JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.
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The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.
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The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Issues journals
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 2
DOI:10.33266/1024-6177-2023-68-2-5-10
Lina Alhaddad1,2, Andreyan N. Osipov1,3, Sergey Leonov1,4
Radiation-Induced Premature Senescence of Tumor Cells
1School of Biological and Medical Physics, Moscow Institute of Physics and Technology, Dolgoprudny, Russia
2Department of Environmental Sciences, Faculty of Science, Damascus University, Damascus, Syria
3A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
4 Institute of Cell Biophysics, Pushchino, Russia
Contact person: Andreyan N. Osipov, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
CONTENTS
Introduction
Factors and mechanisms of Stress-Associated Secretory Phenotype (SASP)
Morphological and transcriptional signatures of SASP
Radiation-induced signaling pathways associated with premature senescence
Conclusion
Keywords: ionizing radiation, premature senescence, stress, tumor cells
For citation: Alhaddad L, Osipov AN, Leonov S. Radiation-Induced Premature Senescence of Tumor Cells. Medical Radiology and Radiation Safety. 2023;68(2):5–10. (In Russian). DOI: 10.33266/1024-6177-2023-68-2-5-10
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was carried out with the financial support of RFBR grant No. 20-34-90035.
Contribution. L. Alkhaddad – collection and analysis of literary material, writing the text. A.N. Osipov and S.V. Leonov – concept development and scientific editing.
Article received: 20.11.2022. Accepted for publication: 25.01.2023.
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 2
DOI: 10.33266/1024-6177-2023-68-2-16-20
Yu.B. Deshevoi, T.A. Nasonova, O.A. Dobrynina, V.G. Lebedev,
T.A. Astrelina, A.S. Samoylov
The Effect of Drugs for Normalize Blood Supply and Trophism of Irradiated Tissues, as well as a Broad-Spectrum Antibiotic on the Course of Severe Local Radiation Injuries in Rats
A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: Yu.B. Deshevoi, e-mail:
This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To study in the experiment the therapeutic efficacy of drugs that normalize blood supply and trophism of irradiated tissues, as well as a complex broad-spectrum antibiotic, for the course of severe local radiation lesions to justify the clinical use of these drugs in this pathology.
Material and methods: Wistar-Kyoto inbred rats were exposed to local X-rays in the ilio-lumbar region of the back at a dose of 110 Gy (tube voltage 30 kV, current 6.1 mA, Al filter 0.1 mm thick), at a dose rate of 20.1 Gy / min. Irradiation field area was 8.5 cm2. Radiation exposure caused the formation of long-term (up to 3.5–4 months) non-healing radiation ulcers of the skin without a critical radiation load on the underlying tissues. For the treatment of radiation lesions, an antibiotic was used – levotetrasulfin forte, as well as drugs that affect the blood supply and trophism of irradiated tissues – pentoxifylline and detralex. The drugs were administered daily both in isolation from each other and together during the period (from the 21st to the 42nd or from the 28th to the 48th day after irradiation), when the radiation ulcer formed and its gradual healing began. The everity of radiation skin lesions and the effects of therapy were assessed in dynamics by clinical manifestations and using planimetry.
Results: It was found that the separate use of drugs in the treatment of severe local radiation lesions was not very effective. However, with the combined administration of drugs, a noticeable increase in the rate of healing of radiation ulcers was revealed. Thus, it was shown that with the joint administration of pentoxifylline (intraperitoneal, 50.0 mg / kg), detralex (75.0 mg / kg, per os) and levotetrasulfin forte (intraperitoneal, 0.1 ml / kg), the area of radiation ulcers in treated animals in the period from 98 to 126 days after irradiation was 26–80 % less compared to irradiated controls.
Conclusion: The experiments show the possibility of successful use of a complex antibiotic in combination with drugs that improve blood supply and trophism of irradiated tissues, in the treatment of severe local radiation lesions.
Keywords: local irradiation, radiation ulcer of the skin, drug therapy, antibiotic, pentoxifylline, detralex, rats
For citation: Deshevoi YuB, Nasonova TA, Dobrynina OA, Lebedev VG, Astrelina TA, Samoylov AS. The Effect of Drugs for Normalize Blood Supply and Trophism of Irradiated Tissues, as well as a Broad-Spectrum Antibiotic on the Course of Severe Local Radiation Injuries in Rats. Medical Radiology and Radiation Safety. 2023;68(2):16–20. (In Russian). DOI: 10.33266/1024-6177-2023-68-2-16-20
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.11.2022. Accepted for publication: 25.01.2023.
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 2
DOI: 10.33266/1024-6177-2023-68-2-21-28
A.V. Rodina1, O.V. Vysotskaya1, A.S. Zhirnik1, O.D. Smirnova1, A.A. Parfenova1,
A.N. Strepetov1, Yu.P. Semochkina1, M.V. Nesterenko2, E.Yu. Moskaleva1
Features of Brain Damage after Gamma-Neutron Irradiation
of the Head and Modification of the Damage by Lactoferrin
National Research Center “Kurchatov Institute”, Moscow, Russia
LLC “Laktobio” , Moscow, Russia
Contact person: E.Yu. Moskaleva, e-mail:
This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To investigate the effect of γ, n-irradiation of the mice head on the brain cells damage, behavior and cognition, and to examine the possibility of using lactoferrin (LF) to alleviate radiation-induced impairments.
Material and methods: Mice heads were irradiated in a beam of neutrons and gamma rays from the IR-8 nuclear reactor. The brain cells of control and irradiated mice were isolated using Percoll. Neurons and resting and activated microglia cells were analyzed using the fluorescently labeled antibodies and flow cytometry. The level of DNA double-strand breaks in neurons was determined by γH2AX histone content. Cytokine gene expression in the hippocampus was studied by RT-PCR. Behavior and cognitive functions were studied using the open field, Morris water maze and novel object recognition tests. LF was isolated from female colostrum by preparative ion-exchange chromatography and purified by affinity chromatography on heparin-sepharose.
Results: γ, n-Irradiation of the mice head at a dose of 1.5 Gy led to an increase in the level of DNA double-strand breaks in neurons. Twenty-four hours after irradiation the total number of cells and the number of neurons in the isolated fraction of brain cells decreased, but the number of microglial cells remained unchanged. The number of resting and activated microglia did not change within 3–72 h after γ, n-irradiation. The expression level of the TNFα, IL-1β, and IL-6 genes increased 2 months after γ, n-irradiation of the mice head at a dose of 1.5 Gy, indicating the development of neuroinflammation. At this time, irradiated mice demonstrated the anxiety-like behavior and impaired spatial and recognition memory. A single i.p. administration of human LF to mice immediately after γ, n-irradiation of the head did not affect the observed radiation-induced disturbances, but decreased the gene expression levels of TNFα, IL-1β and IL-6 pro-inflammatory cytokines and increased the gene expression level of TGFβ anti-inflammatory cytokine in the hippocampus 2 months after radiation exposure. The obtained results indicate a partial decrease in the level of hippocampal neuroinflammation of irradiated animals treated with LF.
Conclusion: γ, n-Irradiation of the mice head at a dose of 1.5 Gy leads to DNA damage of neurons and the decrease in the number of neurons. Microglia cells are more resistant to such radiation exposure. Late after head-only γ, n-irradiation, mice develop neuroinflammation, which is detected by an increase in the pro-inflammatory cytokine gene expression in the hippocampus and also by anxiety-like behavior and impaired cognitive functions. A single LF administration leads to a partial decrease in the neuroinflammation level, but does not affect the other studied parameters. The optimal dosing regimen of LF remains to be determined to preserve cognitive functions after γ, n-irradiation of the brain.
Keywords: brain, neurons, microglia, activated microglia, double-strand breaks, DNA, neutrons, photons, local irradiation, mice, lactoferrin
For citation: Rodina AV, Vysotskaya OV, Zhirnik AS, Smirnova OD, Parfenova AA, Strepetov AN, Semochkina YuP, Nesterenko MV, Moskaleva EYu. Features of Brain Damage after Gamma-Neutron Irradiation of the Head and Modification of the Damage by Lactoferrin. Medical Radiology and Radiation Safety. 2023;68(2):21–28. (In Russian). DOI: 10.33266/1024-6177-2023-68-2-21-28
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12. Rodina A.V., Semochkina Y.P., Vysotskaya O.V., Romantsova A.N., Strepetov A.N., Moskaleva E.Y. Low Dose Gamma Irradiation Pretreatment Modulates the Sensitivity of CNS to Subsequent Mixed Gamma and Neutron Irradiation of the Mouse Head. Int. J. Radiat. Biol. 2021;97;7:926–942. DOI: 10.1080/09553002.2021.1928787.
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. National Research Center ‟Kurchatov Institute”.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.11.2022. Accepted for publication: 25.01.2023.
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 2
DOI: 10.33266/1024-6177-2023-68-2-11-15
N.Yu. Vorobyeva1,2, T.A. Astrelina1, E.I. Yashkina1,2, A.K. Chigasova3,
A.A. Osipov2, D.Yu. Usupzhanova1, I.V. Kobzeva1, Yu.B. Suchkova1,
V.A. Brunchukov1, A.A. Rastorgueva1, Yu.A. Fedotov1,2, A.S. Samoilov1,
A.N. Osipov1,2
Effect of a Humic-Fulvic Acid Preparation on the Quantitative Yield of Residual γH2AX Foci and Proliferative Activity in Irradiated Human Mesenchymal Stromal Cells
1 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2 N.N. Semenov Federal Research Center for Chemical Physics, Moscow, Russia
3 Institute of Biochemical Physics, Moscow, Russia
Contact person: N.Yu. Vorobyeva, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To evaluate the influence of a humic-fulvic acid substance on the quantitative yield of residual foci of the DNA double-strand break (DSB) repair protein-marker - phosphorylated histone H2AX (γH2AX) and proliferation activity in a culture of human mesenchymal stromal cells (MSCs) 24, 48, and 72 h after exposure to X-ray radiation at doses of 2, 4 and 10 Gy.
Material and methods: Through 24 hours after incubation of MSCs with a substance of humic-fulvic acids (Humic Complex, OOO Sistema-BioTechnologies, Russia) at a dilution of 1/1000. Cells were irradiated on an X-ray biological device RUB RUST-M1 at a voltage of 200 kV, beam current 2×5 mA, aluminum filter 1.5 mm, absorbed dose rate 0.85 Gy/min. Immunocytochemical staining was used to quantify the residual γH2AX foci and the percentage of proliferating cells using antibodies to γH2AX and Ki-67 (a marker protein for cell proliferation), respectively. Statistical analysis of the obtained data was carried out using the statistical software package Statistica 8.0 (StatSoft). To assess the significance of differences between samples, Student’s t-test was used.
Results and conclusion: The conducted studies showed that on the cell model used and under the above experimental conditions, the humic-fulvic acid substance does not affect the efficiency of repair of radiation-induced DNA DSBs, however, it significantly reduces the proliferation activity of both irradiated and non-irradiated MSCs. It is advisable to conduct detailed studies of the molecular and cellular mechanisms of the antiproliferative effect of humic and fulvic acids.
Keywords: mesenchymal stromal cells, X-ray radiation, γH2AX, residual foci, DNA double-strand breaks, cell proliferation, humic acids, fulvic acids
For citation: Vorobyeva NYu, Astrelina TA, Yashkina EI, Chigasova AK, Osipov AA, Usupzhanova DYu, Kobzeva IV, Suchkova YuB, Brunchukov VA, Rastorgueva AA, Fedotov YuA, Samoilov AS, Osipov AN. Effect of a Humic-Fulvic Acid Preparation on the Quantitative Yield of Residual γH2AX Foci and Proliferative Activity in Irradiated Human Mesenchymal Stromal Cells. Medical Radiology and Radiation Safety. 2023;68(2):11–15. (In Russian). DOI: 10.33266/1024-6177-2023-68-2-11-15
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The analysis of residual foci was carried out with the support of the RNF (project No. 22-2400490).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.11.2022. Accepted for publication: 25.01.2023.
Medical Radiology and Radiation Safety. 2023. Vol. 68. № 2
DOI: 10.33266/1024-6177-2023-68-2-29-34
A.F. Bobrov1, T.M. Novikova2, V.I. Sedin1, L.I. Fortunatova1
System Criteria for Differential Express Diagnostics of Prenosological Disorders of Occupational Health of Employees
at Nuclear Power Facilities
1A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2Central Medical and Sanitary Unit No. 91, Lesnoy, Russia
Contact person: A.F. Bobrov, e-mail:
This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: Development of complex criteria of differential express-diagnostics of pre-dosological disorders of occupational health of employees of nuclear facilities.
Material and methods: The study object was male workers of the main production facilities of the Electrohimpribor Combine undergoing periodic medical examinations and psychophysiological examinations. The state of health was assessed according to the employee’s belonging to the dispensary observation group in accordance with Order No. 404n of the Ministry of Health of the Russian Federation of April 27, 2021. The psychophysiological state was assessed using a hardware-software complex for group psychophysiological examination APK PFS-CONTROL. Additional testing techniques included assessment of vibraimage parameters, evaluated using computer programs HealthTest and VibraMI, developed in ELSIS (St. Petersburg). Totally 943 man-surveys were carried out with different combination of testing methods. The average age of the workers was (42.0 ± 1.7) years, total work experience was (23 ± 1.3) years, and work experience in the specialty was (13.0 ± 1.8) years.
Results: As a criteria of prenosological health disorders we used the characteristics of the worker’s adaptation to the factors of life activity. Adaptation disorders are evaluated according to three specific criteria: 1) assessment of the functional state of the cardiovascular system according to HRV data; 2) rapid assessment of the psychophysiological state according to vibraimage parameters; 3) assessment of personal and special abilities according to multiple intelligence assessment. For each of the criteria a “traffic light” of states and decisive rules of their formalized identification were developed. Characteristics of unfavorable states are given. Complex express-diagnostics of pre-dosological disorders of occupational health is carried out using integral index, which is a weighted sum of private “traffic light” indexes with a ball score. The formalized express-diagnostics of premalignant disorders is carried out using linear discriminant functions with an average recognition accuracy of 96.4 % or a probabilistic nomogram, which allows to graphically estimate the risk level of health disorders.
Conclusion: Improvement of medical-psychophysiological support of employees of nuclear facilities is connected with introduction of methods and criteria of differential express-diagnosis of prenosological disorders of health. The developed criteria of express-diagnostics of pre-dosological disorders of occupational health expand the existing methodological and criteria base of periodic medical examinations of nuclear industry workers, strengthen their preventive orientation with the purpose of timely application of medical, psychophysiological, organizational and other measures on preservation and maintenance of occupational health of personnel.
Keywords: nuclear industry workers, prenosological conditions, rapid diagnostics, vibraimage technology, multiple intelligence, cardiovascular system, activity index of regulatory systems
For citation: Bobrov AF, Novikova TM, Sedin VI, Fortunatova LI.System Criteria for Differential Express Diagnostics of Prenosological Disorders of Occupational Health of Employees at Nuclear Power Facilities. Medical Radiology and Radiation Safety. 2023;68(2):
29–34. (In Russian). DOI: 10.33266/1024-6177-2023-68-2-29-34
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Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.11.2022. Accepted for publication: 25.01.2023.