Medical Radiology and Radiation Safety. 2021. Vol. 66. № 6. P. 26–33

Analysis of the Appearance of Micronuclei in the Erythrocytes and Activity of Bone Marrow Cells Proliferation after the Prolonged Low Dose Fast Neutrons Irradiation of Mice

E.Yu. Moskaleva, A.N. Romantsova, Yu.P. Semochkina, A.V. Rodina, I.V. Cheshigin, A.S. Degtyarev, A.S. Zhirnik

National Research Center «Kurchatov Institute», Moscow, Russia

Contact person: Elizaveta Yurievna Moskaleva: This email address is being protected from spambots. You need JavaScript enabled to view it. ; This email address is being protected from spambots. You need JavaScript enabled to view it.  

ABSTRACT

Purpose: To analyze the level of cytogenetic damage and the activity of bone marrow cells proliferation in C57BL/6 mice after prolonged fast neutrons low dose irradiation at 10–500 mGy. 

Material and methods: Male C57BL/6 mice at the age of 7–8 and 16 weeks were used in the experiments. Irradiation was carried out on an OR-M installation in the field of fast neutrons and gamma quanta using five Pu(α,n)Be radionuclide sources with a high fast neutron yield at a dose rate of 2.13 mGy/h. The frequency of polychromatophilic (PCE) and normochromic (NCE) erythrocytes with micronuclei (MN) and the ratio of PCE and NCE were analyzed using light microscopy after cytochemical staining of the bone marrow cells of control and irradiated mice. The proliferation activity of bone marrow cells was determined by the number of Ki-67+-cells. The parameters of the cell cycle and the level of apoptosis were studied after DNA staining with DAPI using flow cytometry. Statistical processing of the results was carried out according to the Student’s method using the computer program Origin.

Results: It was found that prolonged irradiation of mice with fast neutrons at a low dose rate (2.13 mGy/h) at doses from 10 to 500 mGy after 24 h led to statistically significant increase in the frequency of PCE with MN at all studied doses. No dose dependence of this parameter was observed in the studied range. The increase in the frequency of PCE with MN at a dose of 500 mGy was prolonged and persisted for at least 72 h. A significant increase in the frequency of NCE with MN 24 h after irradiation was found only at a dose of 500 mGy, which persisted up to 48 h. At this dose, there was also a decrease in the number of nucleated cells in the bone marrow 24 – 72 h after exposure, a decrease in the number of Ki-67+-cells 24 h after irradiation of mice, a block of the cell cycle in the G2/M phase, and a decrease of cells in the G0/G1 phase, but after 48 h, there were no disturbances in the cell cycle. 

Conclusion: It has been shown that after a single total prolonged irradiation of mice at low doses (10–500 mGy), when analyzing the frequency of PCE with MN, cytogenetic damage is recorded in the bone marrow, which indicates the genetic danger of exposure to even such low levels of fast neutron irradiation. A decrease in Ki67+ cells and cell cycle arrest at the G2/M phase were found only after irradiation of mice at a dose of 500 mGy and only 24 h after exposure, while the number of nucleated cells in the bone marrow at this dose was reduced, at least to 72 h.

Key words: micronuclei, bone marrow, cell cycle, cell proliferation, Ki-67, fast neutrons, prolonged irradiation, low dose, mice

For citation: Moskaleva EYu, Romantsova AN, Semochkina YuP, Rodina AV, Cheshigin IV, Degtyarev AS, Zhirnik AS. Analysis of the Appearance of Micronuclei in the Erythrocytes and Activity of Bone Marrow Cells Proliferation after the Prolonged Low Dose Fast Neutrons Irradiation of Mice. Medical Radiology and Radiation Safety. 2021;66(6):26–33.

DOI: 10.12737/1024-6177-2021-66-6-26-33

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 PDF (RUS) Full-text article (in Russian)

Conflict of interest. The authors declare no conflict of interest.

Financing. The study had no sponsorship.

Contribution. Article was prepared with equal participation of the authors.

Article received: 10.08.2021 

Accepted for publication: 21.09.2021.

Medical Radiology and Radiation Safety. 2021. Vol. 66. № 6. P. 39–44

Comparative Analysis of Paired Correlations Structure Between Psychological Scales
in Relation to Groups of Different Sizes

A.A. Kosenkov 

A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia

Contact person: Alexander Kosenkov: This email address is being protected from spambots. You need JavaScript enabled to view it.  

ABSTRACT

Purpose: To propose a procedure for comparative analysis of correlation relationships structure between psychological scales in samples of different sizes.

Material and methods: A procedure of comparative analysis of correlation relationships structure between psychological indicators in groups of different sizes based on the «zet» method of R. Fisher is proposed. To illustrate the method, data from psychodiagnostic surveys of the nuclear power plant (NPP) personnel who worked in normal conditions and the personnel of the Chernobyl NPP at four different stages of aftermath activities in 1986–1987 were used. All the subjects performed the Minnesota Multiphasic Personality Inventory (MMPI); the results of each of the groups were subjected to a correlation analysis using the Pearson method. The analysis took into account average (0.5 ≤ r < 0.7) and strong (r ≥ 0.7) correlations.

Results: Using the example of a number of psychodiagnostic examinations of the Chernobyl NPP personnel at various stages of aftermath activities, it is shown that the structure of correlation relationships between the MMPI scales can reflect the peculiarities of mental adaptation of professional teams working in regular and extreme conditions.

It was shown that in the aftermath process by March–April 1987, the number of medium and strong pairwise Pearson correlations between the clinical MMPI scales increased. This fact apparently indicates that during this period, the NPP personnel were characterized by the simultaneous activation of various mechanisms of intrapsychic adaptation. The core of the correlation pleiad consisted of scales 1, 2, 7, and 8. Such a multidirectional reaction to the aftermath stressors was accompanied by a statistically significant increase in almost all clinical scales (except scale 6) of the average MMPI profile in comparison with the control group. The absence of stable correlations of clinical scales with the F scale indicates that the marked increase in the MMPI profile was not associated with a tendency to aggravation.

By the period of November–December 1987, the hypochondria scale occupied a leading place in the correlation pleiad of the MMPI indicators of the Chernobyl NPP personnel, the number of its significant connections with other clinical scales (2, 3, 7 and 8) reached four. Apparently, the mechanism of anxiety somatization at that time could be considered as a syndrome-forming factor and taken into account when planning rehabilitation and health measures.

Conclusion: The use of the proposed method of the strength standardization of pairwise correlation relationships between the MMPI scales allowed us to legitimately compare these indicators in groups that differ significantly in number. The chosen representation form of correlations facilitates the analysis of their structure.

Key words: Pearson’s correlation coefficient, correlation pleiad, psychic adaptation, MMPI, Chernobyl NPP

For citation: Kosenkov A.A. Comparative Analysis of Paired Correlations Structure Between Psychological Scales in Relation to Groups of Different Sizes. Medical Radiology and Radiation Safety. 2021;66(6): 39–44.

DOI: 10.12737/1024-6177-2021-66-4-39-44

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Conflict of interest. The author declare no conflict of interest.

Financing. The study had no sponsorship.

Contribution. The article was prepared by one author.

Article received: 18.09.2021

Accepted for publication: 22.10.2021

Medical Radiology and Radiation Safety. 2021. Vol. 66. № 6. P. 34–38

Influence of Drinking Water Quality on the Current of Acute Radiation Disease in Mice

Bychkova T.M.^1,2, Andrianova I.E.¹, Nikitenko O.V.^1,2, Stavrakova N.M.¹, Parfenova I.M.¹, Karaulova T.A.¹, Gordeev A.V.¹, Ivanov A.A.^1,2,3

¹A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia

²RFSSC Institute of Biomedical Problems, Moscow, Russia

³Joint Institute for Nuclear Research, Dubna, Russia

Contact person: Taisia Mikhailovna Bychkova: This email address is being protected from spambots. You need JavaScript enabled to view it.  

ABSTRACT

Purpose: Assessing the role of various factors in the formation of radioresistance is an important branch of radiobiology. The quality of drinking water, as it turned out, can significantly affect radioresistance. Against the background of studying the antiradiation properties of various types of water, differing in mineral and isotopic composition, the problem of the influence of tap water on the course of radiation injury remained underestimated. This circumstance determined the purpose of the work: to evaluate the modifying effect of tap water on the course of acute radiation sickness after X-ray irradiation of mice at an average lethal dose. 

Material and methods: Female ICR (CD-1) mice were irradiated with an average lethal dose once – 6.5 Gy of X-ray irradiation. After irradiation, half of the mice received tap water as drinking water, and the other half received artificially mineralized drinking water.

Results: Keeping animals on tap water significantly reduced the survival rate of mice both with a single dose (log-rank test p=0.02, χ2=5.38) compared with animals receiving artificially mineralized distilled water. In addition, in the group of mice that received tap water, an increase in the rate of death of mice and a lower preservation of the group mass of animals during the development of acute radiation injury was noted. 

Conclusion: Tap water, used as drinking water, increases the damaging effect of radiation when X-rays are irradiated in mice.

Key words: tap water, artificially mineralized distilled water, X-ray irradiation, survival rate, mortality rate, mice, model

For citation: Bychkova TM, Andrianova IE, Nikitenko OV, Stavrakova NM, Parfenova IM, Karaulova TA, Gordeev AV, Ivanov AA. Influence of Drinking Water Quality on the Current of Acute Radiation Disease in Mice. Medical Radiology and Radiation Safety. 2021;66(6):34–38.

DOI: 10.12737/1024-6177-2021-66-6-34-38

References

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 PDF (RUS) Full-text article (in Russian)

Conflict of interest. The authors declare no conflict of interest.

Financing. The study had no sponsorship.

Contribution. Article was prepared with equal participation of the authors.

Article received: 10.08.2021

Accepted for publication: 21.09.2021

Medical Radiology and Radiation Safety. 2021. Vol. 66. № 6. P. 45–49

Psychophysiological Adaptation of a Patient with Acute Radiated Sickness
of Average Degree and Acute Leukemia

N.A. Metlyaeva, A.Yu. Bushmanov, I.А. Galstyan, A.A. Davtyan, M.Yu. Sukhova, E.S. Skorobogatykh, O.V. Shcherbatykh

A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia

Contact person: Nelya Andreevna Metlyaeva: This email address is being protected from spambots. You need JavaScript enabled to view it.  

ABSTRACT

Purpose: Clinical and psychophysiological assessment of the adaptation of a patient who suffered from acute radiation sickness of moderate severity after the Chernobyl accident and acute leukemia, which began 30 years after the transferred ARS.

Material and methods: A clinical and psychophysiological examination of a patient who suffered from acute radiation sickness of moderate severity after the Chernobyl accident and acute leukemia that occurred 30 years after the Chernobyl accident was carried out. The object of the study was the patient D.R.I., born in 1950, deputy. Head of the Chernobyl NPP workshop, participant in the liquidation of the consequences of the 1986 Chernobyl accident. On April 26, 1986, during an emergency, he underwent relatively uniform beta-gamma radiation with the development of ARS II (moderate) severity. Within 3.5 hours after the accident was in the premises of the Chernobyl nuclear power plant. In the structure of ARS, bone marrow syndrome of the second degree and oropharyngeal syndrome of the first degree of severity were observed. The radiation dose, according to a cytogenetic study, was 3.4 Gy. A psychophysiological study was carried out using the MMPI methodology, the Cattell’s 16 Personality Factors Test, the Expert automated software and methodological complex, designed to study the personality characteristics of a person, the cognitive and intellectual characteristics of a person, the Raven’s Progressive Matrices, simple and complex sensorimotor reactions and reactions to a moving object, 15 (2001) and 30 years (2016) after the Chernobyl radiation accident.

 Results: The clinical and psychophysiological assessment of the personality and the actual mental state made it possible to determine the demonstrative-hypochondriacal type of disturbance in the psychophysiological adaptation with the tendency for the anxiety-depressive behavior of the patient to progress to a state of increasing depression, more pronounced in dynamics.

 Conclusion: The psychophysiological assessment of the personality and current mental state of the patient who had ARS, moderate and, 30 years after the Chernobyl accident, acute leukemia showed a demonstrative-hypochondria type of adaptation disorder, as a variant of a disharmonious combination of hypochondria, anxiety-depressive and demonstrative tendencies with a predominance of demonstrativeness (the first, second and third scale of the MMPI methodology) with a significant decrease on the ninth scale in the form of an increase in depression in dynamics. The prevalence of demonstrativeness over growing depression in a person with high intellect, good figurative and logical thinking, and a lack of sensorimotor inhibition is a manifestation of optimism and resistance to a serious illness.

Key words: acute radiation sickness, ionizing radiation, asthenia, depression, adaptation 

For citation: Metlyaeva NA, Bushmanov AYu, Galstyan IА, Davtyan AA, Sukhova MYu, Skorobogatykh ES, Shcherbatykh OV. 

sychophysiological Adaptation of a Patient with Acute Radiated Sickness of Average Degree and Acute Leukemia. Medical Radiology and Radiation Safety. 2021;66(6): 45–49.

DOI: 10.12737/1024-6177-2021-66-6-45-49

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Conflict of interest. The authors declare no conflict of interest.

Financing: research topic "Features of the subacute course of radiation sickness", code: "Subacute radiation sickness".

Contribution: development of the research concept, data analysis and interpretation of the results, writing the text of the article - N.A. Metlyaeva; development of the theoretical basis of the research - Bushmanov A.Yu., Galstyan I.A .; medical examination of the patient - Davtyan A.A., Sukhova M.Yu., Skorobogatykh E.S .; collection and analysis of literary material, text editing - Shcherbatykh O.V.

Article received: 16.04.2021. 

Accepted for publication: 21.08.2021.