JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.
Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.
The journal is included in the List of Russian Reviewed Scientific Journals of the Higher Attestation Commission. Since 2008 the journal has been available on the Internet and indexed in the RISC database which is placed on Web of Science. Since February 2nd, 2018, the journal "Medical Radiology and Radiation Safety" has been indexed in the SCOPUS abstract and citation database.
Brief electronic versions of the Journal have been publicly available since 2005 on the website of the Medical Radiology and Radiation Safety Journal: http://www.medradiol.ru. Since 2011, all issues of the journal as a whole are publicly available, and since 2016 - full-text versions of scientific articles. Since 2005, subscribers can purchase full versions of other articles of any issue only through the National Electronic Library. The editor of the Medical Radiology and Radiation Safety Journal in accordance with the National Electronic Library agreement has been providing the Library with all its production since 2005 until now.
The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.
Since 2017 the journal Medical Radiology and Radiation Safety has switched to digital identification of publications, assigning to each article the identifier of the digital object (DOI), which greatly accelerated the search for the location of the article on the Internet. In future it is planned to publish the English-language version of the journal Medical Radiology and Radiation Safety for its development. In order to obtain information about the publication activity of the journal in March 2015, a counter of readers' references to the materials posted on the site from 2005 to the present which is placed on the journal's website. During 2015 - 2016 years on average there were no more than 100-170 handlings per day. Publication of a number of articles, as well as electronic versions of profile monographs and collections in the public domain, dramatically increased the number of handlings to the journal's website to 500 - 800 per day, and the total number of visits to the site at the end of 2017 was more than 230.000.
The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Issues journals
Medical Radiology and Radiation Safety. 2025. Vol. 70. № 2
DOI:10.33266/1024-6177-2025-70-2-16-22
S.A. Abdullaev1, 2, 3, D.V. Fomina1, 3, V.O. Menukhov1, 2, M.V. Dushenko1,
A.V. Tochilenko4, T.P. Kalinin5, E.V. Evdokimovskii2
Changes in the Copy Number and Gene Expression of mtDNA
in Various Tissues of Mice Exposed to Local Brain Irradiation
1 N.N. Semenov Federal Research Center for Chemical Physics, Moscow, Russia
2 Institute of Theoretical and Experimental Biophysics, Pushchino, Russia
3 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
4 National Research Nuclear University MEPhI, Moscow, Russia
5 N.I. Pirogov Russian National Research Medical University, Moscow, Russia
Contact person: S.A. Abdullaev, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To determine changes in the copy number and gene expression of mtDNA in various tissues of mice subjected to local irradiation of the brain.
Material and methods: Male Balb/c mice aged 2 months were used. Only the head of the mouse was exposed to X-ray irradiation at a dose of 5 Gy (power 2.5 Gy/min). After removal from the animals, the tissues were homogenized on ice, after which the homogenate mass was divided into two parts to isolate nucleic acids. Liquid blood was collected separately, after which nucleated blood cells were separated into granulocyte and monocyte fractions by differential centrifugation by Diacoll density gradient. The total number of mtDNA copies and gene expression were assessed using real-time PCR.
Results: It was shown that in nucleated blood cells, after irradiation, the relative number of transcripts of the mitochondrial gene ATP6 increases. In granulocytes, this effect is much more pronounced than in monocytes. At the same time, the amount of mitochondrial DNA in nucleated blood cells decreases relative to the control level by 2–3 times. In the brain exposed to irradiation, an increase in the relative amount of mtDNA transcripts by about 3 times is also observed, compared to the control. In organs not exposed to irradiation (heart, liver, spleen), the same effect is observed as in the brain, namely, an increase in the relative amount of mtDNA transcripts. The number of copies of mtDNA itself in brain cells, after a sharp increase a day after irradiation, sharply decreases and remains so until the end of the experiment, 30 days later. In liver and heart cells, the opposite process occurs, namely, a significant increase in the number of mtDNA copies, with a maximum at 14–21 days from the moment of irradiation.
Conclusion: Thus, the obtained results allow us to say that the observed changes are due to the occurrence of the “bystander effect” that arose after local irradiation of the brain with X-ray radiation at a dose of 5 Gy.
Keywords: mtDNA, bystander effect, oxidative stress, X-ray exposure, brain, mice
For citation: Abdullaev SA, Fomina DV, Menukhov VO, Dushenko MV, Tochilenko AV, Kalinin TP, Evdokimovskii EV. Changes in the Copy Number and Gene Expression of mtdna in Various Tissues of Mice Exposed to Local Brain Irradiation. Medical Radiology and Radiation Safety. 2025;70(2):16–22. (In Russian). DOI:10.33266/1024-6177-2025-70-2-16-22
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was supported by the Russian Science Foundation (project No. 24-24-00446).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.12.2024. Accepted for publication: 25.01.2025.
Medical Radiology and Radiation Safety. 2025. Vol. 70. № 2
DOI:10.33266/1024-6177-2025-70-2-23-26
Yu.A. Zrilova1, A.K. Chigasova1, 2, 3, M.A. Ignatov1, 2, N.Yu. Vorobyеva1, 2,
A.A. Osipov2, V.O. Saburov4, E.I. Kazakov4, S.N. Koryakin4,
Yu.A. Fedotov1, 2, A.Yu. Bushmanov1, A.N. Osipov1, 2
Low Efficiency of DNA Repair in Human Peripheral Blood Lymphocytes Irradiated ex vivo by 14.1 MeV Neutrons
1 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2 N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, Moscow, Russia
3 Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow, Russia
4 A.F. Tsyb Medical Radiological Research Center – branch of the National Medical Research
Radiological Centre of the Ministry of Health of the Russian Federation, Obninsk
Contact person: A.N. Osipov, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract
Purpose: To evaluate the efficiency of DNA repair in human peripheral blood lymphocytes irradiated ex vivo by 14.1 MeV neutrons.
Material and methods: The peripheral blood of three physically healthy male donors aged 28–40 years was used for the study. The peripheral blood was collected in K2EDTA vacutainers (Vacuette). All donors gave their consent to conduct this study. Isolation of lymphocytes was performed by centrifugation in a ficoll-verografin density gradient of 1.077 g/cm3 (Histopaque, Sigma-Aldrich) in accordance with the attached instructions. Cell irradiation was performed at the A.F. Tsyb Medical Radiological Research Center on the NG-14 neutron generator (FSUE VNIIA, Russia), which provided neutron fluxes with an energy of 14.1 MeV, and the gamma-therapeutic device “ROKUS-AM” (JSC Ravenstvo, Russia; cobalt-60, dose rate 0.5 Gy/min) at doses of 0.1, 0.25 and 0.5 Gy. To assess the effectiveness of DNA repair, the DNA comet method was used under alkaline conditions. The study was performed immediately after irradiation and after 15 min of cell incubation in complete culture medium at 37 °C. The tail moment and % DNA in the comet tail were used as a criterion for DNA damage. Statistical significance was assessed using analysis of variance (ANOVA).
Results: It was demonstrated that the efficiency of DNA repair in lymphocytes peripheral blood of a person after exposure to 14.1 MeV neutrons is ~ 4–5 times lower than after exposure to cobalt-60 gamma-radiation. The results obtained indicate that in the case of exposure to 14.1 MeV neutron radiation, the contribution of complex, difficult-to-repair DNA damage is much higher than when exposed to gamma radiation, which determines the high relative biological effectiveness of neutron radiation.
Keywords: fast neutrons, lymphocytes, DNA damage, DNA repair, DNA comet assay
For citation: Zrilova YuA, Chigasova AK, Ignatov MA, Vorobyеva NYu, Osipov AA, Saburov VO, Kazakov EI, Koryakin SN, Fedotov YuA, Bushmanov AYu, Osipov AN. Low Efficiency of DNA Repair in Human Peripheral Blood Lymphocytes Irradiated ex vivo by 14.1 MeV Neutrons. Medical Radiology and Radiation Safety. 2025;70(2):23–26. (In Russian). DOI:10.33266/1024-6177-2025-70-2-23-26
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The research was carried out within the framework of the state assignment of the Ministry of Science and Higher Education of the Russian Federation (No. 1023112000035-8, code “Cosmos-DNA”).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.12.2024. Accepted for publication: 25.01.2025.
Medical Radiology and Radiation Safety. 2025. Vol. 70. № 2
DOI:10.33266/1024-6177-2025-70-2-35-39
M.V. Pustovalova1, V.D. Nekrasov1, E.V. Andreev3, 4, I.N. Fadeikina3, 4,
S.V. Leonov1, A.N. Nechaev3, 4, A.N. Osipov1
Synthesized Using β-Cyclodextrin Silver and Gold Nanoparticles
as Radiosensitizers in Breast Cancer Radiotherapy
1 Moscow Institute of Physics and Technology, Dolgoprudny, Russia
2 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
3 Joint Institute for Nuclear Research, Dubna, Russia
4 Dubna State University, Dubna, Russia
Contact person: A.N. Osipov, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Abstract
Purpose: 1) Synthesis and characterization of β-cyclodextrin stabilized silver and gold nanoparticles. 2) Evaluation of the effect of the synthesized nanoparticles on the severity of radiobiological effects in irradiated breast cancer (BC) cells.
Material and methods: Gold and silver nanoparticles were synthesized using β-cyclodextrin as a reducing agent and stabilizer. Human BC cell lines MDA-MB-231 (ER-/PR-/ EGFR+/ HER2-) and MCF7 (ER+/ PR+/ EGFR-/ HER2-) were used in the work. The cells were irradiated on a RUB RUST-M1 (Russia) X-ray irradiation facility, equipped with two X-ray emitters, at a dose rate of 0.85 mGy/min, 200 kV voltage, 5.0 mA current, 1.5 mm Al filter. Nanoparticles were added 24 h before irradiation at a concentration of 0.5 mg/l. Cells without nanoparticles were used as a control. To assess radiobiological effects, foci of the DNA damage marker protein (γH2AX) were analyzed 1 and 24 hours after irradiation. Statistical and mathematical data analysis was performed using GraphPad Prism 9.0.2.161 software (GraphPad Software). Statistical significance was assessed using analysis of variance (ANOVA).
Results: Effective radiosensitization of BC cell lines MDA-MB-231 and MCF7 using β-cyclodextrin stabilized gold and silver nanoparticles (AuNPs and AgNPs) was shown. The results obtained indicate the achievement of statistically significant results already at a concentration of 0.5 mg/l, which is at least 20 times less than the concentrations previously used to achieve a significant effect.
Keywords: breast cancer, radiation therapy, radiosensitizers, silver nanoparticles, gold nanoparticles, β-cyclodextrin
For citation: Pustovalova MV, Nekrasov VD, Andreev EV, Fadeikina IN, Leonov SV, Nechaev AN, Osipov AN. Synthesized Using β-Cyclodextrin Silver and Gold Nanoparticles as Radiosensitizers in Breast Cancer Radiotherapy. Medical Radiology and Radiation Safety. 2025;70(2):35–39. (In Russian). DOI:10.33266/1024-6177-2025-70-2-35-39
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. Radiobiological research was carried out with the support of the Russian Science Foundation (project No. 24-45-20002, https://rscf.ru/project/24-45-20002 /). The synthesis and characterization of nanoparticles were performed within the framework of the state assignment of the Ministry of Science and Higher Education of the Russian Federation (No. 1024011000011-7-1.4.2;3.5.2 Conjugates of boron-containing quantum dots with biovectors for the diagnosis and boron-neutron capture therapy of superficial malignant tumors (FEEM-2024-0011)).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.12.2024. Accepted for publication: 25.01.2025.
Medical Radiology and Radiation Safety. 2025. Vol. 70. № 2
DOI:10.33266/1024-6177-2025-70-2-27-34
P.A. Malakhov1, V.V. Maximov2, M.V. Pustovalova1, A.V. Smirnova1, Z. Nofal1,
V. Saburov3, A.N. Osipov1, D.V. Kuzmin1, S.V. Leonov1, 4
MiR-16-1-3p and miR-16-2-3p Overexpression Confers Tumor Suppressive and Antimetastatic Properties in Radioresistant A549 Non-Small Cell Lung Cancer Cells
1 Institute of Future Biophysics, Dolgoprudny, Russia
2 Department of Molecular Genetics and Microbiology, Institute of Medical Research, Israel–Canada,
Faculty of Medicine, Hebrew University, Jerusalem, Israel
3 A.F. Tsyb Medical Research Center of Radiology, Obninsk, Russia
4 Pushchino Scientific Center for Biological Research Institute of Cell Biophysics, Pushchino, Russia
Contact person: M.V. Pustovalova, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: Lung cancer is the leading cause of death worldwide, with non-small cell lung cancer (NSCLC) accounting for 85 % of all lung cancers. Combined chemoradiotherapy is one of options in the treatment of patients with inoperable NSCLC. However, the prognosis of NSCLC remains unsatisfactory due to the development of radio- and chemo-resistance of cancer cells. This study aimed to investigate how the overexpression of miR-16, miR-16-1-3p, and miR-16-2-3p influences clonogenic survival, migration, and sensitivity to cisplatin in both radiosensitive and radioresistant non-small cell lung cancer (NSCLC) cells.
Material and methods: This study involved the application of single proton beam irradiation to A549 NSCLC cells, resulting in the emergence of a subline of resilient radioresistant daughter cells, designated as A549IR. To explore the functional role of the miR-16, miR-16-1-3p, and miR-16-2-3p in NSCLC, we overexpressed the “leader” miR-16 as well as the “passenger” miR-16-1-3p and miR-16-2-3p strands in both the parental A549 and their radioresistant variant, A549IR cells. The impact of microRNA overexpression on cell viability was evaluated through a clonogenic assay. Additionally, cisplatin sensitivity was measured by calculating the total mass of surviving cells via the sulforhodamine B method. Furthermore, the capacity for cell migration and invasion was investigated using Boyden chambers.
Results: Overexpressing miR-16, miR-16-1-3p, and miR-16-2-3p significantly reduced the ability of A549 and radioresistant A549IR NSCLC cells to survive, clone, migrate, and invade, compared to cells with normal levels of these microRNAs. Moreover, the stable overexpression of these microRNAs markedly enhanced the sensitivity of A549 and A549IR cells to the cytotoxic effects of cisplatin, allowing for a nearly threefold reduction in the concentration needed to achieve 50 % cell death.
Conclusion: An increase in the expression of “passenger” miR-16-1-3p and miR-16-2-3p, as well as the “leader” miR-16, exhibits a robust tumor-suppressive and cisplatin-sensitizing activities in both the radiation-sensitive parental and the radiation-resistant daughter cells in the human NSCLC A549 lineage.
Keywords: non-small cell lung cancer, chemo-radiotherapy, radioresistance, metastasis, invasiveness, miR-16, miR-16-1; miR-16-2
For citation: Malakhov PA, Maximov VV, Pustovalova MV, Smirnova AV, Nofal Z, Saburov V, Osipov AN, Kuzmin DV, Leonov SV. MiR-16-1-3p and miR-16-2-3p Overexpression Confers Tumor Suppressive and Antimetastatic Properties in Radioresistant A549 Non-Small Cell Lung Cancer Cells. Medical Radiology and Radiation Safety. 2025;70(2):27–34. (In Russian). DOI:10.33266/1024-6177-2025-70-2-27-34
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was supported by Russian Science Foundation (agreement No 23-14-00220).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.12.2024. Accepted for publication: 25.01.2025.
Medical Radiology and Radiation Safety. 2025. Vol. 70. № 2
DOI:10.33266/1024-6177-2025-70-2-40-48
P.S. Miklyaev1, 2, T.B. Petrova3, P.A. Sidyakin4, A.M. Marennyy2,
R.A. Tekeev5, A.A. Tsapalov6, D.V. Shchitov4, D.N. Tsebro4,
M.A. Murzabekov4, L.E. Karl2, S.G. Gavriliev1
Formation of Radon Situation in Buildings of Educational Institutions of Lermontov Town
1 E.M. Sergeev Institute of Environmental Geoscience, Moscow, Russia
2 Research and Technical Center of Radiation-Chemical Safety and Hygiene, Moscow, Russia
3 M.V. Lomonosov Moscow State University, Moscow, Russia
4 North Caucasus Federal University, Stavropol, Russia
5 Center of Hygiene and Epidemiology № 101, Lermontov, Russia
6 Scientific Production Company «Doza», Zelenograd, Moscow, Russia
Contact person: V.A. Manukova, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To determine the levels of indoor radon EEC of educational institutions in Lermontov city, to estimate the doses due to radon, to establish the causes of increased radon activity concentration in the indoor air and the patterns of its change.
Material and methods: measurements of radon EEC in cold and warm seasons were performed using the SSNTD (TRACK-REI_1M equipment), the content of natural radionuclides in building materials and soils using the Progress-2000 scintillation gamma spectrometer based on NaI(Tl) detector, the radon exhalation rate, using the Camera-01 charcoal complex, and radon continuous monitoring was carried out with the Radon Eye and Radex MR107. A total of 19 buildings were surveyed.
Results: It was found that the territory is characterized by a high geogenic radon, the average radium-226 content is 103 Bq/kg (range from 45 to 230 Bq/kg), the average value of radon exhalation is 263 mBq/(m2s) (range from 31 to 2730 mBk/(m2s)). The indoor radon EEC values of the 1st floors of educational institutions ranged from 12 to 1347 Bq/m3. Exceeding the permissible levels was found in all surveyed buildings, except for one kindergarten, where basement reliably protect against radon entering buildings. There are no clear seasonal patterns in the distribution of indoor radon, as well as a significant decrease in radon concentrations on the upper floors of buildings, which is apparently explained by intense convective mixing of air between floors. The main source of radon in buildings is its release from the soil. The entry of radon into buildings is caused by the convective transfer of radon through leaky tie-ins of communications and other defects in basement. The ineffectiveness of the radon mitigation measures is highlighted in all buildings where they were carried out. This is due to the inefficient allocation of responsibilities and the lack of competence and experience of the construction organisations carrying out this work.
Keywords: EEC, dose assessment, radon exhalation rate, soil radium content, radon monitoring, Lermontov city
For citation: Miklyaev PS, Petrova TB, Sidyakin PA, Marennyy AM, Tekeev RA, Tsapalov AA, Shchitov DV, Tsebro DN, Murzabe-
kov MA, Karl LE, Gavriliev SG. Formation of Radon Situation in Buildings of Educational Institutions of Lermontov Town. Medical Radiology and Radiation Safety. 2025;70(2):40–48. (In Russian). DOI:10.33266/1024-6177-2025-70-2-40-48
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was supported by the Russian Science Foundation grant No. 24-27-00028.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.12.2024. Accepted for publication: 25.01.2025.