SCIENTIFIC AND INFORMATION-ANALYTICAL MAGAZINE

ISSN 1024-6177 (Print), ISSN 2618-9615 (Online)

JOURNAL DESCRIPTION

The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.

Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.

Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.

The journal is included in the List of Russian Reviewed Scientific Journals of the Higher Attestation Commission. Since 2008 the journal has been available on the Internet and indexed in the RISC database which is placed on Web of Science. Since February 2nd, 2018, the journal "Medical Radiology and Radiation Safety" has been indexed in the SCOPUS abstract and citation database.

Brief electronic versions of the Journal have been publicly available since 2005 on the website of the Medical Radiology and Radiation Safety Journal: http://www.medradiol.ru. Since 2011, all issues of the journal as a whole are publicly available, and since 2016 - full-text versions of scientific articles. Since 2005, subscribers can purchase full versions of other articles of any issue only through the National Electronic Library. The editor of the Medical Radiology and Radiation Safety Journal in accordance with the National Electronic Library agreement has been providing the Library with all its production since 2005 until now.

The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.

Since 2017 the journal Medical Radiology and Radiation Safety has switched to digital identification of publications, assigning to each article the identifier of the digital object (DOI), which greatly accelerated the search for the location of the article on the Internet. In future it is planned to publish the English-language version of the journal Medical Radiology and Radiation Safety for its development. In order to obtain information about the publication activity of the journal in March 2015, a counter of readers' references to the materials posted on the site from 2005 to the present which is placed on the journal's website. During 2015 - 2016 years on average there were no more than 100-170 handlings per day. Publication of a number of articles, as well as electronic versions of profile monographs and collections in the public domain, dramatically increased the number of handlings to the journal's website to 500 - 800 per day, and the total number of visits to the site at the end of 2017 was more than 230.000.

The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.

Issues journals

Medical Radiology and Radiation Safety. 2018. Vol. 63. No. 1. P. 35-39

RADIATION BIOLOGY

DOI: 10.12737/article_5a82eb9a9bbac1.67972336

Investigation of the Influence of the Conditioning Medium Factors Obtained During the Cultivation of Bone Marrow Mesenchymal Stem Cells on the Course of Severe Local Radiation Injuries of Skin in Rats

A.A. Temnov1, T.A. Astrelina1, K.A. Rogov2, V.G. Lebedev1, T.A. Nasonova1, A.V. Lyrschikova1, Y.B. Deshevoy1, O.A. Dobrynina1, A.V. Melerzanov3, A.S. Samoylov1, A.Yu. Bushmanov1, B.B. Moroz1

1. A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. ; 2. Research Institute of Human Morphology; Moscow, Russia; 3. Moscow Institute of Physics and Technology, Dolgoprudny, Moscow region, Russia

A.A. Temnov - Leading Researcher, Dr. Sc. Med.; T.A. Astrelina - Head of Center for Biomedical Technologies, Dr. Sc. Med.; K.A. Rogov - Leading Researcher, Dr. Sc. Med., Prof.; V.G. Lebedev - Leading Researcher, PhD Biol.; T.A. Nasonova - Leading Researcher, PhD Med.; Yu.B. Deshevoy - Leading Researcher, PhD Med.; A.V. Lyrschikova - Senior Researcher, PhD Biol.; O.A. Dobrynina - Research Worker; A.V. Melerzanov - Head of Cells Center of MPhTI, PhD Med.; A.S. Samoylov - Director General, Dr. Sc. Med.; A.Yu. Bushmanov - First Deputy Director General, Dr. Sc. Med., Prof.; B.B. Moroz - Head of Lab., Academician of RAS

Abstract

Purpose: Study of the effect of paracrine factors, produced by MMSC of bone marrow during the cultivation, on the severity of local radiation injuries in the conditions of application in the early periods after irradiation.

Material and methods: Experiments were performed on rats of the breed Wistar weighing 280 g. Rats were exposed locally in iliolumbar region of the back using X-ray machine LNC-268 (RAP 100-10) at a dose of 110 Gy (30 kV tube voltage, current 6.1 mA, filter Al 0.1 mm thick), dose rate is 21.4 Gy/min. Area of the irradiation field was 8.2-8.5 cm2. The conditioned medium obtained by culturing MMSC of rats’ bone marrow was administered in dose 1.0 ml (total protein 8 mg/ml) at 1, 3, 6, 8 and 10 days after irradiation. The severity of radiation damage to the skin and the effects of therapy were evaluated in dynamics by clinical manifestations, using planimetry and histological methods.

Results: It was shown that in control animals and in rats, with the introduction of the conditioned medium, the values of the skin lesion area in the period up to the 29th day after irradiation practically did not differ, gradually decreasing in control animals from 5.9 ± 0.6 cm2 to 2.2 ± 0.3 cm2 at the 15th and 29th days after irradiation, respectively. Then, in the control group, the lesion area ranged from 1.4 ± 0.6 cm2 on the 50th day to 1.9 ± 0.8 cm2 on the 71st day. In the experimental group of animals, with the introduction of factors of the conditioning medium, a decrease in the area of the lesion and a stable dynamics of healing of radiation ulcers, beginning from the 36th day, there was a gradual decrease in the area of the lesion, which reached 0.2 ± 0.1 cm2 by the 71st day after irradiation. On the 64-71th day after irradiation, the difference between the areas of skin lesion in the experimental and control groups was statistically significant, p <0.05. The histological analysis showed that the use of paracrine factors obtained from MMSC in the process of cultivation significantly reduces the severity of the inflammatory reaction and accelerates the regeneration processes.

Conclusion: Thus, the introduction of conditioned medium factors obtained during the cultivation of mesenchymal stem cells of the bone marrow facilitates a more easy flow of the pathological process and the healing of radiation ulcers after local radiation damage to the skin of rats. Apparently, the favorable effect of paracrine factors introduced in the early periods after irradiation, with severe local radiation injuries, is associated with their effect on pathological processes in the inflammatory-destructive stage.

Key words: paracrine factors, conditioned medium, cell technology, local radiation injuries, mesenchymal stem cells, bone marrow, radiation skin ulcers, rats

REFERENCES

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For citation: Temnov AA, Astrelina TA, Rogov ˀ, Lebedev VG, Nasonova TA, Lyrschikova AV, Deshevoy YB, OA Dobrynina, Melerzanov AV, Samoylov AS, Bushmanov AYu, Moroz BB. Investigation of the Influence of the Conditioning Medium Factors Obtained During the Cultivation of Bone Marrow Mesenchymal Stem Cells on the Course of Severe Local Radiation Injuries of Skin in Rats. Medical Radiology and Radiation Safety. 2018;63(1):35-9. DOI: 10.12737/article_5a82eb9a9bbac1.67972336. Russian.

PDF (RUS) Full-text article (in Russian)

Medical Radiology and Radiation Safety. 2018. Vol. 63. No. 1. P. 28-34

RADIATION BIOLOGY

DOI: 10.12737/article_5a855c9d5b1211.49546901

3H-Thymidine Influence on DNA Double Strand Breaks Induction in Cultured Human Mesenchymal Stem Cells

N.Yu. Vorobyeva, V.V. Uyba, O.A. Kochetkov, T.A. Astrelina, M.V. Pustovalova, A.K. Grekhova, T.M. Blokhina, E.I. Yashkina, D.I. Kabanov, V.A. Nikitina, Yu.B. Suchkova, I.V. Kobzeva, A.N. Osipov

A.I. Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

N.Yu. Vorobyeva – Senior Researcher, PhD Biol.; V.V. Uyba – Head of the Federal Medical Biological Agency of Russia, Dr. Sc. Med., Prof.; O.A. Kochetkov – Head of Lab., PhD Tech.; T.A. Astrelina – Head of Center for Biomedical Technologies, Dr. Sc. Med.; M.V. Pustovalova – Research Fellow; A.K. Grekhova – Junior Researcher; T.M. Blokhina – Research Fellow; E.I. Yashkina – Junior Researcher; D.I. Kabanov – Engineer; V.A. Nikitina – Leading Researcher, PhD Med.; Yu.B. Suchkova – Senior Researcher, PhD Med.; I.V. Kobzeva – Senior Researcher, PhD Med.; A.N. Osipov – Head of Dep., Dr. Biol. Sc., Prof.

Abstract

Purpose: To estimate the impact of 3H-thymidine on DNA double strand breaks (DSBs) induction in cultured human mesenchymal stem cells (MSC).

Material and methods: Isolation and cultivation of human bone marrow MSC was carried out according to a standard procedure. A sterile solution of 3H-thymidine with different specific radioactivity was added to the cell culture and incubated under the conditions of the CO2 incubator for 24 hours. The specific radioactivity of 3H-thymidine in the incubation medium was 50–1600 kBq/ml. To evaluate quantitatively the DSBs, an immunocytochemical analysis of the DSB marker – phosphorylated histone (γH2AX) foci was used. Additionally, the proportion of dividing cells was estimated using an immunocytochemical analysis of the cell proliferation marker, the Ki67 protein.

Results: It was shown that 24 h incubation of human MSC in a culture medium results in a dose-dependent increase in γH2AX foci. There is a linear increase in the foci γH2AX in the range of 50–400 kBq/ml, after which the relative quantitative yield of foci per unit of specific radioactivity begins to decrease. In general, the dose-effect relationship is approximated by the quadratic function y = 3.13 + 50.80x – 12.38x2 (R2 = 0.99), where y is the number of foci γH2AX in the cell nucleus, and x is the specific radioactivity in 1000 kBq/ml. It was found that incubation of human MSC in a culture medium containing 800 and 1600 kBq/ml of 3H-thymidine resulted in a statistically significant decrease in the cells proliferative activity compared to the control of ~1.25 and 1.41 respectively. The peculiar biological limitation of tritium accumulation in the cell nucleus explains well the nonlinear character of the dependence of the formation of DSBs on the specific radioactivity of 3H-thymidine in the culture medium observed in our study.

Conclusion: Quantitative analysis of γH2AX foci has proved to be a highly reproducible and highly sensitive method for evaluating the induction of DSBs in living cells under the action of 3H-thymidine. An analysis of the foci of γH2AX will be useful for accurate estimating the quantitative yield of DBS in living cells per dose of 3H-thymidine β-radiation. To do this, it is necessary to make a correct calculation of the doses received by the cells taking into account the microdistribution of 3H-thymidine in the cell volume and its accumulation in the DNA of living cells.

Key words: 3H-thymidine, DNA double strand breaks, γH2AX foci, mesenchymal stem cells

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For citation: Vorobyeva NYu, Uyba VV, Kochetkov OA, Astrelina TA, Pustovalova MV, Grekhova AK, Blokhina TM, Yashkina EI, Kabanov DI, Nikitina VA, Suchkova YuB, Kobzeva IV, Osipov AN. 3H-Thymidine Influence on DNA Double Strand Breaks Induction in Cultured Human Mesenchymal Stem Cells. Medical Radiology and Radiation Safety. 2018;63(1):28-34. DOI: 10.12737/article_5a855c9d5b1211.49546901. Russian.

PDF (RUS) Full-text article (in Russian)

Medical Radiology and Radiation Safety. 2015. Vol. 60. No. 1. P. 72-74

MEDICAL PRACTICE ISSUE

B.A. Kukhta, N.N. Kononykina, V.I. Krasnyuk, A.A. Molokanov, A.G. Tsovyanov, V.N. Yatsenko, P.P. Gantsovsky, E.O. Granovskaya, O.A. Kasymova, A.S. Kretov

Assessment of the Dose and the Occupational Suitability in Case of Ingestion Intake of Cesium-137

A.I. Burnasyan Federal Medical Biophysical Center of FMBA, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

ABSTRACT

The results of different bioassays (measurements of 137Cs content in the body using whole-body counter and in excreta samples) in the case of ingestion intake of cesium and strontium at workplace are presented. Intake and committed effective dose have been assessed. Coincidence of the dose estimates based on measurements of 137Cs content in the body and in urine samples has been demonstrated. It’s concluded that the limit values of the basic standardized parameters were not exceeded.

Key words: cesium-137, ingestion intake, whole-body counter, bioassay, internal doses, basic dose limits

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For citation: Kukhta BA, Kononykina NN, Krasnyuk VI, Molokanov AA, Tsovyanov AG, Yatsenko VN, Gantsovsky PP, Granovskaya EO, Kasymova OA, Kretov AS. Assessment of the Dose and the Occupational Suitability in Case of Ingestion Intake of Cesium-137. Medical Radiology and Radiation Safety. 2015;60(1):72-4. Russian.

PDF (RUS) Full-text article (in Russian)

Medical Radiology and Radiation Safety. 2018. Vol. 63. No. 1. P. 5-27

RADIATION BIOLOGY

DOI: 10.12737/article_5a82e4a3908213.56647014

The Relationship Between the Age of the Based Laboratory Animals (Mice, Rats, Hamsters and Dogs) and the Age of Human: Actuality for the Age-Ralated Radiosensitivity Problem and the Analysis of Published Data

A.N. Koterov1, L.N. Ushenkova1, E.S. Zubenkova1, A.A. Wainson1,2, A.P. Biryukov1

1. A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. ; 2. N.N. Blokhin National Medical Research Center of Oncology, Moscow, Russia

A.N. Koterov - Head of Laboratory, Dr. Sc. Biol.; L.N. Ushenkova - Leading Researcher, PhD Biol.; E.S. Zubenkova - Leading Researcher, PhD Biol.; A.A. Wainson - Head of Group, Dr. Sc. Biol.; Prof.; A.P. Biryukov - Head of Department, Dr. Sc. Med., Prof.

Abstract

Purpose: Survey-synthetic study of published scientific data on the relationship between the ages of the most used laboratory animals (mice, rats, hamsters and dogs) and humans to obtain the corresponding formula dependencies and calibration curves.

Basis: The work is a preamble for a more extensive analysis of data on the age-related radiosensitivity of animals as applied to the extrapolation of the revealed patterns to humans. The presented introductory review of the history of research in this field showed that the main works were carried out in the 1950s - 1960s, and, much less, in the 1970s, and the results, apparently, produced almost nothing for practical radiation medicine and radiation safety. Investigations of the relationship between the age and the radiosensitivity for the human which were exposed to total irradiation in significant doses were practically not found although such data are important because of the permanent threat of nuclear incidents and terrorism. In this regard, the quantitative transfer of the corresponding dependencies, identified for various species of animals, on the situation with acute human radiation syndrome continues to be relevant. In its entirety, according to our analysis of sources it has not been carried out until now, including the documents of UNSCEAR, ICRP, WHO, and others.

Material and methods: Data on physiological age periods and their boundaries for animals and humans, published in reliable scientific sources, were used for calculations and general analysis. Based on the extracted values (from tables and one chart of originals), using the IBM SPSS and Statistica programs, a formula was derived for the ‘standard’ dependencies on ‘age of the animal - age of the human’ and a corresponding calibration schedule was constructed. Both direct and indirect data were used. In the first case (mice, rats, dogs) we used the data for direct comparison of the age periods of animals and humans  and in the second (mice, rats, hamsters) we used the quantitative information about a particular age period for an animal. It allowed us to conduct own comparison of such data with a similar period of human life.

Results: ‘Standard’ formulas were derived and ‘standard’ calibration curves were obtained, which made it possible to compare the age of mice, rats, hamsters and dogs with human age. In parallel, it turned out that many of the so-called ‘calculators’ in the English and Russian-language Internet, which can translate the age of almost any animal into human age (according to the statements of their developers), give the mistakes at comparative estimates with the observed dependencies on the basis of scientific data (difference up to 20-60 %).

Conclusions: The obtained data fill the existing scientific gaps, creating the prerequisites for both comparison of the parameters of the age-related radiosensitivity of laboratory animals and humans (important for radiation safety) and for use in other experimental areas of biomedical disciplines. On the basis of detailed approaches to the problem considered in the paper, it is possible to derive similar relationships for the age of any other animal and human.

Key words: relationship age of animal - age of human, mice, rat, hamster, dog, age-dependent radiosensitivity

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For citation: Koterov AN, Ushenkova LN, Zubenkova ES, Wainson AA, Biryukov AP. The Relationship Between the Age of the Based Laboratory Animals (Mice, Rats, Hamsters and Dogs) and the Age of Human: Actuality for the Age-Ralated Radiosensitivity Problem and the Analysis of Published Data. Medical Radiology and Radiation Safety. 2018;63(1):5-27. DOI: 10.12737/article_5a82e4a3908213.56647014. Russian.

PDF (RUS) Full-text article (in Russian)

Medical Radiology and Radiation Safety. 2015. Vol. 60. No. 1. P. 54-61

RADIATION PHYSICS, TECHNOLOGY AND DOSIMETRY

L.Ya. Klepper

Formation Optimum Doses Fields in Interstitial Radiotherapy as the Problem of the Covering of the Target by Circles of Identical Radius

Central Economic Mathematical Institute of RAS, Moscow, Russia, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. This email address is being protected from spambots. You need JavaScript enabled to view it.

ABSTRACT

The purpose of work consists in description of the problem of optimum sources accommodation in a target at interstitial radiotherapy of malignant tumor by methods of the «Parisian grid» and methods of optimum sources accommodation which are reduced to problems of an optimum covering of a target by identical circles of the minimal radius. Connection between two methods is tracked. It is shown, that particular decisions can be equivalent to the «Parisian grid» method. Examples of the problem decision and the analysis of the decisions received are presented.

Key words: interstitial radiotherapy, optimum distribution of intrastats

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For citation: Klepper LYa. Formation Optimum Doses Fields in Interstitial Radiotherapy as the Problem of the Covering of the Target by Circles of Identical Radius. Medical Radiology and Radiation Safety. 2015;60(1):54-61. Russian.

PDF (RUS) Full-text article (in Russian)

  1. 2015-1- Kovtun-eng
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