JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.
Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.
The journal is included in the List of Russian Reviewed Scientific Journals of the Higher Attestation Commission. Since 2008 the journal has been available on the Internet and indexed in the RISC database which is placed on Web of Science. Since February 2nd, 2018, the journal "Medical Radiology and Radiation Safety" has been indexed in the SCOPUS abstract and citation database.
Brief electronic versions of the Journal have been publicly available since 2005 on the website of the Medical Radiology and Radiation Safety Journal: http://www.medradiol.ru. Since 2011, all issues of the journal as a whole are publicly available, and since 2016 - full-text versions of scientific articles. Since 2005, subscribers can purchase full versions of other articles of any issue only through the National Electronic Library. The editor of the Medical Radiology and Radiation Safety Journal in accordance with the National Electronic Library agreement has been providing the Library with all its production since 2005 until now.
The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.
Since 2017 the journal Medical Radiology and Radiation Safety has switched to digital identification of publications, assigning to each article the identifier of the digital object (DOI), which greatly accelerated the search for the location of the article on the Internet. In future it is planned to publish the English-language version of the journal Medical Radiology and Radiation Safety for its development. In order to obtain information about the publication activity of the journal in March 2015, a counter of readers' references to the materials posted on the site from 2005 to the present which is placed on the journal's website. During 2015 - 2016 years on average there were no more than 100-170 handlings per day. Publication of a number of articles, as well as electronic versions of profile monographs and collections in the public domain, dramatically increased the number of handlings to the journal's website to 500 - 800 per day, and the total number of visits to the site at the end of 2017 was more than 230.000.
The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Issues journals
Medical Radiology and Radiation Safety. 2024. Vol. 69. № 5
DOI:10.33266/1024-6177-2024-69-5-15-20
S.M. Rodneva1, L.P. Sycheva1, A.A. Maksimov1, E.S. Zhorova1, A.A. Tsishnatti1,
G.S. Tishchenko1, Yu.A. Fedotov1, 2, T.M. Trubchenkova1, E.I. Yashkina1,
D.V. Guryev1, V.G. Barchukov1
Genotoxic Effects in Spleen and Bone Marrow of Wistar Rats Chronically Exposed to Tritium Oxide and 3H-Thymidine with Drinking Water
1 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2 N.N. Semenov Federal Research Center for Chemical Physics, Moscow, Russia
Contact person: Sofya Mikhailovna Rodneva, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: The operation of nuclear facilities such as NPPs is accompanied by the formation and release of tritium compounds into the environment. Currently available data on the biological effects of tritium, especially its organic compounds, are very scattered and fragmentary. There is still no consensus on the regulation of the content of inorganic and organic tritium compounds in different environments. This leads, for example, to a wide range of permissible levels of tritium in the environment in different countries, which requires additional experimental and calculated data in order to harmonize standards and ensure the safety of the members of the public living near such nuclear facilities. The purpose of this work is a comparative assessment of molecular cellular biological effects (formation of DNA double-strand breaks and micronuclei) upon exposure to individual tritiated compounds in mammals.
Material and methods: An in vivo study was conducted on male rats that received orally drinking water containing tritium oxide (HTO) or 3H-thymidine with a volumetric specific activity of 800 kBq/l for 10, 21 and 31 days. The number of DNA double-strand break (DSB) repair foci in rat splenocytes was assessed by immunocytochemical staining of phosphorylated histone H2AX (γH2AX) foci. An analysis of the frequencies of polychromatophilic erythrocytes (PCE) with micronuclei (MN) in the bone marrow of rats was also carried out.
Results: The genotoxic effect of both forms of tritium was determined by a significant yield of DNA DSBs in splenocytes and micronuclei in bone marrow PCE (a more pronounced effect on the 31st day of 3H-thymidine action). With chronic exposure to 3H-thymidine on days 21 and 31, the number of γH2AX foci significantly increases; in the case of HTO, the level of foci on days 31 does not significantly change. Exposure to HTO and 3H-thymidine caused approximately the same induction of PCE with micronuclei on days 10 and 21, but by day 31 the effect of 3H-thymidine was approximately 40 % greater than that of HTO. The experiment revealed a likely genotoxic effect of inhaled tritium in control rats that were kept in the same room as the rats that received HTO and 3H-thymidine orally. However, additional experiments are needed to confirm this effect.
Conclusion: The result of this work expands the understanding of the mutation process in mammalian cells exposed to internal ionizing radiation when taking compounds containing tritium. Increased genotoxicity is observed when rats ingest drinking water containing tritium with an activity of 800 kBq/l.
Keywords: tritium, tritiated water, organically bound tritium, 3H-thymidine, γH2AX foci, DNA double-strand breaks, splenocytes, micronucleus test on polychromatophilic erythrocytes, rats
For citation: Rodneva SM, Sycheva LP, Maksimov AA, Zhorova ES, Tsishnatti AA, Tishchenko GS, Fedotov YuA, Trubchenkova TM, Yashkina EI, Guryev DV, Barchukov VG. Genotoxic Effects in Spleen and Bone Marrow of Wistar Rats Chronically Exposed to Tritium Oxide and 3H-Thymidine with Drinking Water. Medical Radiology and Radiation Safety. 2024;69(5):15–20. (In Russian). DOI:10.33266/1024-6177-2024-69-5-15-20
References
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7. Snigireva GP, Khaimovich TI, Bogomazova AN, Gorbunova IN, Nagiba VI, Nikanorova EA, et al. Cytogenetic Examination of Nuclear Specialists Exposed to Chronic Beta-Radiation of Tritium. Radiatsionnaya biologiya. Radioekologiya = Radiation Biol. Radioecol. 2009;49(1):60-6 (In Russ.).
8. Radiation Safety Standards. NRB-99/2009. SanPin 2.6.1.2523-09. Ionizing radiation, Radiation Safety. Appendix 2a.
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9. Gueguen Y, Priest ND, Dublineau I, Bannister L, Benderitter M, Durand C, et al. Vivo Animal Studies Help Achieve International Consensus on Standards and Guidelines for Health Risk Estimates for Chronic Exposure to Low Levels of Tritium in Drinking Water. Environ Mol Mutagen. 2018;59(7):586-94. DOI: 10.1002/em.22200. PMID: 30151952.
10. Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the Protection of Animals used for Scientific Purposes. ELI: http://data.europa.eu/eli/dir/2010/63/oj
11. Osipov A, Chigasova A, Yashkina E, Ignatov M, Fedotov Y, Molodtsova D, Vorobyeva N, Osipov AN. Residual Foci of DNA Damage Response Proteins in Relation to Cellular Senescence and Autophagy in X-Ray Irradiated Fibroblasts. Cells. 2023;Apr 21;12(8):1209. DOI: 10.3390/cells12081209. PMID: 37190118.
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13. OECD Guideline for the Testing of Chemicals N474. 2016. Mammalian Erythrocytes Micronucleus Test. Adopted: 29 July 2016 https://www.oecd-ilibrary.org/docserver/9789264264762-en.pdf?expires=1619012690&id=id&accname =guest&checksum=86524B6E2974E8366F62DA2CFD91BDB7
14. Guidelines. Assessment of the Mutagenic Activity of Environmental Factors in Cells of Various Mammalian Organs Using the Micronuclear Method. Moscow, Interdepartmental Scientific Council on Human Ecology and Environmental Hygiene of the Russian Federation Publ., 2001. 22 p. (In Russ.).
15. Heddle JA, Cimino MC, Hayashi M, et al. Micronuclei as an Index of Cytogenetic Damage: Past, Present, and Future. Environ Mol Mutagen. 1991;18:277-91. DOI: 10.1002/em.2850180414. PMID: 1748091.
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17. Alloni D, Cutaia C, Mariotti L, Friedland W, Ottolenghi A. Modeling Dose Deposition and DNA Damage Due to Low-Energy H3 Emitters. Radiat Res. 2014;182:322-30. DOI: 10.1667/RR13664.1.
18. Vorobyeva NYu, Kochetkov OA, Pustovalova MV, Grekhova AK, Blokhina TM, Yashkina EI, et al. Comparative Study of γH2AX Foci Formation in Human Mesenchymal Stem Cells Exposed to 3H-thymidine, Tritium Oxide and X-rays. Kletochnyye Tekhnologii v Biologii i Meditsine = Cell Technologies in Biology and Medicine. 2018;3:205-8 (In Russ.).
19. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans (78) // Ionizing Radiation. Part 2. Some Internally Deposited Radionuclides. Lyon, France: IARC Working Group on the Evaluation of Carcinogenic Risks to Humans, 2001.
20. Priest ND, Blimkie MS, Wyatt H, Bugden M, Bannister LA, Gueguen Y, et al. Tritium (3H) Retention in Mice: Administered As HTO, DTO or as 3H-Labeled Amino-Acids. Health Phys. 2017;112(5):439-44. DOI: 10.1097/HP.0000000000000637. PMID: 28350697.
21. Roch-Lefevre S, Gregoire E, Martin-Bodiot C, Flegal M, Freneau A, Blimkie M, et al. Cytogenetic Damage Analysis in Mice Chronically Exposed to Low-Dose Internal Tritium Beta-Particle Radiation. Oncotarget. 2018;9(44):27397-411. DOI: 10.18632/oncotarget.25282. PMID: 29937993.
22. Rapport IRSN 2021-00206. Actualisation Des Connaissances Sur Les Effets Biologiques Du Tritium. Clamart, France, the Institute for Radiation Protection and Nuclear Safety (IRSN), 2021. 68 p. ttps://www.irsn.fr/sites/default/files/documents/actualites_presse/ actualites/20210506_IRSN-rapport-2021-00206-TRITIUM.pdf
23. Rodneva SM, Osipov AA, Gur’ev DV, Tsishnatti AA, Fedotov YuА, Yashkina EI, Vorob’eva NYu, Maksimov AA, Kochetkov OA, Osipov AN. Comparative Investigations of the Γh2ax Foci Forming in Human Lung Fibroblasts Incubated in Media Containing Tritium-Labeled Thymidine or Amino Acids. Kletochnyye Tekhnologii v Biologii i Meditsine = Cell Technologies in Biology and Medicine. 2021;3:166-70 (In Russ.). DOI: 10.47056/1814-3490-2021-3-166-170.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The research was carried out within the framework of the state task, the research topic is «Track-1» reg. # AAAA-A19-119031190033-1.
Contribution. Rodneva S.M., Sycheva L.P., Maksimov A.A., Zhorova E.S., Guryev D.V. – preparation of the text of the article, analysis and interpretation of data, conducting experiments, collection and analysis of literary material; Zhorova E.S., Guryev D.V. – development of the concept and design of the study, internal audit; Rodneva S.M., Sycheva L.P., Maksimov A.A., Zhorova E.S., Tsishnatti A.A., Tishchenko G.S., Fedotov Yu.A., Trubchenkova T.M. a., Yashkina E.I., Guryev D.V. – conducting experiments and statistical data processing; Rodneva S.M., Sycheva L.P., Guryev D.V., Maksimov A.A., Barchukov V.G. – scientific editing of the text, verification of critically important intellectual content; Guryev D.V. – approval of the final version of the manuscript.
Article received: 20.05.2024. Accepted for publication: 25.06.2024.
Medical Radiology and Radiation Safety. 2024. Vol. 69. № 5
DOI:10.33266/1024-6177-2024-69-5-28-33
A.V. Simakov, Yu.V. Abramov, N.L. Proskuryakova, T.M. Alferova, А.К. Mayer
Methodological Approaches to Establishment
of Class of Work with Open Sources of Ionizing Radiation
A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: A.V. Simakov, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
One of the requirements of the current Basic Sanitary Rules for Radiation Safety (OSPORB-99/2010) for the organization of work with open sources of radiation (radioactive materials) is the establishment of class of work that determines the zonality of industrial premises, requirements for their decoration, layout of technological equipment, ventilation, using of personal protective equipment, etc. Formalization of such requirements is necessary as in the design of enterprises, as well as for the organization safety working conditions for personnel in existing production facilities. The accumulated long-term experience of the country’s nuclear industry has shown the validity of the requirements imposed by the existing regulatory documents for the organization of work with open nuclides at the initial stage оrganisation of large-scale production. In article presents methodological approaches to the procedure for establishing class of work as at the projected enterprises of the nuclear industry and when adjusting the established classes of work at existing enterprises. Substantiated approaches to determining the value of the minimum significant activity of a radionuclide based on data on its radiotoxicity under internal irradiation (with inhalation intake), as required according to OSPORB-99/2010. In article proposed new values of the minimum significant activity for a number of radionuclides. Unified classes of work are recommended for individual sections of separation and sublimate uranium production. The procedure for determining the actual total activity at the workplaces of uranium production is described, taking into account data on the contamination of work surfaces and the volumetric activity of uranium aerosols in the air of the working area. These methodological approaches were implemented in the Guidelines of MU 2.6.1.02-03 “Establishment of class of work with open radionuclide sources in the handling of uranium and its compounds at the enterprises of JSC “TVEL”, in the Guidelines of MU 2.6.1. 044-08 “Establishment of class of work when dealing with open sources of radiation” as well as in the draft updated Guidelines for establishing the class of work when working with open sources of ionizing radiation, developed in 2024.
Keywords: radiation safety, open sources of radiation, class of work, radionuclide, radiation hazard group, minimal significant activity
For citation: Simakov AV, Abramov YuV, Proskuryakova NL, Alferova TM, Mayer АК. Methodological Approaches to Establishment of Class of Work with Open Sources of Ionizing Radiation. Medical Radiology and Radiation Safety. 2024;69(5):28–33. (In Russian). DOI:10.33266/1024-6177-2024-69-5-28-33
References
1. SanPiN 2.6.1.2523-09. Radiation Safety Standards (NRB-99/2009) (In Russ.).
2. Standards of Radiation Safety NRB-76/87 (In Russ.).
3. MU 2.6.1.02-03. Establishment of a Class of Work with Open Radionuclide Sources When Handling Uranium and its Compounds at the Enterprises of JSC «TVEL» (In Russ.).
4. SP 2.6.1.799-99. Basic Sanitary Rules for Radiation Safety (OSPORB-99) (In Russ.).
5. SP 2.6.1.2612-10. Basic Sanitary Rules for Radiation Safety (OSPORB-99/2010) (In Russ.).
6. International Basic Safety Standards for Protection Against Ionizing Radiation and for the Safety of Radiation Sources – IAEA 1996.
7. Radiation Protection-65 “Principles and Methods for Establishing Concentration and Quantities (Exemption values) Below which Reporting is not Required in the European Directive” (Doc. XI-028/93).
8. MU 2.6.1.044-0.8 Establishment of the Class of Work when Handling Open Sources of Radiation (In Russ.).
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.05.2024. Accepted for publication: 25.06.2024.
Medical Radiology and Radiation Safety. 2024. Vol. 69. № 5
DOI:10.33266/1024-6177-2024-69-5-34-41
A.V. Titov, Iu.S. Belskikh, D.V. Isaev, N.K. Shandala,
T.A. Doroneva, Iu.V. Krotkova, M.P. Semenova, A.A. Shitova, A.A. Filonova
Radio-Ecological Situation in the Area of the Settlement of Narta (Kalmykia)
A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: A.V. Titov, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To assess radio-ecological situation in the settlement of Narta and estimate current public doses.
Material and methods: To measure the ambient dose equivalent rate (ADER), the pedestrian gamma survey method was used using a portable spectrometric complex MKS-01A “Multirad-M” and dosimeter-radiometer MKS- AT6101c. To measure the ADER in courtyards and indoors, a gamma radiation dosimeter DKG-02U “Arbiter” was used. The activity of gamma-emitting radionuclides in soil samples was measured using a stationary gamma spectrometer from CANBERRA. The activities of 210Po and 210Pb were measured using a radiometric installation UMF-2000 following their radiochemical separation from samples. Volumetric activity (VA) and equilibrium equivalent volumetric activity (EEVA) of radon were measured with an aerosol alpha radiometer for radon and thoron RAA-20P2 “Poisk”, REI-4 cameras with film track detectors of the TRACK-REI 1M set (Russia) and an integrated radon radiometer «Radon Scout PLUS». Public dose assessment was carried out in accordance with a guidelines MU 2.6.1.1088-02.
Results: The average value of the ADER on the territory of the settlement is 0.10±0.01 µSv/h, and in the premises this value is 0.10±0.02 µSv/h. Average annual values of radon VA in dwellings range from 27 to 330 Bq/m3 (average value is
110 Bq/m3, median is 97 Bq/m3). Average annual individual effective radiation doses to the population from all natural factors range from 2.5 to 13 mSv, with an average value of 5.4 and a median of 4.9 mSv.
Conclusions: The radio-ecological situation is the settlement of Narta does not differ from that in the background settlement – the village of Uldyuchiny. The average annual values of EEVA in dwellings and offices of the settlement do not exceed the permissible level for the operating buildings of 200 Bq/m3. The annual individual effective public dose in the village of Narta is higher than the average value for Kalmykia (3.4 mSv) due to increased dose values from the inhalation intake of radon and its daughter radionuclides in the premises.
Keywords: radio-ecological survey, natural radionuclides, gamma radiation, radon, mine, specific activity
For citation: Titov AV, Belskikh IuS, Isaev DV, Shandala NK, Doroneva TA, Krotkova IuV, Semenova MP, Shitova AA, Filonova AA. Radio-Ecological Situation in the Area of the Settlement of Narta (Kalmykia). Medical Radiology and Radiation Safety. 2024;69(5):34–41. (In Russian). DOI:10.33266/1024-6177-2024-69-5-34-41
References
1. URL: https://koka-lermont.livejournal.com/2820131.html. Koltsov’s Expedition (accessed date: April 22, 2024) (In Russ.).
2. Pyatov EA. Strane Byl Nuzhen Uran. Istoriya Geologorazvedochnykh Rabot na Uran v SSSR =The Country Needed Uranium. History of Geological Exploration for Uranium in the USSR. Ed. G.A.Mashkovtsev. Moscow Publ., 2005. 246 p.
(In Russ.).
3. Naseleniye Poselka Narta Priyutnenskogo Rayona Respubliki Kalmykiya = Population of the Village of Narta, Priyutnensky District, Republic of Kalmykia. URL: https://bdex.ru/naselenie/respublika-kalmykiya/n/priutnenskiy/narta/ (accessed date: April 22, 2024) (In Russ.).
4. Radiatsionnaya Obstanovka na Territorii Rossii i Sopredel’nykh Gosudarstv v 2019 godu = Radiation Situation in Russiaand Neighboring Countries in 2019. Yearbook. Obninsk Publ., 2020. 339 p. (In Russ.).
5. Åkerblom G., German O., Stamat I., Soderman Ann-Louis, Venkov V. Radon v Zhilykh Zdaniyakh Respubliki Kalmykiya. Rezul’taty Natsional’noy Programmy Issledovaniy za 2006-2007 gg. = Radon in Residential Buildings of the Republic of Kalmykia. Resultsfrom the National Radon Survey 2006-2007. Report. St. Petersburg Publ., 2009. 46 p. (In Russ.). ISSN 2000-0456. URL: www.stralsakerhetsmyndigheten.se
6. Radiatsionnaya Obstanovka na Territorii Rossiyskoy Federatsii v 2022 godu. Spravochnik = Radiation Situation on the Territory of the Russian Federation in 2022. Handbook. St. Petersburg Publ., 2023. 66 p. (In Russ.).
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was financed under the State Contract as part of the Federal Target Program «Nuclear and Radiation Safety for 2016-2020 and for the period until 2030».
Contribution. Titov A.V., Senior Researcher – data collection and processing, writing the text; Belskikh Iu.S., Researcher – data collection and processing, writing the text; Isaev D.V., Senior Researcher – data collection and processing, writing the text; Shandala N.K., Deputy General Director, Dr. Sci. Med. – study conception and design, writing and editing the text; Doroneva T.A., Researcher – sample measurements, statistical data processing; Krotko-
va Iu.V., Junior Researcher – sample measurements, statistical data processing; Semenova M.P., Senior Researcher – literary material analysis, editing the text; Shitova A.A., Junior Researcher – sample measurements, statistical data processing; Filonova A.A., Senior Researcher – sample measurements, statistical data processing All authors are responsible for approval of the final version of the article and integrity of all parts of the article.
Article received: 20.05.2024. Accepted for publication: 25.06.2024.
Medical Radiology and Radiation Safety. 2024. Vol. 69. № 5
DOI:10.33266/1024-6177-2024-69-5-21-27
L.A. Romodin, А.А. Moskovskij
Assessment of the Effect of Ascorbic, Malic and Succinic Acids
on Radiation-Induced Oxidative Stress in A549 Cells
A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: L.A. Romodin, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Relevance: For modern radiobiology, the problem of finding pharmacological protection against radiation damage remains urgent. Interest in this topic does not weaken due to the high chemical toxicity of all generally recognized radioprotectors. One of the most studied drugs in this regard are substances with antioxidant activity, which is due to the ability of antioxidants to inhibit the processes of oxidative stress.
Purpose: The effect of malic, succinic and ascorbic acids on radiation-induced oxidative stress in the culture of human lung adenocarcinoma cells of the A549 line.
Material and methods: In the course of the work, the effect of solutions of malic, ascorbic and succinic acids in concentrations of 0.1, 0.5, 1 and 2 mM on the intensity of radiation-induced oxidant stress in the adsorption culture of cells of the A549 line was studied. Oxidative stress was induced by X-ray radiation at a dose of 8 Gy. The level of reactive oxygen species was estimated based on the ratio of the fluorescence intensity of the dye dichlorofluorescein to that of the dye Hoechst-33342.
Results: Under the influence of the studied substances, a statistically significant decrease in the content of reactive oxygen species in the cells was observed. The most pronounced effect is observed in samples treated with succinic acid. In non-irradiated samples in the presence of ascorbic and malic acids at a concentration of the studied substances of 100 mM, a statistically significant increase in the intensity of fluorescence is observed, which can be explained by the reduction of intracellular trivalent iron to Fe2+ under the action of these substances, which contributed to the Fenton reaction.
Conclusions: Based on the results obtained during this study, it can be assumed that malic acid, ascorbic acid and, in particular, succinic acid have some radioprotective properties. However, additional studies on other model systems, including various cell lines, are needed to confirm the presence of these properties. The results of the presented work make it possible in the future to begin the development of therapeutic schemes to alleviate the effects of radiation using the studied substances.
Keywords: A549 cells, X-rays, oxidative stress, malic acid, ascorbic acid, succinic acid
For citation: Romodin LA, Moskovskij АА. Assessment of the Effect of Ascorbic, Malic and Succinic Acids on Radiation-Induced Oxidative Stress in A549 Cells. Medical Radiology and Radiation Safety. 2024;69(5):21–27. (In Russian). DOI:10.33266/1024-6177-2024-69-5-21-27
References
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was carried out within the framework of research and development «Technology-3» (the registration number of research and development in the EGISU R&D system: 1230113001053).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.05.2024. Accepted for publication: 25.06.2024.
Medical Radiology and Radiation Safety. 2024. Vol. 69. № 5
DOI:10.33266/1024-6177-2024-69-5-42-52
V.V. Vostrotin
Using the Bayesian Approach for the Case of Acute Inhalation of Pu-239 Industrial Compounds
Southern Urals Biophysics Institute, Ozyorsk, Russia
Contact person: V.V. Vostrotin, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Introduction: The Bayesian approach has found wide application for the tasks of estimating doses of internal exposure under various intake scenarios. The South Ural Institute of Biophysics has accumulated considerable experience in using the Bayesian approach to estimate the expected effective doses of internal exposure for current individual dosimetry control when radionuclides intake into body by various ways. Attributing the type of compounds Moderate or Slow according to the NRB-99/2009 classification for acute inhalation of industrial Pu-239 compounds bias dose estimates to the lungs, which leads to the need to develop a new methodology.
Purpose: The development of a calculation methodology using the Bayesian approach for the case of acute inhalation of industrial Pu-239 compounds into the human body and its testing in artificial cases.
Material and methods: A technique is presented for interpreting a series of measurement results of Pu-239 activity in daily urine and/or daily feces to assess the distribution of intake, two key parameters of the biokinetic model of ICRP Publication 66 (the proportion of rapid absorption fr and the rate of slow absorption into the blood ss), as well as annual weighted equivalent doses to the lungs. The technique allows using a prior information about the estimated parameters and correctly processing measurement results below the detection limit.
Results: A jDose program has been created that implements the technique in ~ 20 minutes on a modern office computer. The program was tested on artificial cases with 10 measurements of Pu-239 activity in daily urine and 10 in daily feces during the first 10 days after acute inhalation intake at AMAD = 1 µm. Testing showed reproducibility of the set «true» parameter values in the range (average ± 2 standard deviations) with an increase in the proportion of unreliable measurement results. The increase in the proportion of unreliable measurement results had the greatest impact on the estimation of the coefficient of variation of the slow absorption rate parameter into the blood ss.
Keywords: plutonium, inhalation intake, internal exposure, Bayesian approach
For citation: Vostrotin VV. Using the Bayesian Approach for the Case of Acute Inhalation of Pu-239 Industrial Compounds. Medical Radiology and Radiation Safety. 2024;69(5):42–52. (In Russian). DOI:10.33266/1024-6177-2024-69-5-42-52
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The author declares that there are no conflicts of interest.
Financing. The work was carried out within the framework of the research project « Improvement of control methods and study of the peculiarities of the formation of internal radiation doses for the personnel of Mayak PA and the population of adjacent territories», cipher «Control-22», funded by the FMBA of Russia.
Contribution. Conceptual development, creation of R scripts, mathematical calculations and their quality control were carried out by one author.
Article received: 20.05.2024. Accepted for publication: 25.06.2024.