JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.
Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.
The journal is included in the List of Russian Reviewed Scientific Journals of the Higher Attestation Commission. Since 2008 the journal has been available on the Internet and indexed in the RISC database which is placed on Web of Science. Since February 2nd, 2018, the journal "Medical Radiology and Radiation Safety" has been indexed in the SCOPUS abstract and citation database.
Brief electronic versions of the Journal have been publicly available since 2005 on the website of the Medical Radiology and Radiation Safety Journal: http://www.medradiol.ru. Since 2011, all issues of the journal as a whole are publicly available, and since 2016 - full-text versions of scientific articles. Since 2005, subscribers can purchase full versions of other articles of any issue only through the National Electronic Library. The editor of the Medical Radiology and Radiation Safety Journal in accordance with the National Electronic Library agreement has been providing the Library with all its production since 2005 until now.
The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.
Since 2017 the journal Medical Radiology and Radiation Safety has switched to digital identification of publications, assigning to each article the identifier of the digital object (DOI), which greatly accelerated the search for the location of the article on the Internet. In future it is planned to publish the English-language version of the journal Medical Radiology and Radiation Safety for its development. In order to obtain information about the publication activity of the journal in March 2015, a counter of readers' references to the materials posted on the site from 2005 to the present which is placed on the journal's website. During 2015 - 2016 years on average there were no more than 100-170 handlings per day. Publication of a number of articles, as well as electronic versions of profile monographs and collections in the public domain, dramatically increased the number of handlings to the journal's website to 500 - 800 per day, and the total number of visits to the site at the end of 2017 was more than 230.000.
The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Issues journals
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 4
DOI: 10.33266/1024-6177-2023-68-4-75-80
V.I. Kobylyansky1, T.V. Kudasheva2, M.G. Berezina2, T.M. Magomedov3
Studying the Aerodynamic Characteristics of the Macrotech
and Evaluation of the Possibilities of Its Use for Dynamic Aerosol Scintigraphy
1 Research Institute of Pulmonology, Moscow, Russia
2 Federal Scientific and Clinical Center, Moscow, Russia
3 Russian Research Institute of Physical, Technical and Radio Engineering Measurements, Zelenograd, Moscow region, Russia
Contact person: V.I. Kobylyansky, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Introduction: The leading protective mechanism of the lungs is the processes of deposition of inhaled substances and mucociliary clearance (MCC), the optimal method for studying which is dynamic radioaerosol scintigraphy. are not available on the market. The applicant in this regard for a number of characteristics is the radiopharmaceutical from albumin, produced in the Russian Federation under the brand name Macrotech (M). It is used for perfusion scintigraphy to verify primarily pulmonary embolism and its ability to study deposition of inhalants and MCC has not been studied.
Purpose: To study the aerodynamic properties of M dispersion and to determine the possibilities of its use for dynamic radioaerosol scintigraphy of the lungs in order to assess the processes of deposition of inhaled substances and MCC.
Material and methods: To study the aerodynamic properties of M, on which the assessment of the deposition of inhaled substances and MCC significantly depends, we studied the dispersion of its particles in different states, and studied them in shape and morphology. An ultrasonic inhaler TuR USI-50 (Germany) generated an aerosol from a suspension of M in distilled water. To study the dispersion in air, laser spectrometry was used using the Spraytec Malvern Instruments system (Great Britain). The protein content in the initial suspension and dispersible aerosol, collected in the form of a condensate, was determined using an Immulite 2000 XPi immunochemical analyzer (Siemens, USA).The shape and morphology of the particles were studied using scanning electron microscopy using.
Results: The study of the aerodynamic properties of the dispersion of M indicated that its particles are involved in the dynamics of the movement of the airflow and the flight of water particles generated by the inhaler. The dispersity of the aerosol generated from the suspension M averaged about 5 μm and did not significantly depend on the concentration of the radiopharmaceutical and did not depend on the studied dispersion intensity and airflow rate set using an inhaler. The morphology of M particles was characterized by a complex shape and roughness.
Conclusion: The aerodynamic characteristics of M are not optimal for studying the processes of deposition and MCC. However, a final verdict requires a direct assessment of the deposition of the inhaled radioaerosol generated from this preparation.
Keywords: dynamic aerosol scintigraphy, albumin macroaggregates, macrotech, dispersion aerosol, shape and morphology of particles, inhalant deposition, mucociliary clearance
For citation: Kobylyansky VI, Kudasheva TV, Berezina MG, Magomedov TM. Studying the Aerodynamic Characteristics of the Macrotech and Evaluation of the Possibilities of Its Use for Dynamic Aerosol Scintigraphy. Medical Radiology and Radiation Safety. 2023;68(4):75–80. (In Russian). DOI:10.33266/1024-6177-2023-68-4-75-80
References
1. Krivonogov N.G., Zavadovskiy K.V. Radionuclide Diagnostics in Pulmonology. Natsionalnoye Rukovodstvo po Radionuklidnoy Diagnostike = National Guide on Radionuclide Diagnostics. V.2 v. Ed. Lishmanov Yu.B., Chernov V.I. Tomsk Publ., 2010. P. 163-190 (In Russ.).
2. Chokkappan K., Kannivelu A., Srinivasan S., Babut S.B. Review of Diagnostic Uses of Shunt Fraction Quantification with Technetium-99m Macroaggregated Albumin Perfusion Scan as Illustrated by a Case of Osler-Weber-Rendu Syndrome. Ann. Thorac. Med. 2016;11;2:155-160. doi: 10.4103/1817-1737.180020.
3. Kobylyanskiy V.I. Mukotsiliarnaya Sistema. Fundamentalnyyei Prikladnyye Aspekty = Mucociliary System. Fundamental and Applied Aspects. Moscow Binom Publ., 2008. 416 p. (In Russ.).
4. Kobylyanskiy V.I. Methods for Studying the Mucociliary System: Possibilities and Prospects. Terapevticheskiy Arkhiv = Therapeutic Archive. 2001;73;3:73-76 (In Russ.).
5. Grosser O.S., Ruf J., Kupitzm D., Pethe A., Ulrich G., Genseke P., et al. Pharmacokinetics of 99mTc-MAA- and 99mTc-HAS Microspheres Used in Pre-Radioembolization Dosimetry: Influence on the Liver–Lung Shunt. Journal of Nuclear Medicine. 2016;57;6:925-927. doi:10.2967/jnumed.115.169987.
6. Scheuch G., Bennett W., Borgström L., Clark A., Dalby R., Dolovich M., et al. Deposition, Imaging, and Clearance: What Remains to be Done? Journal of Aerosol Medicine and Pulmonary Drug. Delivery. 2010;23;Suppl 2:S39-S57. http://doi.org/10.1089/jamp.2010.0839.
7. Ball D.R., McGuiro B.E., Chapter 6. Benumof and Hagbergs Airway Management. 2013. P. 159-183. https://doi.org/10.1016/B978-1-4377-2764-7.00006-3.
8. Canziani L., Marenco M., Cavenaghi G., Manfrinato G., Taglietti A., Girella A., et al. Chemical and Physical Characterization of Macroaggregated Human Serum Albumin: Strength and Specificity of Bonds with 99mTc and 68Ga. Molecules. 2022;27:404. doi.org/ 10.3390/molecules27020404.
9. Ament S., Buchholz H.G., Bausbacher N., Brochhausen C., Graf F., Miederer M., et al. PET Lung Ventilation/Perfusion Imaging Using 68Ga Aerosol (Galligas) and 68Ga-Labeled Macroaggregated Albumin. V.194. Theranostics, Gallium-68, and Other Radionuclides. Recent Results in Cancer Research. Ed. Baum R., Rösch F. Springer, Berlin, Heidelberg, 2013. https://doi.org/10.1007/978-3-642-27994-2_22.
10. Gandhi S.J., Babu S., Subramanyam P., Shanmuga Sundaram P. Tc-99m Macro Aggregated Albumin Scintigraphy - Indications Other than Pulmonary Embolism: A Pictorial Essay. Indian J. Nucl. Med. 2013;28;3:152-62. doi: 10.4103/0972-3919.119546.
11. Fazio F. Clinical Radioaerosol Imaging. Bull. Eur. Phisiol. Resp. 1980;16:134-136.
12. Chambarlain M.J. Factor Influencing оn the Regionдk Deposition of Particles in Man. Clin. Sci. 1983;64:641-547.
13. Morgan W.K.C., Ahmad D., Chambarlain M.Y. The Effect of Exercise on an Inhaled Aerosol. Respir. Physiol. 1984;56:327-338.
14. Kobylyanskiy V.I., Artyushkin A.V. Sposob Opredeleniya Ekskretornoy Funktsii Legkikh = A Method for Determining the Excretory Function of the Lungs. A.s. No. 138982. 1986 (In Russ.).
15. Kobylyanskiy V.I. Sposob Opredeleniya Funktsii Mukotsiliarnogo Apparata Legkikh = A Method for Determining the Function of the Mucociliary Apparatus of the Lungs. A.s. No. 1602469. 1988 (In Russ.).
16. Persico M.G., Marenco M., De Matteis G., Manfrinato G., Cavenaghi G., Sgarella A., et al. 99mTc-68Ga-ICG-Labelled Macroaggregates and Nanocolloids of Human Serum Albumin: Synthesis Procedures of a Trimodal Imaging Agent Using Commercial Kits. Contrast Media Mol. Imaging. 2020;22;2020:3629705. doi: 10.1155/2020/3629705.
17. Hung J.C., Redfern M.G., Mahoney D.W., Thorson L.M., Wiseman G.A. Evaluation of Macroaggregated Albumin Particle Sizes for Use in Pulmonary Shunt Patient Studies. J. Am. Pharm. Assoc. (Wash). 2000;40;1:46-51. doi: 10.1016/s1086-5802(16)31035-x.
18. Pempuev V.M., Cmιnchcnhoɞ V.N., TSɔic G. Xaιupoɞ. Physical and Some Radiochemical Properties of Albumin Microspheres Used in Radionuclide Diagnostics. Isotopenpraxis Isotopes in Environmental and Health Studies. 1979;15;1:22-25. DOI: 10.1080/10256017908544275 (In Russ.).
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.02.2022. Accepted for publication: 27.03.2023.
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 4
DOI: 10.33266/1024-6177-2023-68-4-81-84
A.V. Muravleva, V.E. Goldberg, E.A. Dudnikova, T.L. Kravchuk, R.V. Zelchan,
A.A. Medvedeva, O.D. Bragina, E.I. Simolina, N.O. Popova, V.V. Vysockaja,
V.A. Shatalova, A.N. Rybina, A.V. Goldberg, S.A. Tabakaev, V.I. Chernov
Metabolic 99mTc-1-Thio-D-Glucose SPECT/CT in the Diagnosis
of Brain Metastasis of Genital Diffuse-B-Large Cell Lymphoma (Clinical Case)
Cancer Research Institute, Tomsk National Research Medical Center, Tomsk, Russia
Contact person: A.V. Muravleva, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To demonstrate a rare clinical case of early recurrence of verified primary genital lymphoma and the possibility of 99mTc-1-Thio-D-glucose (99mTc-TG) SPECT/CT using to brain metastasis diagnosis.
Material and methods: A patient with a diagnosis of primary diffuse large B-cell genital lymphoma underwent magnetic resonance imaging (MRI) to assess the extent of the disease. Post-treatment follow-up included 18F-FDG PET/CT. To diagnose brain metastasis, 99mTc-TG SPECT/CT and MRI were performed.
Results: A rare case of early recurrence of diffuse large B-cell lymphoma with a primary local lesion in the genital organs is described. The possibility of modern methods of nuclear medicine in the diagnosis of early recurrence of malignant lymphoma has been demonstrated. 99mTc-TG SPECT/CT and MRI, were useful for visualization of a high metabolic brain tumor at the outpatient stage and recommendation of high-dose therapy according to the scheme MT-R was done.
Conclusion: The article presents a rare clinical case of early recurrence of diffuse large B-cell genital lymphoma. Possibilities of SPECT/CT with 99mTc-TG for visualization of lymphoma metastasis to the brain were demonstrated.
Keywords: diffuse large B-cell genital lymphoma, brain metastasis, single photon emission computed tomography, 99mTc-1-Thio-D-glucose
For citation: Muravleva AV, Goldberg VE, Dudnikova EA, Kravchuk TL, Zelchan RV, Medvedeva AA, Bragina OD, Simolina EI, Popova NO, Vysockaja VV, Shatalova VA, Rybina AN, Goldberg AV, Tabakaev SA, Chernov VI. Metabolic 99mTc-1-Thio-D-Glucose SPECT/CT in the Diagnosis of Brain Metastasis of Genital Diffuse-B-Large Cell Lymphoma (Clinical Case). Medical Radiology and Radiation Safety. 2023;68(4):81–84. (In Russian). DOI:10.33266/1024-6177-2023-68-4-81-84
References
1. Chernov V.I., Dudnikova E.A., Goldberg V.E., Kravchuk T.L., Danilova A.V., Zelchan R.V., Medvedeva A.A., Sinilkin I.G., Bragina O.D., Popova N.O., Goldberg A.V. Positron Emission Tomography in the Diagnosis and Monitoring of Lymphomas. Meditsinskaya Radiologiya i Radiatsionnaya Bezopasnost = Medical Radiology and Radiation Safety. 2018;63;6:41-50 (In Russ.).
2. Rossiyskiye Klinicheskiye Rekomendatsii po Diagnostike i Lecheniyu Zlokachestvennykh Limfoproliferativnykh Zabolevaniy = Russian Clinical Guidelines for the Diagnosis and Treatment OF Malignant Lymphoproliferative Diseases. Ed. Poddubnaya I.V., Savchenko V.G. 2018. 470 p. (In Russ.).
3. Barrington S.F., Mikhaeel N.G., Kostakoglu L., et al. Role of Imaging in the Staging and Response Assessment of Lymphoma: Consensus of the International Conference on Malignant Lymphomas Imaging Working Group. J. Clin. Oncol. 2014;32:3048–3058. DOI: 10.1200/JCO.2013.53.5229.
4. Cheson B.D., Fisher R.I., Barrington S.F., et al. Recommendations for Initial Evaluation, Staging, and Response Assessment of Hodgkin and non-Hodgkin Lymphoma: the Lugano Classification // J. Clin. Oncol. 2014. No. 32. P. 3059–3068. DOI: 10.1200/JCO.2013.54.8800.
5. Dreyling M., Thieblemont C., Gallamini A., et al. ESMO Consensus Conferences: Guidelines on Malignant Lymphoma. Part 2: Marginal Zone Lymphoma, Mantle Cell Lymphoma, Peripheral T-Cell Lymphoma. Ann. Oncol. 2013;24:857–877. DOI:- 10.1093/annonc/mds643.
6. Boellaard R., Delgado-Bolton R., Oyen W.J.G., et al. FDG PET/CT: EANM Procedure Guidelines for Tumour Imaging: Version 2.0. Eur. J. Nucl. Med. Mol. Imaging. 2015;42:328–354. https://doi.org/10.1007/s00259-014-2961-x.
7. Zeltchan R., Medvedeva A., Sinilkin I., et al. Experimental Study of Radiopharmaceuticals Based on Technetium-99m Labeled Derivative of Glucose for Tumor Diagnosis. IOP Conference Series. Materials Science and Engineering. 2016. 012054. DOI. 10.1088/1757-899X/135/1/012054.
8. Zelchan R.V., Medvedeva A.A., Sinilkin I.G. Study of the Functional Suitability of the Tumorotropic Radiopharmaceutical 99mTc-1-thio-D-Glucose in the Experiment. Molekulyarnaya Meditsina = Molecular Medicine. 2018;16;2:54–57. DOI: https://doi.org/10.29296/24999490-2018-03-11 (In Russ.).
9. Chernov V.I., Dudnikova E.A., Medvedeva A.A., Sinilkin I.G. Development Radiopharmaceuticals for Nuclear Medicine in Oncology. Meditsinskaya Vizualizatsiya = Medical Visualization. 2016;2:63–66 (In Russ.).
10. Chernov V.I., Dudnikova E.A., Zelchan R.V., et al. The First Experience of Using 99mTc-1-thio-d-Glucose for Single-Photon Emission Computed Tomography Imaging of Lymphomas. Sibirskiy Onkologicheskiy Zhurnal = Siberian Journal of Oncology. 2018;17;4:81–87. DOI: 10.21294/1814-4861-2018-17-4-81-87 (In Russ.).
11. Dudnikova E.A., Chernov V.I., Muravleva A.V., et al. Metabolic Single-Photon Emission Computed Tomography with the New Radiopharmaceutical 99mTc-1-Thio-D-Glucose in the Diagnosis and Monitoring of the Primary Breast Lymphoma (Case Report). Sibirskiy Onkologicheskiy Zhurnal = Siberian Journal of Oncology. 2020;19;5:145–153. https://doi.org/10.21294/1814-4861-2018-17-4-81-87 (In Russ.).
12. Muravleva A.V., Chernov V.I., Dudnikova E.A., et al. Metabolic Single-Photon Emission Computed Tomography with “99mtc-1-Thio-D-Glucose” ‒ New Possibilities for Hodgkin’s Lymphoma Staging. Rossiyskiy Elektronnyy Zhurnal Luchevoy Diagnostiki = Russian Electronic Journal of Radiology. 2021;11;3:171–177. DOI: 10.21569/2222-7415-2021-11-3-171-177 (In Russ.).
13. Chernov V., Dudnikova E., Zelchan R., Medvedeva A., Rybina A., Bragina O., Goldberg V., Muravleva A., Sörensen J., Tolmachev V. Phase I Clinical Trial Using [99mTc]Tc-1-thio-D-glucose for Diagnosis of Lymphoma Patients. Pharmaceutics. 2022;14:1274.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.02.2022. Accepted for publication: 27.03.2023.
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 3
DOI: 10.33266/1024-6177-2023-68-3-11-15
A.V. Simakov, Y.V. Abramov, N.L. Proskuryakova, T.M. Alferova
Health Physics Criteria for Assessing the Radiation Situation
with Changing Technology at a Nuclear Fuel Cycle Enterprise
A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: Y.V. Abramov, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To select and justify health physics criteria necessary and sufficient in assessing the potential hazard for the personnel and public from the implementation of the planned activity, which can lead to deterioration in the radiation situation at the nuclear fuel cycle (NFC) enterprise.
Results: To promptly assess the feasibility of carrying out an event planned at the NFC enterprise, a methodology has been developed for assessing radiation safety and protection in case of potential deterioration in the radiation situation.
Conclusion: Each planned event that may lead to deterioration in the radiation situation at a NFC enterprise should result in neither significant changes in the radiation situation nor exceeding the established health physics criteria:
• non-exceeding main dose constraints;
• non-increasing the category of potential radiation hazard of the NFC enterprise;
• non-increasing the class of work with unsealed radiation sources;
• permissible increase in the class of working conditions of the personnel based on the findings of special assessment of working conditions.
In a comparative assessment of the competitiveness of radiation technologies, potential change in the radiation situation and the cost of implementing compensatory measures should be pre-evaluated to protect the personnel and public in case of deterioration in the radiation situation following the introduction of new technologies.
Keywords: radiation safety, radiation protection, changing technology, health physics criterion, personnel, measurement uncertainty
For citation: Simakov AV, Abramov YV, Proskuryakova NL, Alferova TM. Health Physics Criteria for Assessing the Radiation Situation with Changing Technology at a Nuclear Fuel Cycle Enterprise. Medical Radiology and Radiation Safety. 2023;68(3):11–15.
(In Russian). DOI: 10.33266/1024-6177-2023-68-3-11-15
References
1. Simakov A.V., Abramov Yu.V., Petrov S.V., Rogozhkin V.Yu., et al. Prognostic Assessment of Changing Radiation Situation when Manufacturing Fuel for PWR Reactor VVER-440 from Regenerated Uranium. Sbornik Tezisov VII Mezhdunarodnogo Simpoziuma Ural Atomnyy = VII International Symposium Ural Atomic. Collection of Abstracts. Ekaterinburg Publ., 1999. P. 5-7 (In Russ.).
2. Simakov A.V., Abramov Yu.V., Petrov S.V., Stepanov S.V., Isayev O.V. Methodic Approaches to Assessing the Contribution of Impurity Radionuclides to the Formation of Effective Doses of Occupational Exposure at NFC Enterprises. Sbornik Tezisov VII Mezhdunarodnogo Simpoziuma Ural Atomnyy = VII International Symposium Ural Atomic. Collection of Abstracts. Ekaterinburg Publ., 1999. P. 3-4 (In Russ.).
3. SanPiN 2.6.1.2523—09. Radiation Safety Standards (NRB-99/2009) (In Russ.).
4. SP 2.6.1.2612-10. Main Medical Rules for Radiation Safety (OSPORB-99/2010) (In Russ.).
5. MU 2.6.1.15 – 06. Decision Making Criteria in the Event of Planned Change in Technology at the NFC Enterprise, the Facility Reconstruction and Change of Functions. Guidelines. Ed. Simakov A.V., Abramov Yu.V., et al. (In Russ.).
6. MU 2.6.1.044 -08. Establishing a Class of Works when Managing Unsealed Radiation Sources. Guidelines. Ed. Simakov A.V., Abramov Yu.V., et al. (In Russ.).
7. Method for Conducting the Special Assessment of Work Conditions (Approved by Order of Ministry of Labor of the Russian Federation Dated November14, 2016, No. 642n); (In Russ.).
8. R 2.6.5.07 - 19. Health Physics Criteria for the Special Assessment and Classification of Work Conditions when Managing Radiation Sources. Guidance. Ed. Simakov A.V., Abramov Yu.V., Proskuryakova N.L., et al. (In Russ.).
9. MU 2.6.5. 08 – 2019. Establishing Categories of Potential Hazard of Radiation Facilities. Guidelines. Ed. Simakov A.V., Abramov Yu.V., Barkovskiy A.N., et al. (In Russ.).
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.01.2022. Accepted for publication: 25.02.2023.
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 3
DOI: 10.33266/1024-6177-2023-68-3-5-10
N.Yu. Vorobyeva1, 2, A.A. Osipov2, A.K. Chigasova3, M.V. Pustovalova1, 4,
D.I. Kabanov1, V.G. Barchukov1, O.A. Kochetkov1, A.N. Osipov1, 2
Comparative Study of Changes in the γh2ax and 53bp1 Foci Number in Human Mesenchymal Stromale Cells Incubated with 3H-thymidine or Tritiated Water
1 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2 N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences Moscow, Russia
3 Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow, Russia
4 Moscow Institute of Physics and Technology, Dolgoprudny, Moscow region, Russia
Contact person: N.Yu. Vorobyeva, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: Comparative study of changes in the number of foci of DNA (DSB) marker proteins (γH2AX and 53BP1) in human mesenchymal stromal cells (MSCs) incubated with 3H-thymidine or HTO for 24, 48, and 72 h.
Material and methods: We used the primary culture of human MSCs of passage 5–6, obtained from the collection of LLC “BioloT” (Russia). A sterile solution of 3H-thymidine or HTO with a specific activity of 100 to 400 MBq/l was added to the nutrient medium and incubated under standard conditions of a CO2 incubator for 24, 48, and 72 hours. To quantify γH2AX foci and the proportion of proliferating cells using antibodies to γH2AX, 53BP1 and Ki67 (a marker protein for cell proliferation), were used, respectively. Statistical analysis of the obtained data was carried out using the statistical software package Statistica 8.0 (StatSoft). To assess the significance of differences between samples, Student’s t-test was used.
Results: Incubation of MSCs with 3H-thymidine with a specific radioactivity of 100-400 MBq/l in the first 24 hours leads to a dose-dependent increase in the number of γH2AX and 53BP1 foci. With a further increase in the incubation time to 48 h and 72 h, a saturation effect is observed ‒ the number of foci reaches a plateau. A statistically significant increase in the number of γH2AX and 53BP1 foci in MSCs incubated with HTO was observed only in actively proliferating cells during the first 24 h of incubation in a medium with specific radioactivity of 300 and 400 MBq/l, after which, with a decrease in proliferative activity, it decreased to control values. Calculations made on the basis of the results of a quantitative analysis of γH2AX and 53BP1 foci after 24 h of incubation of MSCs with tritium compounds obtained in the course of the work show, that under the influence of 3H-thymidine ~ 6 times more DNA double-strand breaks are induced than under the influence of HTO.
Keywords: mesenchymal stromal cells, γH2AX, 53BP1, DNA double-strand breaks, cell proliferation, tritium, incubation
For citation: Vorobyeva NYu, Osipov AA, Chigasova AK, Pustovalova MV, Kabanov DI, Barchukov V., Kochetkov OA, Osipov AN. Comparative Study of Changes in the γh2ax and 53bp1 Foci Number in Human Mesenchymal Stromale Cells Incubated with 3h-thymidine or Tritiated Water. Medical Radiology and Radiation Safety. 2023;68(3):5–10. (In Russian). DOI: 10.33266/1024-6177-2023-68-3-5-10
References
1. Гурьев Д.В., Кочетков О.А., Барчуков В.Г., Осипов А.Н. Биологические эффекты органических и неорганических соединений трития // Медицинская радиология и радиационная безопасность. 2020. Т.65, № 2. С. 5-10. https://doi.org/10.12737/1024-6177-2020-65-2-5-10. [Guryev D.V., Kochetkov O.A., Barchukov V.G., Osipov A.N. Biological Effects of Organic and Inorganic Compounds of the Tritium. Meditsinskaya Radiologiya i Radiatsionnaya Bezopasnost = Medical Radiology and Radiation Safety. 2020;65;2:5-10. https://doi.org/10.12737/1024-6177-2020-65-2-5-10 (In Russ.)].
2. Little M.P., Lambert B.E. Systematic Review of Experimental Studies on the Relative Biological Effectiveness of Tritium. Radiat Environ Biophys. 2008;47;1:71-93. doi: 10.1007/s00411-007-0143-y.
3. Kim S.B., Baglan N., Davis P.A. Current Understanding of Organically Bound Tritium (OBT) in the Environment. Journal of Environmental Radioactivity. 2013;126:83-91. doi: 10.1016/j.jenvrad.2013.07.011.
4. Harrison J.D., Khursheed A., Lambert B.E. Uncertainties in Dose Coefficients for Intakes of Tritiated Water and Organically Bound Forms of Tritium by Members of the Public. Radiation Protection Dosimetry. 2002;98;3:299-311.
5. Alloni D., Cutaia C., Mariotti L., Friedland W., Ottolenghi A. Modeling Dose Deposition and DNA Damage Due to Low-Energy Beta(-) Emitters. Radiation Research. 2014;182;3:322-330. doi: 10.1667/RR13664.1.
6. Rodneva S.M., Osipov A.A., Guryev D.V., Tsishnatti A.A., Fedotov Y.А., Yashkina E.I., et al. Comparative Study of the γH2AX Foci Forming in Human Lung Fibroblasts Incubated in Media Containing Tritium-Labeled Thymidine or Amino Acids. Bulletin of Experimental Biology and Medicine. 2021;172;2:245-9. doi: 10.1007/s10517-021-05370-6.
7. Mladenova V., Mladenov E., Stuschke M., Iliakis G. DNA Damage Clustering after Ionizing Radiation and Consequences in the Processing of Chromatin Breaks. Molecules. 2022;27;5. doi: 10.3390/molecules27051540.
8. Jiang Y. Contribution of Microhomology to Genome Instability: Connection between DNA Repair and Replication Stress. International Journal of Molecular Sciences. 2022;23;21. doi: 10.3390/ijms232112937.
9. Sishc B.J., Davis A.J. The Role of the Core Non-Homologous End Joining Factors in Carcinogenesis and Cancer. Cancers (Basel). 2017;9;7. doi: 10.3390/cancers9070081.
10. Rothkamm K., Barnard S., Moquet J., Ellender M., Rana Z., Burdak-Rothkamm S. DNA Damage Foci: Meaning and Significance. Environ Mol. Mutagen. 2015;56;6:491-504. doi: 10.1002/em.21944.
11. Bushmanov A., Vorobyeva N., Molodtsova D., Osipov A.N. Utilization of DNA Double-Strand Breaks for Biodosimetry of Ionizing Radiation Exposure. Environmental Advances. 2022;8:100207. doi: 10.1016/j.envadv.2022.100207.
12. Scully R., Xie A. Double Strand Break Repair Functions of Histone H2AX. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 2013;750;1-2:5-14. doi: 10.1016/j.mrfmmm.2013.07.007.
13. Shibata A., Jeggo P.A. Roles for 53BP1 in the Repair of Radiation-Induced DNA Double Strand Breaks. DNA Repair. 2020;93:102915. doi: 10.1016/j.dnarep.2020.102915.
14. Vorob’eva N.Y., Kochetkov O.A., Pustovalova M.V., Grekhova A.K., Blokhina T.M., Yashkina E.I., et al. Comparative Analysis of the Formation of γH2AX Foci in Human Mesenchymal Stem Cells Exposed to 3H-Thymidine, Tritium Oxide, and X-Rays Irradiation. Bulletin of Experimental Biology and Medicine. 2018;166;1:178-181. doi: 10.1007/s10517-018-4309-1.
15. Bártová E., Legartová S., Dundr M., Suchánková J. A Role of the 53BP1 Protein in Genome Protection: Structural and Functional Characteristics of 53BP1-Dependent DNA Repair. Aging. 2019;11;8:2488-2511. doi: 10.18632/aging.101917.
16. Panier S, Boulton SJ. Double-strand break repair: 53BP1 comes into focus. Nat Rev Mol Cell Biol. 2014;15(1):7-18. doi: 10.1038/nrm3719.
17. Markova E., Vasilyev S., Belyaev I. 53BP1 Foci as a Marker of Tumor Cell Radiosensitivity. Neoplasma. 2015;62;5:770-776. doi: 10.4149/neo_2015_092.
18. Niotis A., Tsiambas E., Fotiades P.P., Ragos V., Polymeneas G. ki-67 and Topoisomerase IIa Proliferation Markers in Colon Adenocarcinoma. J. BUON. 2018;23;7:24-27.
19. Mennan C., Garcia J., Roberts S., Hulme C., Wright K. A Comprehensive Characterisation of Large-Scale Expanded Human Bone Marrow and Umbilical Cord Mesenchymal Stem Cells. Stem Cell Res Ther. 2019;10;1:99. doi: 10.1186/s13287-019-1202-4.
20. Guo Z., Yang J., Liu X., Li X., Hou C., Tang P.H., et al. Biological Features of Mesenchymal Stem Cells from Human Bone Marrow. Chin. Med. J. (Engl). 2001;114;9:950-953.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was carried out with the support of the RGNF (project No. 22-2400490).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.01.2022. Accepted for publication: 25.02.2023.
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 3
DOI: 10.33266/1024-6177-2023-68-3-16-20
F.S. Torubarov, Z.F. Zvereva, I.A. Galstyan, N.A. Metlyaeva
Features of Clinical Manifestations of the Primary Reaction
in Combined Radiation Injury (Radiation Exposure and Mechanical Head Injury)
A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: Z.F. Zvereva, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To describe, on the basis of literature data, the features of the primary reaction of a person with a combined radiation injury – radiation exposure and mechanical head injury – in order to substantiate the possibility of using the symptoms of the primary reaction to predict the severity of acute radiation sickness (ARS).
Material and methods: Analysis of literature data.
Results: Based on the literature data, the clinical symptoms of the primary reaction in human radiation damage in a different dose range, and traumatic brain injuries are described: – concussion of the brain; – mild brain injury; – moderate brain injury.
To characterize the clinical picture of the combined effects of radiation injury and mechanical head injury, the symptoms of the primary reaction were compared with the symptoms of traumatic brain injury. Such symptoms of the primary reaction as vomiting, nausea, headache, dizziness in the most severe form of traumatic brain injury – a moderate brain injury – are diagnosed somewhat more often than in acute radiation sickness. In less severe forms of traumatic brain injury – concussion, mild brain injury – the frequency and severity of these symptoms are close to ARS.
In the complex clinical picture of combined radiation exposure and traumatic brain injury, the most different symptoms of the two components of combined radiation damage are the state of consciousness and the characteristics of the skin.
Conclusion: Clinical symptoms of the primary reaction to radiation exposure in conditions of combined radiation damage in the presence of head trauma lose their diagnostic significance for the early prognosis of the severity of developing ARS. In the clinical picture of combined radiation exposure and mechanical head injury, the most pronounced leading components should be the state of consciousness and the characteristics of the skin.
Keywords: combined radiation injury, mechanical head injury, primary reaction
For citation: Torubarov FS, Zvereva ZF, Galstyan IA, Metlyaeva NA. Features of Clinical Manifestations of the Primary Reaction in Combined Radiation Injury (Radiation Exposure and Mechanical Head Injury). Medical Radiology and Radiation Safety. 2023;68(3):16–20. (In Russian). DOI: 10.33266/1024-6177-2023-68-3-16-20
References
1. Kombinirovannyye Radiatsionnyye Porazheniya: Patogenez, Klinika, Lechenie = Combined Radiation Damage: Pathogenesis, Clinic, Treatment. Ed. Tsyba A.F., Farshatov M.N. Moscow, Meditsina Publ., 1992. 288 p. (In Russ.).
2. Silyavin S.B. Kombinirovannyye Radiatsionnyye Cherepno-Mozgovyye Porazheniya: Eksperimentalnoye Issledovaniye = Combined Radiation Craniocerebral Lesions: An Experimental Study. Extended Abstract of Candidate’s thesis in Medicine. St. Petersburg Publ., 2002. 22 p.
(In Russ.).
3. Legeza V. I., Grebenyuk A. N., Boyarintsev V. V. Kombinirovannyye Radiacionnyye Porazheniya i ih Komponenty = Combined Radiation Damage and Their Components. St. Petersburg, Foliant Publ., 2015. 216 p. ISBN 978-5-93929-254-2 (In Russ.).
4. Khoruzhenko A.F. Combined Radiation Damage in Emergency Situations of Peacetime and Wartime. Strategiya Grazhdanskoy Zashchity: Problemy i Issledovaniya. 2014;4;1:310-23 (In Russ.).
5. Khromov B.M. Kombinirovannyye Luchevyye Porazheniya = Combined Radiation Lesions. Moscow, Medgiz Publ., 1959. 343 p. (In Russ.).
6. Grebenyuk A.N., Legeza V.I., Evdokimov V.I., Saluhov V.V., Timoshevskiy A.A. Clinic, Prevention and Treatment of Radiation Damage. Part 2. Radiatsionnaya Meditsina = Radiation Medicine. Studies. Manual. Ed. Aleksanin S.S., Grebenyuk A.N. St. Petersburg Publ., 2013. 156 p. (In Russ.).
7. Torubarov F.S., Zvereva Z.F. Nevrologicheskiye Aspekty Ostroy Luchevoy Bolezni Cheloveka (Klinicheskiye Nablyudeniya) = Neurological Aspects of Acute Human Radiation Sickness (Clinical Observations). Moscow, A.I. Burnasyan FMBC Publ., 2009. 208 p. (In Russ.).
8. Byvaltsev V.A. Cherepno-Mozgovaya Travma = Traumatic Brain Injury: Textbook. Ed. Byvaltsev V.A. Kalinin A.A., et al. Irkutsk Publ., 2018. 154 p. (In Russ.).
9. Yakovlev N.A., Kargapolov A.V., Fomichev V.V., Slyusar T.A. Sposob Differencialnoy Diagnostiki Sotryaseniya Golovnogo Mozga i Ushiba Golovnogo Mozga Lyogkoy Stepeni = Method of Differential Diagnosis of Concussion and Mild Brain Injury. Patent for the Invention RU 2207572 C1, 27.06.2003. Application No. 2001133578/14 dated 10.12.2001 (In Russ.).
10. Manzhurtsev A.V., Vasyukova O.R., Menshchikov P.E., Ublinskiy M.V., Melnikov I.A., Akhadov T.A., Semenova N.A. Preliminary study of the microstructure of the brain by diffusion tensor tomography in the acute period of brain concussion. Issledovaniya i Praktika v Meditsine = Research’n Practical Medicine Journal. 2019;6;4:102-108 (In Russ.).
11. Gayvoronskaya V.I., Persichkina N.V. Diagnostic Significance of Clinical and Morphological Manifestations of Traumatic Brain Injury of Varying Severity. Problemy Ekspertizy v Meditsine = Medical Examination Problems. 2001;1;4:17-19 (In Russ.).
12. Legkaya Cherepno-Mozgovaya Travma = Mild Traumatic Brain Injury. Clinical Recommendations. Moscow Publ., 2016. 23 p. (In Russ.).
13. Karakulova Yu.V., Selyanina N. V., Eroshina O.A. Quality of Life of Patients in the Acute Period of Traumatic Brain Injury Under the Influence of Neurotrophic Therapy. Byulleten Sibirskoy Meditsiny = Bulletin of Siberian Medicine. 2011;10;2:122-126 (In Russ.).
14. Drozdova E.A., Zakharov V. V. Comparative Assessment of Cognitive Impairment in the Acute Period of Traumatic Brain Injury of Mild and Moderate Severity. Nevrologicheskiy Zhurnal = Neurological Journal. 2012;6:12-18 (In Russ.).
15. Lebedev V.V., Krylov V.V., Martynenko A.V. Problems of Computed Tomographic Classification of Brain Contusions. Almanakh Klinicheskoy Meditsiny = Almanac of Clinical Medicine. 2001;4:90-92
(In Russ.).
16. Kitayev V.M. Kitayev S.V. Luchevaya Diagnostika Zabolevaniy Golovnogo Mozga = Radiation Diagnostics of Brain Diseases. Moscow MEDpress-Inform Publ., 2015. 136 p. (In Russ.).
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.01.2022. Accepted for publication: 25.02.2023.