JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.
Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.
The journal is included in the List of Russian Reviewed Scientific Journals of the Higher Attestation Commission. Since 2008 the journal has been available on the Internet and indexed in the RISC database which is placed on Web of Science. Since February 2nd, 2018, the journal "Medical Radiology and Radiation Safety" has been indexed in the SCOPUS abstract and citation database.
Brief electronic versions of the Journal have been publicly available since 2005 on the website of the Medical Radiology and Radiation Safety Journal: http://www.medradiol.ru. Since 2011, all issues of the journal as a whole are publicly available, and since 2016 - full-text versions of scientific articles. Since 2005, subscribers can purchase full versions of other articles of any issue only through the National Electronic Library. The editor of the Medical Radiology and Radiation Safety Journal in accordance with the National Electronic Library agreement has been providing the Library with all its production since 2005 until now.
The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.
Since 2017 the journal Medical Radiology and Radiation Safety has switched to digital identification of publications, assigning to each article the identifier of the digital object (DOI), which greatly accelerated the search for the location of the article on the Internet. In future it is planned to publish the English-language version of the journal Medical Radiology and Radiation Safety for its development. In order to obtain information about the publication activity of the journal in March 2015, a counter of readers' references to the materials posted on the site from 2005 to the present which is placed on the journal's website. During 2015 - 2016 years on average there were no more than 100-170 handlings per day. Publication of a number of articles, as well as electronic versions of profile monographs and collections in the public domain, dramatically increased the number of handlings to the journal's website to 500 - 800 per day, and the total number of visits to the site at the end of 2017 was more than 230.000.
The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Issues journals
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 2
DOI: 10.33266/1024-6177-2023-68-2-92-94
B.E. Serebryakov
Recommendations for Amendments to the NRB-99/2009 and to the OSPORB-99/2010
A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: B.E. Serebryakov, e-mail:
This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: In Russia, the main regulatory documents on radiation safety are being adjusted: Radiation Safety Standards (NRB-99/2009) and Basic Sanitary Rules (OSPORB-99/2010). The purpose of the work is to remove and correct certain incorrect provisions of these documents.
Results: The disadvantages of NRB-99/2009 related to the values of the minimum significant activity and the minimum significant specific activity of radionuclides were identified and justified.
In OSPORB-99/2010, the inadmissibility of introducing minimally licensed activity of radionuclides in closed sources of ionizing radiation into Sanitary Rules was identified and justified. An unacceptably large amount of this activity can lead to overexposure of personnel and the public in incidents with these sources.
Conclusions: The following recommendations were made for the adjustment of NRB-99/2009 and OSPORB-99-2010:
– in NRB-99/2009, it is recommended to completely remove the minimally significant specific activities, and use the minimally significant activities in accordance with NRB-76/87.
– in OSPORB-99/2010, it is recommended to remove all provisions related to the minimum licensed activity of radionuclides in closed sources of ionizing radiation.
Key words: radiation safety, regulatory documents, minimally significant activity, minimally significant specific activity, closed sources of ionizing radiation
For citation: Serebryakov BE. Recommendations for Amendments to the NRB-99/2009 and to the OSPORB-99/2010. Medical Radiology and Radiation Safety. 2023;68(2):92–94. (In Russian). DOI: 10.33266/1024-6177-2023-68-2-92-94
References
1. SanPiN 2.6.1.2523 – 09 Radiation Safety Standards (NRB-99/2009) (In Russ.).
2. SP 2.6.1.2612-10 Basic Sanitary Rules for Radiation Safety (OSPORB-99/2010) (In Russ.).
3. Serebryakov B.Ye. About of the Necessary of Corrections of the NRB-99/2009 and OSPORB-99/2010. Meditsinskaya Radiologiya i Radiatsionnaya Bezopasnost = Medical Radiology and Radiation Safety. 2020;65;3:27–30 (In Russ.).
4. International Basic Safety Standards for Protection against Ionizing Radiation and for the Safety of Radiation Sources. Safety Series № 115. IAEA, Vienna, 1997.
5. Serebryakov B.Ye. About of the Necessary the Review of the Government Decision of the Russian Federation from October 19, 2012 No. 1069. Meditsinskaya Radiologiya i Radiatsionnaya Bezopasnost = Medical Radiology and Radiation Safety. 2021;66;2:83–88 (In Russ.).
6. Radiation Safety Standards NRB-76/87 and Basic Sanitary Rules for Working with Radioactive Substances and Other Sources of iONIZING Radiation OSP-72/86. Moscow Publ., 1988 (In Russ.).
7. 10 CFR Part 20 – Standards for Protection Against Radiation.
8. Radiation Protection and Safety of Radiation Sources. General Safety Requirements No. GSR Part 3. Vienna, IAEA, 2015.
9. Dangerous Quantities of Radioactive Material (D-Values) Emergency Preparedness and Response. EPR-D-VALUES 2006. Vienna, IAEA, 2010.
10. 10 CFR Part 835 - Occupational Radiation Protection.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.11.2022. Accepted for publication: 25.01.2023.
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 2
DOI: 10.33266/1024-6177-2023-68-2-95-98
V.N. Klochkov, S.M. Shinkarev, O.A. Kochetkov, V.G. Barchukov, A.V. Simakov
To the Discussion on Amendments to Nrb-99/2009 and to Osporb-99/2010
A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: Vladimir Nikolaevich Klochkov, e-mail:
This email address is being protected from spambots. You need JavaScript enabled to view it.
For citation: Klochkov VN, Shinkarev SM, Kochetkov OA, Barchukov VG, Simakov AV. To the Discussion on Amendments to Nrb-99/2009 and to Osporb-99/2010. Medical Radiology and Radiation Safety. 2023;68(2):95–98. (In Russian). DOI: 10.33266/1024-6177-2023-68-2-95-98
References
1. Vedernikova M.V., Linge I.I., Panchenko S.V., Strizhova S.V., Supatayeva O.A., Utkin S.S. Aktualnyye Voprosy Vneseniya Izmeneniy v Federalnyy Zakon ot 9 Yanvarya 1996 g. № 3-FZ «O Radiatsionnoy Bezopasnosti Naseleniya» = Topical Issues of Amending the Federal Law Dated January 9, 1996 No. 3-FZ “On Radiation Safety of the Population” Moscow Publ., 2020. 22 p. ISBN 978-5-6041296-5-4 (In Russ.).
2. Kochetkov O.A., Klochkov V.N., Panfilov A.P., Usoltsev V.Yu. Scientific and Methodological Support of Radiation Safety in the Organizations of the State Corporation “Rosatom” V.1. Metodicheskoye Obespecheniye Radiatsionnogo Kontrolya v Atomnoy Otrasli = Methodological Support of Radiation Monitoring in the Nuclear Industry. Moscow Publ., 2016. P. 24-40 (In Russ.).
3. Kochetkov O.A., Panfilov A.P., Usoltsev V.Yu., Klochkov V.N., Shinkarev S.M., Tsovyanov A.G., Simakov A.V. Radiation Hygiene and Safety of Nuclear Industry. Gigiyena i Sanitariya = Hygiene and Sanitation. 2017;69;9:868-874 (In Russ.).
4. Abramov Yu.V., Klochkov V.N., Kochetkov O.A., Simakov A.V., Nurlybayev K. Current Requirements to Establishment of the Radiation Monitoring System. Apparatura i Novosti Radiatsionnykh Izmereniy = ANRI. 2019;3:3-10 (In Russ.).
5. Simakov A.V., Abramov Yu.V. Radiation Safety Standards and Basic Health Rules for Radiation Safety: Proposal on the Development of New Versions. Meditsinskaya Radiologiya i Radiatsionnaya Bezopasnost = Medical Radiology and Radiation Safety. 2019;64;5:15-19. DOI: 10.12737/1024-6177-2019-64-5-15-19 (In Russ.).
6. Shinkarev S.M., Kochetkov O.A., Klochkov V.N., Barchukov V.G. To Discussion on Amendments to the Federal Law as of 09.01.1996 No. 3-FZ «About Radiation Safety of the Public». Meditsinskaya Radiologiya i Radiatsionnaya Bezopasnost = Medical Radiology and Radiation Safety. 2020;65;3:77-78. DOI 10.12737/1024-6177-2020-65-3-77-78 (In Russ.).
7. Kochetkov O.A., Klochkov V.N., Samoylov A.S., Shandala N.K. Harmonization of the Russian Federation Legislation with Current International Recommendations. Meditsinskaya Radiologiya i Radiatsionnaya Bezopasnost = Medical Radiology and Radiation Safety. 2021;66;6:111–115. DOI: 10.12737/1024-6177-2021-66-6-111-115 (In Russ.).
8. Kochetkov O.A., Klochkov V.N., Samoylov A.S., Shandala N.K, Barchukov V.G., Shinkarev S.M. General Principles of Legal, Standard and Methodical Regulation of Radiation Safety. Meditsinskaya Radiologiya i Radiatsionnaya Bezopasnost = Medical Radiology and Radiation Safety. 2022;61;1:19–26. DOI: 10.12737/1024-6177-2022-67-1-19-26 (In Russ.).
9. Gubin A.T., Sakovich V.A. On Some Conceptual Issues of Changing the Federal Law «On Radiation Safety of the Population». Meditsinskaya Radiologiya i Radiatsionnaya Bezopasnost = Medical Radiology and Radiation Safety. 2020;65;6:83-84. DOI: 10.12737/1024-6177-2020-65-6-83-84 (In Russ.).
10. Serebryakov B.Ye. About of the Necessary of Corrections of the NRB-99/2009 and OSPORB-99/2010. Meditsinskaya Radiologiya i Radiatsionnaya Bezopasnost = Medical Radiology and Radiation Safety. 2020;65;3:27-30. DOI: 10.12737/1024-6177-2020-65-3-27-30 (In Russ.).
11. Serebryakov B.Ye. On the Need to Revise Government Decree No. 1069 dated 10/19/2012. Meditsinskaya Radiologiya i Radiatsionnaya Bezopasnost = Medical Radiology and Radiation Safety. 2021;66;2:83-88 (In Russ.).
12. International Basic Safety Standards for Protection against Ionizing Radiation and for the Safety of Radiation Sources, Safety Series No. 115. Vienna, IAEA, 1996.
13. Application of the Concepts of Exclusion, Exemption and Clearance: Safety Guide. Vienna, International Atomic Energy Agency, 2004. ISBN 92-0-109404-3.
14. Derivation of Activity Concentration Values for Exclusion, Exemption and Clearance. Vienna, International Atomic Energy Agency, 2005. ISBN 92–0–113104–6.
15. Radiation: Protection and Safety of Radiation Sources: International Basic Safety Standards. Vienna, International Atomic Energy Agency, 2014. ISBN 978–92–0–135310–8.
16. Principles and Methods for Establishing Concentrations and Quantities (Exemption values) below which Reporting is not Required in the European Directive. Commission of the European Communities, 1993, Doc. XI-028/93.
17. Licensing Requirements for Land Disposal of Radioactive Wastes: Code of Federal Regulations, Title 10, Part 61, U.S. Nuclear Regulatory Commission, Government Printing Office, Washington, DC, 1987.
18. IAEA-EPR-D-Values 2006. Dangerous Quantities of Radioactive Material (D-Values). Vienna, IAEA, 2006.
19. URL: https://www.ecfr.gov/current/title-10/chapter-III/part-835?toc=1.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.11.2022. Accepted for publication: 25.01.2023.
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 1
DOI: 10.33266/1024-6177-2023-68-1-5-14
V.A. Nikitina, T.A. Astrelina, V.Yu. Nugis, I.V. Kobzeva, E.E. Lomonosova,
Yu.B. Suchkova, T.F. Malivanova, V.A. Brunchukov, D.Yu. Usupzhanova,
V.A. Brumberg, A.A. Rastorgueva, E.I. Dobrovolskaya, T.V. Karaseva,
M.G. Kozlova, M.V. Pustovalova, A.K. Chigasova, N.Yu. Vorobyeva,
A.N. Osipov, A.S. Samoilov
Cytogenetic Analysis of the Cell Line of Multipotent Human Mesenchymal Stromal Cells during Long-Term Cultivation after Exposure to X-Ray Radiation at Low and Medium Doses
A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: V.A. Nikitina, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To evaluate the frequency and spectrum of chromosome aberrations under X-Ray exposure at doses of 80, 250, and 1000 mGy in a human multipotent mesenchymal stromal cell (MMSC) cell line during long-term cultivation.
Material and methods: MMSCs were isolated from human gingival mucosa by an enzymatic method and cultured in a serum-free medium. The presence of surface antigens was determined using the method of flow cytometry. The ability of the cell line to differentiate in the osteogenic, adipogenic, and chondrogenic directions was studied using induction media. Authentication was performed by genotyping of polymorphic STR loci, cytogenetic analysis was performed by multicolor fluorescent in situ hybridization (mFISH). Irradiation was carried out on an X-ray biological unit RUB RUST-M1 (Russia) at a dose rate of 40 mGy/min, a voltage of 100 kV, and a current of 0.8 mA.
Results: At the first passage after irradiation, a statistically significant increase in the frequency of non-clonal CA compared with the control was recorded at a dose of 80, but not 250 and 1000 mGy. At the late stages of cultivation, the average frequency of breaks per chromosome in the group of non-irradiated cells did not differ from the values obtained after irradiation at doses of 80, 250, and 1000 mGy (p > 0.05). However, in MMSCs irradiated at a dose of 80 mGy, damage occurred more often in pairs of chromosomes 6 and 10, and at a dose of 1000 mGy, in a pair of chromosomes 9. A single irradiation of MMSCs in vitro did not affect the growth and progression of MMSCs characteristic of the studied primary cell line, of clonal cells with chromosome translocations and monosomy X, but led to an increase in the representation of a clone with tetrasomy 8. The total number of random clones with chromosome translocations that arose de novo increased after irradiation at a dose of 1000 mGy.
Conclusion: Minor fluctuations in the proportion of cells with non-clonal CA, depending on the dose received in the early stages after irradiation (passage 1–4), disappeared at the later stages of cultivation (passage 8–14). There were no differences in mean frequencies between irradiated and non-irradiated MMSCs, but after irradiation, damage to some chromosomes could occur more frequently than others. A single X-ray irradiation of MMSCs can promote the growth and progression of primary pathological cytogenetic clones, regardless of the dose received, as well as an increase in the total number of de novo cell clones with chromosomal translocations that have arisen. A single X-ray irradiation of MMSCs can promote the growth and progression of primary pathological cytogenetic clones, regardless of the dose received, as well as an increase in the total number of de novo cell clones with chromosomal translocations that have arisen.
Keywords: mesenchymal multipotent stromal cells, chromosome aberrations, mFISH, X -ray irradiation, low doses
For citation: Nikitina VA, Astrelina TA, Nugis VYu, Kobzeva IV, Lomonosova EE, Suchkova YuB, Malivanova TF, Brunchukov VA, Usupzhanova DYu, Brumberg VA, Rastorgueva AA, Dobrovolskaya EI, Karaseva TV, Kozlova MG, Pustovalova MV, Chigasova AK, Vorobyeva NYu, Osipov AN, Samoilov AS. Cytogenetic Analysis of the Cell Line of Multipotent Human Mesenchymal Stromal Cells during Long-Term Cultivation after Exposure to X-Ray Radiation at Low and Medium Doses. Medical Radiology and Radiation Safety. 2023;68(1):5–14. (In Russian). DOI: 10.33266/1024-6177-2023-68-1-5-14
References
1. Niwa O., Barcellos-Hoff M.H., Globus R.K., Harrison J.D., Hendry J.H., Jacob P., et al. ICRP Publication 131: Stem Cell Biology with Respect to Carcinogenesis Aspects of Radiological Protection. Ann. ICRP. 2015;44;3-4:7-357. DOI: 10.1177/0146645315595585.
2. Hendry J.H., Niwa O., Barcellos-Hoff M.H., Globus R.K., Harrison J.D., Martin M.T., et al. ICRP Publication 131: Stem Cell Biology with Respect to Carcinogenesis Aspects of Radiological Protection. Ann. ICRP. 2016;45;1:239-252. DOI: 10.1177/0146645315621849.
3. Morikawa S., Mabuchi Y., Kubota Y., Nagai Y., Niibe K., Hiratsu E., et al. Prospective Identification, Isolation, and Systemic Transplantation of Multipotent Mesenchymal Stem Cells in Murine Bone Marrow. J. Exp. Med. 2009;206;11:2483-2496. DOI: 10.1084/jem.20091046.
4. Cairns J. Mutation Selection and the Natural History of Cancer. Nature. 1975;255:197–200. DOI: 10.1038/255197a0.
5. Ilin L.A., Rozhdestvenskiy L.M., Koterov A.N., Borisov N.M. Aktualnaya Radiobiologiya = Actual Radiobiology. A Course of Lectures. Moscow Publ., 2015. 240 p. ISBN 978-5-383-00932-1 (In Russ.).
6. Gorbunova V.N., Baranov V.S. Vvedeniye v Molekulyarnuyu Diagnostiku i Genoterapiyu Nasledstvennykh Zabolevaniy = Introduction to Molecular Diagnostics and Gene Therapy of Hereditary Diseases. A Textbook for Students of Medical Universities. St. Petersburg Publ., 1997. 287 p. ISBN 5-87685-076-4 (In Russ.).
7. Gothe H.J., Minneker V., Roukos V. Dynamics of Double-Strand Breaks: Implications for the Formation of Chromosome Translocations. Adv. Exp. Med. Biol. 2018;1044:27-38. DOI:10.1007/978-981-13-0593-1_3.
8. Terskikh V.V., Vasilyev A.V., Vorotelyak Ye.A. Polarization and Asymmetric Division of Stem Cells. Tsitologiya. 2007;49;11:933-938 (In Russ.).
9. Bochkov N.P., Nikitina V.A. Cytogenetics of Human Stem Cells. Molekulyarnaya Meditsina = Molecular Medicine. 2008;3:40-47 (In Russ.).
10. Chen M.F., Lin C.T., Chen W.C., Yang C.T., Chen C.C., Liao S.K., et al. The Sensitivity of Human Mesenchymal Stem Cells to Ionizing Radiation. Int. J. Radiat. Oncol. Biol. Phys. 2006;66;1:244-253. doi:10.1016/j.ijrobp.2006.03.062.
11. Fekete N., Erle A., Amann E.M., Fürst D., Rojewski M., Langonné A., et al. Effect of High-Dose Irradiation on Human Bone-Marrow-Derived Mesenchymal Stromal Cells. Tissue Engineering Part C Methods. 2015;21;2:112-122. DOI: 10.1089/ten.TEC.2013.0766.
12. Nicolay N.H., Lopez Perez R., Saffrich R., Huber P.E. Radio-Resistant Mesenchymal Stem Cells: Mechanisms of Resistance and Potential Implications for the Clinic. Oncotarget. 2015;6;23:19366-19380. DOI: 10.18632/oncotarget.4358.
13. Sugrue T., Lowndes N.F., Ceredig R. Mesenchymal Stromal Cells: Radio-Resistant Members of the Bone Marrow. Immunol Cell. Biol. 2013;91;1:5-11. DOI: 10.1038/icb.2012.61.
14. Rieger K., Marinets O., Fietz T., Körper S., Sommer D., Mücke C., et al. Mesenchymal Stem Cells Remain of Host Origin Even a Long Time after Allogeneic Peripheral Blood Stem Cell or Bone Marrow Transplantation. Exp. Hematol. 2005;33;5:605-611. doi: 10.1016/j.exphem.2005.02.004.
15. Lomonosova Ye.Ye., Nugis V.Yu., Snigireva G.P., Kozlova M.G., Nikitina V.A., Galstyan I.A. Cytogenetic Analysis of Peripheral Blood Lymphocyte Cultures of the Patient in the Long Term after Emergency Irradiation Using the Tricolor FISH Method. Radiatsionnaya Biologiya. Radioekologiya = Radiation Biology. Radioecology. 2022;62;1:5-17. DOI: 10.31857/S0869803122010064 (In Russ.).
16. Dominici M., Le Blanc K., Mueller I., Slaper-Cortenbach I., Marini F., Krause D., et al. Minimal Criteria for Defining Multipotent Mesenchymal Stromal Cells. The International Society for Cellular Therapy Position Statement. Cytotherapy. 2006;8;4:315-317. doi: 10.1080/14653240600855905.
17. Shaffer L.G., McGowan-Jordan J., Schmid M. ISCN 2013: an International System for Human Cytogenetic Nomenclature - 2013. Basel, Karger, 2013.
18. Nikitina V.A., Astrelina T.A., Kobzeva I.V., Nugis V.Yu., Lomonosova Ye.Ye., Semina V.V., et al. The Cytogenetic Characteristic of Mesenchymal Multipotent Stromal Cell Diploid Lines. Tsitologiya. 2021;63;3:207-220. DOI 10.31857/S0041377121030081 (In Russ.).
19. Bochkov N.P., Voronina Ye.S., Katosova L.D., Nikitina V.A. Cytogenetic Study of Multipotent Mesenchymal Stromal Cells of Humans in the Process of Cultivation. Meditsinskaya Genetika = Medical Genetics. 2009;12;90:3-6 (In Russ.).
20. Koltsova A.M., Zenin V.V., Petrosyan M.A., Turilova V.I., Yakovleva T.K., Polyanskaya G.G. Isolation and Characterization of Mesenchymal Stem Cells Derived from Different Regions of the Placenta of the Same Donor. Tsitologiya. 2020;62;9:623-637. DOI:10.31857/S0041377120090035 (In Russ.).
21. Polyanskaya G.G. Comparative Analysis of the Lines of human Mesenchymal Stem Cells Derived in the Collection of Cell Cultures of Vertebrates. (Review). Kletochnyye Kultury. 2018;34:3-18 (In Russ.).
22. Barkholt L., Flory E., Jekerle V., Lucas-Samuel S., Ahnert P., Bisset L., et al. Risk of Tumorigenicity in Mesenchymal Stromal Cell-Based Therapies - Bridging Scientific Observations and Regulatory Viewpoints. Cytotherapy. 2013;15;7:753-759. DOI:10.1016/j.jcyt.2013.03.005.
23. Pustovalova M., Grekhova A., Astrelina Т., Nikitina V., Dobrovolskaya E., Suchkova Y., et al. Accumulation of Spontaneous γH2AX Foci in Long-Term Cultured Mesenchymal Stromal Cells. Aging. 2016;8;12:3498-3506. DOI: 10.18632/aging.101142.
24. Pustovalova M., Astrelina Т.A., Grekhova A., Vorobyeva N., Tsvetkova A., Blokhina T., et al. Residual γH2AX Foci Induced by low Dose X-Ray Radiation in Bone Marrow Mesenchymal Stem Cells Do Not Cause Accelerated Senescence in the Progeny of Irradiated Cells. Aging. 2017;9;11:2397-2410. DOI: 10.18632/aging.101327.
25. Nikitina V., Nugis V., Astrelina T., Zheglo D., Kobzeva I., Kozlova M., et al. Pattern of Chromosomal Aberrations Persisting Over 30 Years in a Chernobyl Nuclear Power Plant Accident Survivor: Study Using mFISH. J. Radiat. Res. 2022;63;2:202-212. DOI:10.1093/jrr/rrab131.
26. Velichko A.K., Razin S.V., Kantidze O.L. Cellular Response to DNA Damage Occurring in Ribosomal Genes. Molekulyarnaya Biologiya = Molecular Biology. 2021;55;2:210-222. DOI: 10.31857/S0026898421020142 (In Russ.).
27. Hemsing A.L., Hovland R., Tsykunova G., Reikvam H. Trisomy 8 in Acute Myeloid Leukemia. Expert Rev. Hematol. 2019;12;11:947-958. DOI: 10.1080/17474086.2019.1657400.
28. Dugan L.C., Bedford J.S. Are Chromosomal Instabilities Induced by Exposure of Cultured Normal Human Cells to Low- or High-LET Radiation? Radiat. Res. 2003;159;3:301-311. DOI:10.1667/0033-7587(2003)159[0301:aciibe]2.0.co;2.
29. Serakinci N., Guldberg P., Burns J.S., Abdallah B., Schrødder H., Jensen T., et al. Adult Human Mesenchymal Stem Cell as a Target for Neoplastic Transformation. Oncogene. 2004;23;29:5095-5098. DOI:10.1038/sj.onc.1207651.
30. Nikitina V., Astrelina T., Nugis V., Ostashkin A., Karaseva T., Dobrovolskaya E., et al. Clonal Chromosomal and Genomic Instability During Human Multipotent Mesenchymal Stromal Cells Long-Term Culture. PLoS One. 2018;13;2:e0192445. DOI:10.1371/journal.pone.0192445.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.09.2022. Accepted for publication: 25.11.2022.
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 2
DOI: 10.33266/1024-6177-2023-68-2-99-104
Leonid Andreevich Ilyin (on the Occasion of His 95th Birthday)
I.L. Efimova
A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia.
Contact person: Efimova Irina Leonidovna, e-mail:
This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Full member of the Russian Academy of Sciences, Doctor of Medical Sciences, Professor Leonid Andreevich Ilyin is a recognized world authority in the field of radiation medicine, biomedical and hygienic aspects of radiation protection. From 1968 to 2008 for 40 years – director and scientific director of the State Scientific Center of the Order of Lenin of the Institute of Biophysics. Since 2008 – Honorary President of the Federal State Budgetary Institution «State Research Center of the Russian Federation – A.I. Burnazyan Federal Medical Biophysical Center» (after the merger of the Institute of Biophysics and Clinical Hospital No. 6).
Thanks to the work of Academician Ilyin, his students and colleagues, highly effective drugs for the prevention and treatment of acute radiation injuries were created, tested and entered into domestic practice. Repeatedly took part as a supervisor in testing the developed drugs in field conditions. He is a veteran of special risk units. L.A. Ilyin is the first scientist in the world who developed and substantiated the forecast of the radiological consequences of the Chernobyl disaster, subsequently confirmed by leading foreign and domestic experts.
Keywords: biophysics, radiation safety, Chernobyl acsident, Ilyin
For citation: Efimova IL. Leonid Andreevich Ilyin (on the occasion of his 100th birthday). Medical Radiology and Radiation Safety. 2023;68(2):99-104. DOI: 10.33266/1024-6177-2022-67-4-99-104
PDF (RUS) Full-text article (in Russian)
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 1
DOI: 10.33266/1024-6177-2023-68-1-15-18
D.V. Saleeva1, L.M. Rozhdestvensky1, N.F. Raeva1, E.S. Vorobeva1,
G.D. Zasukhina1,2
Mechanisms of Antitumor Activity of Low Doses of Radiation Associated with Activation of Cells’ Defense System
1A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2Institute of General Genetics, Moscow, Russia
Contact person: D.V. Saleeva, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Background: The effects of ionizing radiation (IR) involve a highly orchestrated series of events in cells, including DNA damage and repair, cell death, and changes in the level of proliferation associated with the stage of the cell cycle. A large number of existing studies in literature have examined the activity of genes and their regulators in mammalian cells in response to high doses of ionizing radiation. Although there are many studies, the research in effect of low doses of ionizing radiation remains limited. Though much progress has been made in understanding the basic principles of effects of low doses radiation on individual components of biological systems, less is known about how low doses affect target molecules and regulate the cellular networks (e.g., activation of the immune system, genes and their regulators in the phenomenon of hormesis, the formation of an adaptive response). These observations determined the purpose of the work: to investigate the activity of genes and non-coding RNAs (long non-coding RNAs and microRNAs) in various organs of mice with transplanted Lewis carcinoma after low doses radiation.
Material and methods: 24 female mice C57Bl/6 were transplanted subcutaneously with Lewis carcinoma cells (105 cells in 0.2 ml of Hanks’ solution). Total 4-fold X-ray irradiation with an interval of 4 days at a dose of 0.075 Gy (0.85 Gy/min) was performed on the RUST M1 from 6 days after transplantation; the tumor size was measured daily. The mice were divided into the following groups: biocontrol, biocontrol+irradiation, tumor and tumor+irradiation. On the 19th day from the beginning of the experiment, the mice were euthanized. The expression profiles of mRNA genes, long non-coding RNAs and microRNAs controlling the response to radiation were determined in the bone marrow, thymus, spleen and tumor of mice.
Results: Fractionated low doses irradiation of mice with transplanted Lewis carcinoma caused a growth decrease of implanted tumour cells compared to the similar group without irradiation. At the same time, there was an activation of oncosuppressors, and a decrease in the activity of oncogenes in the thymus and spleen of mice with tumor and irradiation. In the tumor group, without irradiation, the number of activated oncogenes prevailed over the number of inactivated ones.
Conclusion: Thus, the low doses radiation exposure led to the activation of antitumor immunity in mice, which emerged in slowing tumor growth in animals and was represented in the induction of oncosuppressors and inhibition of oncogenes expression.
Keywords: low doses of radiation, Lewis carcinoma, non-coding RNA, oncogenes, oncosuppressors, mice
For citation: Saleeva DV, Rozhdestvensky LM, Raeva NF, Vorobeva ES, Zasukhina GD. Mechanisms of Antitumor Activity of Low Doses of Radiation Associated with Activation of Cells’ Defense System. Medical Radiology and Radiation Safety. 2023;68(1):15–18.
(In Russian). DOI: 10.33266/1024-6177-2023-68-1-15-18
References
1. Sharma D.N., Guleria R., Wig N., Mohan A., Rath G., Subramani V., et al. Low-Dose Radiation Therapy for COVID-19 Pneumonia: a Pilot Study. Br. J. Radiol. 2021;94;1126:20210187. DOI: 10.1259/bjr.20210187.
2. Ceyzériat K., Tournier B.B., Millet P., Dipasquale G., Koutsouvelis N., Frisoni G.B., et al. Low-Dose Radiation Therapy Reduces Amyloid Load in Young 3xTg-AD Mice. J. Alzheimers Dis. 2022;86;2:641-653. DOI: 10.3233/JAD-215510.
3. Lumniczky K., Impens N., Armengol G., Candéias S., Georgakilas A.G., Hornhardt S., et al. Low Dose Ionizing Radiation Effects on the Immune System. Environ. Int. 2021;149:106212. DOI: 10.1016/j.envint.2020.106212.
4. Dahl H., Eide D.M., Tengs T., Duale N., Kamstra J.H., Oughton D.H. et al. Perturbed Transcriptional Profiles after Chronic Low Dose Rate Radiation in Mice. PLoS One. 2021;16;8:e0256667. DOI: 10.1371/journal.pone.0256667. eCollection 2021.
5. Михайлов В.Ф., Салеева Д.В., Рождественский Л.М. и др. Активность генов и некодирующих РНК как подход к определению ранних биомаркеров радиоиндуцированного опухолеобразования у мышей // Генетика. 2021. Т. 57, № 10, С. 1131-1140. DOI: 10.31857/S0016675821100076. [Mikhaylov V.F., Saleyeva D.V., Rozhdestvenskiy L.M., et al. Activity of Genes and Non-Coding RNA as an Approach to Early Biomarkers Determination of Radiation-Induced Cancer in Mice. Genetika = Russian Journal of Genetics. 2021;57;10:1131-1140. DOI: 10.31857/S0016675821100076. DOI: 10.31857/S0016675821100076 (In Russ.)].
6. Herrera F.G., Romero P., Coukos G. Lighting up the Tumor Fire with Low-Dose Irradiation. Trends Immunol. 2022;43;3:173-179. DOI: 10.1016/j.it.2022.01.006.
7. Wan X., Fang M., Chen T., Wang H., Zhou Q., Wei Y., et al. The Mechanism of Low-Dose Radiation-Induced Upregulation of Immune Checkpoint Molecule Expression in Lung Cancer Cells. Biochem. Biophys. Res. Commun. 2022;608:102-107. DOI: 10.1016/j.bbrc.2022.03.158.
8. López-Nieva P., González-Vasconcellos I., González-Sánchez L., Cobos-Fernández M.A., Ruiz-García S., Pérez R.S., et al. Differential Molecular Response in Mice and Human Thymocytes Exposed to a Combined-Dose Radiation Regime. Scientific Reports. 2022;12:3144. DOI: https://doi.org/10.1038/s41598-022-07166-8.
9. Zhou L., Zhang X., Li H., Niu C., Yu D., Yang G., et al. Validating the Pivotal Role of the Immune System in Low-Dose Radiation-Induced Tumor Inhibition in Lewis Lung Cancer-Bearing Mice. Cancer Med. 2018;7;4:1338-1348. DOI: 10.1002/cam4.1344.
10. Brown G. Oncogenes, Proto-Oncogenes, and Lineage Restriction of Cancer Stem Cells. Int. J. Mol. Sci. 2021;22;18:9667. DOI: 10.3390/ijms22189667.
11. Qi Z., Guo S., Li C., Wang Q., Li Y., Wang Z. Integrative Analysis for the Roles of lncRNAs in the Immune Responses of Mouse PBMC Exposed to Low-Dose Ionizing Radiation. Dose-Response. 2020;18;1:1559325820913800. DOI:10.1002/cam4.1344.
12. Khan M.G.M., Wang Y. Advances in the Current Understanding of How Low-Dose Radiation Affects the Cell Cycle. Cells. 2022;11;3:356. DOI:10.3390/cells11030356.
13. Rusin M., Ghobrial N., Takacs E., Willey J.S., Dean D. Changes in Ionizing Radiation Dose Rate Affect Cell Cycle Progression in Adipose Derived Stem Cells. PLoS One. 2021;16;4:e0250160. DOI: 10.1371/journal.pone.0250160.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was carried out on the topic of the A.I. Burnazyan State Scientific Research Center «Technology-2» (state task No. 10.009.20.800) and on the topic of the N.I. Vavilov Institute of General Genetics of the Russian Academy of Sciences (state task No. 0112-2019-0002).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.09.2022. Accepted for publication: 25.11.2022.