177-2026-71-1-14-20
References
1. Gordon Z.V. Voprosy Gigiyeny Truda i Biologicheskogo Deystviya Elektromagnitnykh Poley Sverkhvysokikh Chastot = Issues of Occupational Hygiene and Biological Effects of Ultra-High Frequency Electromagnetic Fields. Leningrad, Meditsina Publ., 1966. 163 p. (In Russ.).
2. Sadchikova M.N., Glotova N.V., Snegova G.V., Korenevskaya S.P. Clinic and Treatment of Radio Wave Disease. Gigiyena Truda i Biologicheskoye Deystviye Elektromagnitnykh Poley Radiochastot = Occupational Hygiene and Biological Action of Electromagnetic Fields of Radio Frequencies. Moscow Publ., 1972. P. 81-93 (In Russ.).
3. Nikonova K.V., Vermel’ A.Ye. Clinical and Hygienic Problems of the Influence of Electromagnetic Fields of the Radio Frequency Range. Fiziologiya Cheloveka i Zhivotnykh = Human and Animal Physiology. 1978;22:112–139 (In Russ.).
4. Shandala M.G. Physiological Effects of Exposure to Low-Intensity Microwave Fields on the Body. Gigiyena Naselonnykh Mest = Hygiene of Populated Areas. 1979;18:53-58 (In Russ.).
5. Makri D.I. Research Conducted in the USSR and Eastern European Countries in the Field of Biological Effects of Microwave Radiation. TIIER. 1980;68;1:96-104 (In Russ.).
6. Kholodov Yu.A. Reaktsiya Nervnoy Sistemy na EMP = Reaction of the Nervous System to EMF. Moscow, Nauka Publ., 1975. 284 p. (In Russ.).
7. Vorontsov I.V., Zhilyayev Ye.G., Karpov V.N., Ushakov I.B. Malyye Radiatsionnyye Vozdeystviya i Zdorov’ye Cheloveka (Ocherki Sistemnogo Analiza) = Minor Radiation Effects and Human Health (Essays on Systems Analysis). Moscow, Voronezh, Voronezhskiy Gosudarstvennyy Universitet Publ., 2002. 256 p. (In Russ.).
8. Luk’yanova S.N., Makarov V.P., Rynskov V.V. Dependence of Changes in the total Bioelectrical Activity of the Brain on Low-Intensity MW Irradiation on the Energy Flux Density. Radiatsionnaya Biologiya. Radioekologiya = Radiation Biology. Radioecology. 1996;36;5:706-710 (In Russ.).
9. Kurenkova G.V. Neioniziruyushchiye Elektromagnitnyye Izlucheniya kak Neblagopriyatnyy Faktor Proizvodstvennoy Sredy = Non-Ionizing Electromagnetic Radiation as an Unfavorable Factor in the Industrial Environment. Irkutsk, Irkutskiy Gosudarstvennyy Meditsinskiy Universitet Publ., 2013. 98 p. (In Russ.).
10. Titova L.K. Elektromagnitnoye Izlucheniye - Vred ili Pol’za = Electromagnetic Radiation - Harm or Benefit. Ekaterinburg, Ural’skiy Gosudarstvennyy Lesotekhnicheskiy Universitet Publ., 2014. 33 p. (In Russ.).
11. Pryakhin Ye.A. Adaptatsionnyye Reaktsii na Subkletochnom, Kletochnom, Sistemnom i Organizmennom Urovnyakh pri Vozdeystvii Elektromagnitnykh Poley = Adaptation Reactions at the Subcellular, Cellular, Systemic and Organismal Levels when Exposed to Electromagnetic Fields. Extended Abstract of Doctor’s Thesis (Med.). Chelyabinsk Publ., 2007. 75 p. (In Russ.).
12. Zhavoronkov L.P., Dubovik B.V., Pavlova L.N., Kolganova O.I., Posadskaya V.M. Effect of Low-Intensity Broadband Pulse-Modulated EMF on General CNS Excitability. Radiatsiya i Risk = Radiation and Risk. 2011;20;2:64-74 (In Russ.).
13. Pavlova L.N., Zhavoronkov L.P., Dubovik B.V. The Influence of Low-Intensity, Broadband Pulse-Modulated EMF on the Cognitive Functions of the Rat Brain. Radiatsiya i Risk = Radiation and Risk. 2013;22;2:91–100 (In Russ.).
14. Luk’yanova S.N. Fundamental’naya Kharakteristika Neyroeffektov Slabykh Elektromagnitnykh Vozdeystviy (ot Neyrona k Otdelu Mozga, TSNS, Organizmu) = Fundamental charact``eristics of the Neuronal Effects of Weak Electromagnetic Influences (from Neuron to Brain Region, Central Nervous System, Organism). Moscow, FMBTS im. A.I.Burnazyana FMBA of Russia Publ., 2023. 140 p. (In Russ.). ISBN 978-5-93064-225-4.
15. Karpikova N.I. Kliniko-Neyrofiziologicheskoye Issledovaniye Sostoyaniya Nervnoy Sistemy Rabotayushchikh v Blizhney Zone Pachechno-Impul’snogo SVCH-Oblucheniya Nizkoy Intensivnosti = Clinical and Neurophysiological Study of the State of the Nervous System of Workers in the Near Zone of Low-Intensity Burst-Pulse Microwave Irradiation. Doctor’s Thesis (Med.). Moscow, Institut Biofiziki Publ., 1995 (In Russ.).
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Research concept and design, data acquisition and processing – S.N. Lukyanova, N.I. Karpikova. Analysis and interpretation of the results – S.N. Lukyanova, T.V. Fomina, I.A. Veselovsky. Approval of the manuscript for publication – S.N. Lukyanova.
Article received: 20.11.2025. Accepted for publication: 25.12.2025.
Medical Radiology and Radiation Safety. 2025. Vol. 70. № 6
DOI:10.33266/1024-6177-2025-70-6-5-11
A.V. Akleyev1, 2,T.V. Azizova1, S.A. Ivanov3, S.M. Kiselev4, E.M. Melikhova5,
S.V. Fesenko6, S.M. Shinkarev4
Results of the 72Nd Session of the United Nations Scientific Committee on the Effects of the Atomic Radiation (Unscear) (Vienna, 16‒20 June, 2025)
1 Southern Urals Federal Research and Clinical Center for Medical Biophysics, Chelyabinsk, Russia
2 Chelyabinsk State University, Chelyabinsk, Russia
3 А. Tsyb Medical Radiological Research Centre, Obninsk, Russia
4 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
5 Nuclear Safety Institute, Moscow, Russia
6 Russian Institute of Radiology and Agroecology of National Research Centre «Kurchatov Institute», Obninsk, Russia
Contact person: A.V. Akleyev, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
The paper gives a brief overview of the 72st Session of the United Nations Scientific Committee on the Effects of Atomic Radiation
(UNSCEAR) which took place during 16-20 June 2025. National delegations from 29 UNSCEAR Member-states as well as the representatives of nine international organizations participated in the work of the Session. Within the framework of the meetings of the working group and subgroups the following documents have been discussed: R.767 “Epidemiological studies of ionizing radiation and cancer” and R.768 “Effects of ionizing radiation on the circulatory system”. The Committee has also considered the progress made on the document R.769 “Effects of ionizing radiation on the nervous system”. The following Reports of the Secretariat have also been presented during the work of the Session: UNSCEAR/72/7 “Implementation of public information and outreach strategy for 2025–2029”. UNSCEAR/72/8 “Implementation of the Committee’s Future Programme of Work (2025–2029), including consideration of the Committee’s ad hoc working group on effects and mechanisms”, UNSCEAR/72/9 “Implementation of the Strategy to improve collection, analysis and dissemination of data on radiation exposure, including consideration of the Committee’s ad hoc working group on sources and exposure”. Report to the UN General Assembly has been prepared based on the results of the Session.
Keywords: UNSCEAR, 72nd Session, dose, cancer, diseases of the circulatory system, nervous system, radiation risk
For citation: Akleyev AV, Azizova TV, Ivanov SA, Kiselev SM, Melikhova EM, Fesenko SV, Shinkarev SM. Results of the 72Nd Session of the United Nations Scientific Committee on the Effects of the Atomic Radiation (Unscear) (Vienna, 16‒20 June, 2025). Medical Radiology and Radiation Safety. 2025;70(6):5–11. (In Russian). DOI:10.33266/1024-6177-2025-70-6-5-11
References
1. Akleyev AV, Azizova TV, Ivanov SA, Kiselev SM, Takhauov RM, Fesenko SV, Shinkarev SM. Results of the 70-th Session of the United Nations Scientific Committee on the Effects of the Atomic Radiation (UNSCEAR) (Vienna, 19–23 June, 2023). Medical Radiology and Radiation Safety. 2024;69(1):5–14 (In Russ) http://doi.org/10.33266/1024-6177-2024-69-1-5-14.
2. Akleyev AV, Azizova TV, Karpikova LA, Kiselev SM, Kononenko DV, Melikhova EM, Romanov VV, Romanov SA, Takhauov RM, Usoltsev VYu, Shinkarev SM. Results of the 69th Session of the United Nations Scientific Committee on the Effects of the Atomic Radiation (UNSCEAR) (Vienna, 9‒13 May, 2022). Medical Radiology and Radiation Safety. 2022;67(5):24–32 (In Russ) http://doi.org/10.33266/1024-6177-2022-67-5-24-32.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.07.2025. Accepted for publication: 25.08.2025.
CONTENTS № 1 - 2026
View or download the full issue in PDF (Russian)
| RADIATION BIOLOGY |
5 |
Kiseleva D.A., An’kov S.V., Zhukova N.A., Borisova M.S., Bogatisheva K.S., Tolstikova T.G., Melchenco M.A., Yarovaya O.I. |
|||
| RADIATION MEDICINE |
14 |
Lukyanova S.N., Кarpikova N.I., Veselovsky I.A., Fomina T.V. |
|||
|
21 |
Abdullaev S.A., Raeva N.F., Fomina D.V., Kalinin T.P., Maksimova T.N., Zasukhina G.D. |
||||
|
26 |
Kretov A.S., Yarotskaya A.A., Torubarov F.S., Parinov O.V., Metlyaeva N.A., Kasymova O.A., Vlasova I.V., Podgornova O.V., Vodolazskaya E.V., |
||||
| NUCLEAR
MEDICINE |
33 |
Bragina O.D., Ivanova A.G., Larkina M.S., Varvashenya R.N.,Vostrikova M.A., Popova N.O., Goldberg V.E., Chernov V.I., Patalyak S.V. |
|||
|
39 |
Kaidash Yu.A., Shaidiyarova E.L., Baryshnikov I.A., Sychev P.V., |
||||
|
42 |
Labushkina A.A., Astrelina T.A., Udalov Yu.D., Kodina G.E. |
||||
|
49 |
Opportunities of Radionuclide Diagnostics with 99mТc-MIBI in Children with Tuberculosis Mikhaylov S.G., Perfiliev A.V., Yukhimenko N.V. |
||||
|
57 |
Ussov W.Yu., Minin S.M., Anashbaev Zh.Zh., Samoilova E.A., Sazonova S.I., Grishkov A.V., Sorokina K.N., Borisenko A.P., Lishmanov Yu.B., |
||||
|
67 |
Tishchenko V.K., Fedorova A.V., Chibisova O.F., Morozova N.B., |
||||
|
RADIATION DIAGNOSTICS |
73 |
Anatomical Configuration of Mandibular Incisors: a Cone Beam Computed Tomography Study Aqeel Al-Saedi, Bahaa Al-Bakhakh, Riad Al-Taee, Ali Ayad Swadi |
|||
|
82 |
Adnexal Masses Insight: The Role of the Diffusion-Weighted MRI Najah Raham Rashi, Yusur Muhammad Said Thabit Al-Rawi, Khaleel Akeash Hadi |
||||
|
90 |
Afukova O.A., Fedyanin M.Yu., Yudin A.L., Vinokurov A.S., Kovaleva A.S., Kotova E.V., Donchenko N.S. |
||||
|
96 |
Matkevich E.I. |
||||
| RADIATION
THERAPY |
106 |
Mushkarina T.Yu., Kuzmina E.G., Grivtsova L.Yu., Afonin G.V., Biryukov V.A., Ivanov S.A., Kaprin A.D. |
|||
| ANNIVERSARY |
113 |
20 Years of International Activity of the SRC-FMBC Shandala N.K., Udalov Yu.D., Kvacheva Yu.E., Kiselev S.M., Seregin V.A., Filonova A.A., Shinkarev S.M., Semenova M.P. |
|||
Medical Radiology and Radiation Safety. 2025. Vol. 70. № 6
DOI:10.33266/1024-6177-2025-70-6-12-19
A.A. Melnikova1, 2, A.A. Afonin1, L.N. Komarova1
Integrative Analysis of Pmaip1 and Birc5 Gene Expression, as Well as Protein-Protein Interactions in Sk-N-Be(2) Neuroblastoma Cells in Combination Therapy
1 National Research Nuclear University MEPhI, Obninsk, Russia
2 A.F. Tsyb Medical Radiological Research Center, Obninsk, Russia
Contact person: A.A. Melnikova, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: Evaluation of the effect of ionizing radiation with various linear energy transfer on the expression of the PMAIP1 (Noxa) and BIRC5 (Survivin) genes to elucidate the molecular mechanisms determining the radiosensitivity of tumor cells.
Material and methods: The object of the study was SK-N-BE(2) cells. Four study groups were formed: a group exposed to ionizing radiation; a group treated with doxorubicin; a group of combined exposure to ionizing radiation and doxorubicin; and an untreated control group.
The cells were irradiated with 12C ions at the U-70 accelerator of the Institute for High Energy Physics (IHEP) of the National Research Centre «Kurchatov Institute» (Protvino, Moscow Region).
The irradiation was carried out at a dose of 4 Gy in an aqueous phantom with an average energy of 455 MeV/nucleon. The average LET of radiation in the initial phase was 11 keV/µm, with a peak of 120‒140 keV/µm. The cells were also irradiated with gamma radiation on the basis of the Russian Institute of Radiology and Agroecology at the unique scientific facility «Gamma radiation irradiation unit GUR-120» (radiation source 60Co, 1.25 MeV) at a dose of 4 Gy. The dose rate was 0.9 Gy /min. The cells were treated with the chemotherapy drug doxorubicin at a concentration of 0.004 mg/ml 24 hours before irradiation. Total RNA was isolated using an RNA Solo kit and quantified spectrophotometrically (NanoDrop ND-1000). Reverse transcription and amplification were performed simultaneously in real time using the OneTube RT-PCR kit with SYBR Green I as a fluorescent indicator. Key regulatory targets were identified from a set of common targets using STRING (protein-protein interaction network analysis, Homo sapiens), visualized and analyzed in Cytoscape v3.10.3 (CytoHubba plugin), and functionally annotated using Metascape (GO and KEGG pathway analysis, p<0,01).
Results: In SK-N-BE(2) cells, doxorubicin demonstrated a significant decrease in the expression of the BIRC5 gene, which is consistent with its known proapoptotic activity. The combined effect of gamma radiation (4 Gy) and doxorubicin demonstrated an additive effect (0,04). Irradiation with 12C ions demonstrated a significant decrease in BIRC5 expression with monotherapy (0,02), but a less pronounced decrease in the case of combined action with doxorubicin (0,10). Doxorubicin induced the expression of the PMAIP1 gene (0.16), this effect was synergistically enhanced when combined with gamma radiation (0.25), but was suppressed when using 12C ions (0.05), which is probably due to the activation of antiapoptotic mechanisms.
Conclusion: The results of the analysis of Gene Ontology and gene expression in SK-N-BE(2) cells confirmed the functional specialization of PMAIP1 (mitochondrial apoptosis) and BIRC5 (cell cycle regulation). Doxorubicin and gamma radiation enhanced apoptosis by affecting these genes, while 12C ions activated DNA repair, suppressing PMAIP1 and weakening the effect on BIRC5.
Keywords: 12C ion radiation therapy, gamma therapy, Bcl-2 family proteins, SK-N-BE (2), doxorubicin, BIRC5, PMAIP1, gene ontology
For citation: Melnikova AA, Afonin AA, Komarova LN. Integrative Analysis of Pmaip1 and Birc5 Gene Expression, as Well as Protein-Protein Interactions in Sk-N-Be(2) Neuroblastoma Cells in Combination Therapy. Medical Radiology and Radiation Safety. 2025;70(6):12–19. (In Russian). DOI:10.33266/1024-6177-2025-70-6-12-19
References
1. Roufayel R., Younes K., Al-Sabi A., Murshid N. BH3-Only Proteins Noxa and Puma Are Key Regulators of Induced Apoptosis. Life (Basel). 2022;12;2:256. doi:10.3390/life12020256.
2. Greaves G., Milani M., Butterworth M., Carter R.J., Byrne D.P., Eyers P.A., Luo X., Cohen G.M., Varadarajan S. BH3-Only Proteins Are Dispensable for Apoptosis Induced by Pharmacological Inhibition of Both MCL-1 and BCL-X L. Cell Death Differ. 2019;26:1037-1047. doi: 10.1038/s41418-018-0183-7.
3. Huang K., O’Neill K.L., Li J., Zhou W., Han N., Pang X., Wu W., Struble L., Borgstahl G., Liu Z. BH3-Only Proteins Target BCL-XL/MCL-1, Not BAX/BAK, to Initiate Apoptosis. Cell Res. 2019;29:942-952. doi: 10.1038/s41422-019-0231-y.
4. Rafatmanesh A., Behjati M., Mobasseri N., Sarvizadeh M., Mazoochi T., Karimian M. The Survivin Molecule as a Double-Edged Sword in Cellular Physiologic and Pathologic Conditions and its Role as a Potential Biomarker and Therapeutic Target in Cancer. J Cell Physiol. 2020;235;2:725-744. doi:10.1002/jcp.29027.
5. George R., Hehlgans S., Fleischmann M., Rödel C., Fokas E., Rödel F. Advances in Nanotechnology-Based Platforms for Survivin-Targeted drug Discovery. Expert Opin Drug Discov. 2022;17;7:733-754. doi:10.1080/17460441.2022.2077329.
6. Wijaya W., Phyu S.M., Jiang S. Extracellular Vesicle (EV) Survivin for Cancer Diagnostics and Therapeutics: A Review. Front Biosci (Landmark Ed). 2024;29;8:302. doi:10.31083/j.fbl2908302.
7. Liao J., Qing X., Li X., et al. TRAF4 Regulates Ubiquitination-Modulated Survivin Turnover and Confers Radioresistance. Int J Biol Sci. 2024;20;1:182-199. doi:10.7150/ijbs.87180.
8. Fang X.L., Cao X.P., Xiao J., et al. Overview of Role of Survivin in Cancer: Expression, Regulation, Functions, and its Potential as a Therapeutic Target. J Drug Target. 2024;32;3:223-240. doi:10.1080/1061186X.2024.2309563.
9. Martínez-Sifuentes M.A., Bassol-Mayagoitia S., Nava-Hernández M.P., et al. Survivin in Breast Cancer: A Review. Genet Test Mol Biomarkers. 2022;26;9:411-421. doi:10.1089/gtmb.2021.0286.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. A.A. Melnikova – conducting experiments, research development; A.A. Afonin – conducting experiments; L.N. Komarova – development of the research concept, scientific supervision.
Article received: 20.07.2025. Accepted for publication: 25.08.2025.