JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.
Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.
The journal is included in the List of Russian Reviewed Scientific Journals of the Higher Attestation Commission. Since 2008 the journal has been available on the Internet and indexed in the RISC database which is placed on Web of Science. Since February 2nd, 2018, the journal "Medical Radiology and Radiation Safety" has been indexed in the SCOPUS abstract and citation database.
Brief electronic versions of the Journal have been publicly available since 2005 on the website of the Medical Radiology and Radiation Safety Journal: http://www.medradiol.ru. Since 2011, all issues of the journal as a whole are publicly available, and since 2016 - full-text versions of scientific articles. Since 2005, subscribers can purchase full versions of other articles of any issue only through the National Electronic Library. The editor of the Medical Radiology and Radiation Safety Journal in accordance with the National Electronic Library agreement has been providing the Library with all its production since 2005 until now.
The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.
Since 2017 the journal Medical Radiology and Radiation Safety has switched to digital identification of publications, assigning to each article the identifier of the digital object (DOI), which greatly accelerated the search for the location of the article on the Internet. In future it is planned to publish the English-language version of the journal Medical Radiology and Radiation Safety for its development. In order to obtain information about the publication activity of the journal in March 2015, a counter of readers' references to the materials posted on the site from 2005 to the present which is placed on the journal's website. During 2015 - 2016 years on average there were no more than 100-170 handlings per day. Publication of a number of articles, as well as electronic versions of profile monographs and collections in the public domain, dramatically increased the number of handlings to the journal's website to 500 - 800 per day, and the total number of visits to the site at the end of 2017 was more than 230.000.
The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Issues journals
Medical Radiology and Radiation Safety. 2024. Vol. 69. № 1
DOI:10.33266/1024-6177-2024-69-1-15-19
A.K. Chigasova1, 2, 3, M.V. Pustovalova1, 4, A.A. Osipov2, S.A. Korneva5,
P.S. Eremin6, E.I. Yashkina1, 2, M.A. Ignatov1, 2,Yu.A. Fedotov1, 2,
N.Yu. Vorobyeva1, 2, A.N. Osipov1, 2
Post-Radiation Changes in The Number of Phosphorylated H2ax
and Atm Protein Foci in Low Dose X-Ray Irradiated Human Mesenchymal Stem Cells
1 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2 N.N. Semenov Federal Research Center for Chemical Physics, Moscow, Russia
3 Institute of Biochemical Physics, Moscow, Russia
4 Moscow Institute of Physics and Technology, Moscow region, Dolgoprudny, Russia
5 M.V. Lomonosov Moscow State University, Moscow, Russia
6 National Medical Research Center of Rehabilitation and Balneology, Moscow, Russia
Contact person: N.Yu. Vorobyeva, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Aim: To study the patterns of changes in the number of foci of phosphorylated DNA double-strand break repair proteins H2AX (γH2AX) and ATM (pATM) in cultured human mesenchymal stem cells (MSCs) 1‒48 hours after exposure to X-ray radiation at doses of 40, 80, 160 and 250 mGy.
Material and methods: We used the primary culture of human MSCs, obtained from the collection of LLC “BioloT” (Russia). Cells were irradiated using a RUB RUST-M1 X-ray biological unit (Diagnostika-M LLC, Moscow, Russia) equipped with two X-ray emitters at a dose rate of 40 mGy/min (voltage of 100 kV, an anode current of 8 mA, and a 1.5 mm Al filter) and 4 °C temperature. To quantify the yield of γH2AX and pATM foci immunocytochemical staining was carried out with the use of γH2AX and pATM antibody respectively. Statistical analysis of the obtained data was carried out using the statistical software package Statistica 8.0 (StatSoft). To assess the significance of differences between samples, Student’s t-test was used.
Results: It was shown that the kinetics of changes in the number of γH2AX foci after irradiation at doses of 160 and 250 mGy and low (40‒80 mGy) doses are significantly different. In contrast to the significant (50‒60 %) decrease in the number of γH2AX foci observed
6 hours after irradiation at doses of 160 and 250 mGy, after irradiation at low doses, no significant decrease in γH2AX foci was observed at this time point. Analysis of the colocalization of γH2AX foci with pATM foci indicates that the mechanisms for maintaining a high number of γH2AX foci 24‒48 hours after low-dose irradiation are ATM independent. A hypothesis has been put forward to explain the phenomenon of maintaining the number of γH2AX foci 24‒48 hours after irradiation in low doses by replicative stress caused by stimulation of proliferation against the background of hyperproduction of free radicals, resulting in additional formation of DNA double-strand breaks and phosphorylation of H2AX by ATR kinase.
Keywords: mesenchymal stem cells, γH2AX, pATM, DNA double-strand breaks, X-ray radiation, low doses
For citation: Chigasova AK, Pustovalova MV, Osipov AA, Korneva SA, Eremin PS, Yashkina EI, Ignatov MA, Fedotov YuA, Vorobyeva NYu, Osipov AN. Post-Radiation Changes in The Number of Phosphorylated H2ax and Atm Protein Foci in Low Dose X-Ray Irradiated Human Mesenchymal Stem Cells. Medical Radiology and Radiation Safety. 2024;69(1):15–19. (In Russian). DOI:10.33266/1024-6177-2024-69-1-15-19
References
1. Mastrolia I., Foppiani E.M., Murgia A., Candini O., Samarelli A.V., Grisendi G., et al. Challenges in Clinical Development of Mesenchymal Stromal/Stem Cells: Concise Review. Stem. Cells. Transl. Med. 2019;8;11:1135-1148. doi: 10.1002/sctm.19-0044.
2. Andrzejewska A., Lukomska B., Janowski M. Concise Review: Mesenchymal Stem Cells: From Roots to Boost. Stem. Cells. 2019;37;7:855-864. doi: 10.1002/stem.3016.
3. Smolinska A., Bzinkowska A., Rybkowska P., Chodkowska M., Sarnowska A. Promising Markers in the Context of Mesenchymal Stem/Stromal Cells Subpopulations with Unique Properties. Stem. Cells. Int. 2023;2023:1842958. doi: 10.1155/2023/1842958.
4. Zuk P.A., Zhu M., Mizuno H., Huang J., Futrell J.W., Katz A.J., et al. Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based Therapies. Tissue Engineering. 2001;7;2:211-228. doi: 10.1089/107632701300062859.
5. Oswald J., Boxberger S., Jorgensen B., Feldmann S., Ehninger G., Bornhauser M., et al. Mesenchymal Stem Cells Can Be Differentiated into Endothelial Cells in Vitro. Stem. Cells. 2004;22;3:377-84. doi: 10.1634/stemcells.22-3-377.
6. Пустовалова М.В., Грехова А.К., Осипов А.Н. Мезенхимальные стволовые клетки: эффекты воздействия ионизирующего излучения в малых дозах // Радиационная биология. Радиоэкология. 2018. Т.58, № 4. С. 352-362. doi: 10.1134/s086980311804015x. [Pustovalova M.V., Grekhova A.K., Osipov A.N. Mesenchymal Stem Cells: Effects of Exposure to Ionizing Radiation in Low Doses. Radiatsionnaya Biologiya. Radioekologiya = Radiation Biology. Radioecology. 2018;58;4:352-362. doi: 10.1134/s086980311804015x (In Russ.)].
7. Bushmanov A., Vorobyeva N., Molodtsova D., Osipov A.N. Utilization of DNA Double-Strand Breaks for Biodosimetry of Ionizing Radiation Exposure. Environmental Advances. 2022;8. doi: 10.1016/j.envadv.2022.100207.
8. Osipov A., Chigasova A., Yashkina E., Ignatov M., Fedotov Y., Molodtsova D., et al. Residual Foci of DNA Damage Response Proteins in Relation to Cellular Senescence and Autophagy in X-Ray Irradiated Fibroblasts. Cells. 2023;12;8. doi: 10.3390/cells12081209.
9. Belov O., Chigasova A., Pustovalova M., Osipov A., Eremin P., Vorobyeva N., et al. Dose-Dependent Shift in Relative Contribution of Homologous Recombination to DNA Repair after Low-LET Ionizing Radiation Exposure: Empirical Evidence and Numerical Simulation. Curr. Issues Mol. Biol. 2023;45;9:7352-73. doi: 10.3390/cimb45090465.
10. Georgoulis A., Vorgias C., Chrousos G., Rogakou E. Genome Instability and γH2AX. International Journal of Molecular Sciences. 2017;18;9. doi: 10.3390/ijms18091979.
11. Burma S., Chen B.P., Murphy M., Kurimasa A., Chen D.J. ATM Phosphorylates Histone H2AX in Response to DNA Double-Strand Breaks. J. Biol. Chem. 2001;276;45:42462-7. doi: 10.1074/jbc.C100466200.
12. Stiff T., O’Driscoll M., Rief N., Iwabuchi K., Lobrich M., Jeggo P.A. ATM and DNA-PK Function Redundantly to Phosphorylate H2AX after Exposure to Ionizing Radiation. Cancer Res. 2004;64;7:2390-6.
13. Zhou B.B., Elledge S.J. The DNA Damage Response: Putting Checkpoints in Perspective. Nature. 2000;408;6811:433-439. doi: 10.1038/35044005.
14. O’Driscoll M., Ruiz-Perez V.L., Woods C.G., Jeggo P.A., Goodship J.A. A Splicing Mutation Affecting Expression of Ataxia-Telangiectasia and Rad3-Related Protein (Atr) Results in Seckel Syndrome. Nature Genetics. 2003;33;4:497-501. doi: 10.1038/ng1129.
15. Reitsema T., Klokov D., Banath J.P., Olive P.L. DNA-PK Is Responsible for Enhanced Phosphorylation of Histone H2AX under Hypertonic Conditions. DNA Repair (Amst). 2005;4;10:1172-1181. doi: 10.1016/j.dnarep.2005.06.005.
16. Shibata A., Jeggo P.A. ATM’s Role in the Repair of DNA Double-Strand Breaks. Genes. 2021;12;9. doi: 10.3390/genes12091370.
17. Lee J.H., Paull T.T. Activation and Regulation of ATM Kinase Activity in Response to DNA Double-Strand Breaks. Oncogene. 2007;26;56:7741-7748. doi: 10.1038/sj.onc.1210872.
18. Kurz E.U., Lees-Miller S.P. DNA Damage-Induced Activation of ATM and ATM-Dependent Signaling Pathways. DNA Repair (Amst). 2004;3;8-9:889-900. doi: 10.1016/j.dnarep.2004.03.029.
19. Osipov A.N., Pustovalova M., Grekhova A., Eremin P., Vorobyova N., Pulin A., et al. Low Doses of X-Rays Induce Prolonged and ATM-Independent Persistence of GammaH2AX foci in Human Gingival Mesenchymal Stem Cells. Oncotarget. 2015;6;29:27275-87. doi: 10.18632/oncotarget.4739.
20. Грехова А.К., Еремин П.С., Осипов А.Н., Еремин И.И., Пустовалова М.В., Озеров И.В. и др. Замедленные процессы образования и деградации фокусов γН2ax в фибробластах кожи человека, подвергшихся воздействию рентгеновского излучения в малых дозах // Радиационная биология Радиоэкология. 2015;55(4):395-401. doi: 10.7868/s0869803115040037. [Grekhova A.K., Eremin P.S., Osipov A.N., Eremin I.I., Pustovalova M.V., Ozerov I.V., et al. Slow Processes of Formation and Degradation of γH2ax Foci in Human Skin Fibroblasts Exposed to Low-Dose X-Ray Radiation. Radiatsionnaya Biologiya. Radioekologiya = Radiation Biology. Radioecology. 2015;55;4:395-401. doi: 10.7868/s0869803115040037. (In Russ.)].
21. Biswas H., Makinwa Y., Zou Y. Novel Cellular Functions of ATR for Therapeutic Targeting: Embryogenesis to Tumorigenesis. International Journal of Molecular Sciences. 2023;24;14. doi: 10.3390/ijms241411684.
22. Suzuki K., Okada H., Yamauchi M., Oka Y., Kodama S., Watanabe M. Qualitative and Quantitative Analysis of Phosphorylated ATM Foci Induced by Low-Dose Ionizing Radiation. Radiat Res. 2006;165;5:499-504. doi: 10.1667/RR3542.1.
23. Large M., Reichert S., Hehlgans S., Fournier C., Rodel C., Rodel F. A Non-Linear Detection of Phospho-Histone H2AX in EA.hy926 Endothelial Cells Following Low-Dose X-Irradiation Is Modulated by Reactive Oxygen Species. Radiat Oncol. 2014;9:80. doi: 10.1186/1748-717X-9-80.
24. Baulch J.E., Craver B.M., Tran K.K., Yu L., Chmielewski N., Allen B.D., et al. Persistent Oxidative Stress in Human Neural Stem Cells Exposed to Low Fluences of Charged Particles. Redox Biology. 2015;5:24-32. doi: 10.1016/j.redox.2015.03.001.
25. Liang X., So Y.H., Cui J., Ma K., Xu X., Zhao Y., et al. The Low-Dose Ionizing Radiation Stimulates Cell Proliferation Via Activation of the MAPK/ERK Pathway in Rat Cultured Mesenchymal Stem Cells. Journal of Radiation Research. 2011;52;3:380-386.
26. Petermann E., Helleday T. Pathways of Mammalian Replication Fork Restart. Nature Reviews Molecular Cell Biology. 2010;11;10:683-687. doi: 10.1038/nrm2974.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The research was carried out with the support of the RNF (project No. 23-14-00078).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.10.2023. Accepted for publication: 27.11.2023.
Medical Radiology and Radiation Safety. 2024. Vol. 69. № 1
DOI:10.33266/1024-6177-2024-69-1-20-27
V.A. Anikina 1, S.S. Sorokina 1, A.E. Shemyakov 1,2, E.A. Zamyatina 1,
N.R. Popova 1
Comparative Assessment of the Effect of Local Proton Radiation
with a Dose of 30 Gy in BALB/c and C57BL/6 Mice
1 Institute of Theoretical and Experimental Biophysics, Moscow Region, Pushchino, Russia
2 Branch “Physio-Technical Center” of the P.N. Lebedev Physical Institute, Moscow Region, Protvino, Russia
Contact person: N.R. Popova, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To evaluate the effect of local proton irradiation at a dose of 30 Gy on Balb/c and C57BL/6 mice in terms of the degree and dynamics of radiation-induced skin damage formation, changes in body weight and peripheral blood elements count.
Material and methods: Experiments were performed on non-depilated male mice aged 7‒8 weeks from two strains: Balb/c and C57BL/6 (n=15). Local irradiation of the skin was carried out on the dorsal side of the animals using a scanning proton beam at an extended Bragg peak in the proton therapy complex «Prometheus» of the LPI Physico-technical Centre (Protvino) at a dose of 30 Gy with a proton energy of 87.8 MeV. During the irradiation session, animals were subjected to intraperitoneal anesthesia using a combination of Zoletil 100 (Virbac, France) and Xyla (Interchemie, Netherlands) in a previously determined ratio 1:3 (20‒40 mg/kg). Photographic documentation of radiation-induced skin damage was performed weekly for 70 days. Animals were examined daily for clinical manifestations of radiation-induced skin damage formation according to the RTOG international scale for 21 days following irradiation. The body weight dynamics of mice were evaluated one day before irradiation and then weekly for 70 days. Blood samples were collected from the tail vein by cutting the tip of the tail and analyzed using a DH36 Vet hematology analyzer (Dymind, China) one day before irradiation, one day and three days after irradiation, and weekly thereafter for 70 days. Experimental data were presented as mean ± standard deviation (M ± SD).
Results: In this study, the impact of a single local exposure to proton radiation at a dose of 30 Gy on the degree and dynamics of radiation-induced skin damage formation was evaluated. It was demonstrated that Balb/c mice exhibited a higher frequency and degree of radiation-induced skin damage formation compared to the C57BL/6 mice. Analysis of body weight in mice after radiation exposure revealed no significant decrease in either mouse strain. A comparative analysis of the number of platelets, erythrocytes and hemoglobin concentration in both mouse strains did not reveal any changes, while a tendency towards a decrease in the number of leukocytes, lymphocytes, and granulocytes was observed in the irradiated Balb/c mice group compared to the control group. Conversely, in irradiated C57BL/6 mice, the number of lymphocytes was higher compared to control animals.
Conclusion: In this study, Balb/c mice exhibited higher radiosensitivity compared to C57BL mice in response to a single local proton irradiation at a dose of 30 Gy.
Keywords: proton radiation, radiation dermatitis, radiation burn, hematological analysis, BALB/c and C57BL/6 mice
For citation: Anikina VA, Sorokina SS, Shemyakov AE, Zamyatina EA, Popova NR. Comparative Assessment of the Effect of Local Proton Radiation with a Dose of 30 Gy in BALB/c and C57BL/6 Mice. Medical Radiology and Radiation Safety. 2024;69(1):20–27. (In Russian). DOI:10.33266/1024-6177-2024-69-1-20-27
References
1. Anikina V.A., Sorokina S.S., Shemyakov A.E., Taskaeva Iu.S., Zamyatina E.A., Teplova P.O., Popova N.R. First Experimental Model of Proton Beam-Induced Radiation Dermatitis in Vivo. Int. J. Mol. Sci. 2023;24;22:16373.
2. Cox J.D., Stetz J., Pajak T.F. Toxicity criteria of the Radiation Therapy Oncology Group (RTOG) and the European Organization for Research and Treatment of Cancer (EORTC). Int. J. Radiat. Oncol. Biol. Phys. 1995;31;5:1341–1346.
3. Venkatakrishnan P., Kumar G., Sampadarao B. Study of the Various Cutaneous Adverse Reactions to Radiotherapy. International Journal of Research in Dermatology. 2021;7:250.
4. Grebenyuk A.N., Legeza V.I., Zargarova N.I., Vladimirova O.O. Invention RF №RU2534802C1.2013 (In Russ.).
5. Park J.-H., Byun H.J., Kim H.J., Oh S.J., Choi C., Noh J.M, Oh D., Lee J-H., Lee D-Y. Effect of Photobiomodulation Therapy On Radiodermatitis In A Mouse Model: An Experimental Animal Study. Lasers Med. Sci. 2021;36;4:843–853.
6. Yang K., Kim S.-Y., Park J.-H., Ahn W.-G., Jung S.H., Oh D., Park H.C., Choi C. Topical Application of Phlorotannins from Brown Seaweed Mitigates Radiation Dermatitis in a Mouse Model. Mar Drugs. 2020;18;8:377.
7. Janko M., Ontiveros F., Fitzgerald T.J., Deng A., DeCicco M., Rock K.L. IL-1 Generated Subsequent to Radiation-Induced Tissue Injury Contributes to the Pathogenesis of Radiodermatitis. Radiat. Res. 2012;178;3:166–172.
8. Flanders K.C., Major C.D., Arabshahi A., Aburime E.E., Okada M.H., Fujii M., Blalock T.D., Schultz G.S., Sowers A., Anzano M.A., Mitchell J.B., Russo A., Roberts A.B. Interference with Transforming Growth Factor-β/ Smad3 Signaling Results in Accelerated Healing of Wounds in Previously Irradiated Skin. Am. J. Pathol. 2003;163;6:2247–2257.
9. Koch A., Gulani J., King G., Hieber K., Chappell M., Ossetrova N. Establishment of Early Endpoints in Mouse Total-Body Irradiation Model. PLoS ONE. 2016;11;8:e0161079.
10. Gridley D.S., Pecaut M.J. Changes in the Distribution and Function of Leukocytes after Whole-Body Iron Ion Irradiation. J. Radiat. Res. 2016;57;5:477–491.
11. Kang Y.-M., Shin S.-C., Jin Y.-W., Kim H.-S. Changes in Body and Organ Weights, Hematological Parameters, and Frequency of Micronuclei in the Peripheral Blood Erythrocytes of ICR Mice Exposed to Low-Dose-Rate γ-Radiation. Journal of Radiation Protection. 2009;34;3:102-106.
12. Pecaut M.J., Dutta-Roy R., Smith A.L., Jones T.A., Nelson G.A., Gridley D.S. Acute effects of iron-particle radiation on immunity. Part I: Population Distributions. Radiat. Res. 2006;165;1:68–77.
13. Gridley D.S., Pecaut M.J., Nelson G.A. Total-Body Irradiation with High-LET Particles: Acute and Chronic Effects on the Immune System. Am. J. Physiol. Regul. Integr. Physiol. 2002;282;3:R677–R688.
14. Pecaut M.J., Gridley D.S. The Impact of Mouse Strain on Iron Ion Radio-Immune Response of Leukocyte Populations. Int. J. Radiat. Biol. 2010;86;5:409–419.
15. Gridley D.S., Obenaus A., Bateman T.A., Pecaut M.J. Long-Term Changes in Rat Hematopoietic and Other Physiological Systems after High-Energy Iron Ion Irradiation. Int. J. Radiat. Biol. 2008;84;7:549–559.
16. Stenson S. Weight Change and Mortality of Rats After Abdominal Proton and Roentgen Irradiation. A Comparative Investigation. Acta Radiol. Ther. Phys. Biol. 1969;8;5:423–432.
17. Karkischenko V.N., Schmidt E.F., Braytseva E.V. The Researchers Prefer BALB/c Mice. Biomeditsina = Journal Biomed. 2007;1:57–70 (In Russ.).
18. Shakhovskaya O.V., Starodubtseva M.N., Medvedeva A.A. Characteristics of Radiosensitivity of Organisms Using Parameters of Redox Properties of Blood Plasma. Mediko-Biologicheskiye Problemy Zhiznedeyatelnosti = Medical and Biological Problems of Life Activity. 2023;1:43-48. DOI:10.58708/2074-2088.2023-1(29)-43-48
(In Russ.).
19. Fabusheva K.M., Dvornik Yu.V. The Effect of Nicotinic Acid on the Level of Radiation-Induced DNA Damage in Mouse Bone Marrow Cells. VIII Mezhdunarodnaya Nauchno-Prakticheskaya Konferentsiya Molodyh Uchenyh: Biofizikov, Biotekhnologov, Molekulyarnyh Biologov i Virusologov – 2021 = VIII International Scientific and Practical Conference of Young Scientists: Biophysicists, Biotechnologists, Molecular Biologists and Virologists – 2021. Novosibirsk 2021, Oct 5-8. Novosibirsk Publ., 2021. P. 394-395 (In Russ.).
20. Mao X.W., Boerma M., Rodriguez D., Campbell-Beachler M., Jones T., Stanbouly S., Sridharan V., Nishiyama N.C., Wroe A., Nelson G.A. Combined Effects of Low-Dose Proton Radiation and Simulated Microgravity on the Mouse Retina and the Hematopoietic System. Radiat. Res. 2019;192;3:241–250.
21. Ware J., Sanzari J., Avery S., Sayers C., Krigsfeld G., Nuth M., Wan X.S., Kennedy A.R. Effects of Proton Radiation Dose, Dose Rate and Dose Fractionation on Hematopoietic Cells in Mice. Radiation Research. 2010;174:325–330.
22. Romero-Weaver A.L., Wan X.S., Diffenderfer E.S., Lin L., Kennedy A.R Effect of SPE-Like Proton or Photon Radiation on the Kinetics of Mouse Peripheral Blood Cells and Radiation Biological Effectiveness Determinations. Astrobiology. 2013;13;6:570–577.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study was done with the support of the RNF grant No. 22-63-00082.
Contribution. Development of the research concept: Popova N.R., Sorokina S.S.; development of the research design: Popova N.R., Sorokina S.S., Anikina V.A.; conducting experiments: Anikina V.A., Zamyatina E.A., Shemyakov A.E., development and modification of research methods: Anikina V.A., Shemyakov A.E.; collection and analysis of literary material: Anikina V.A., Sorokina S.S., Popova N.R.; statistical data processing: Anikina V.A.; writing and scientific editing of the text: Sorokina S.S., Popova N.R.
Article received: 20.10.2023. Accepted for publication: 27.11.2023.
Medical Radiology and Radiation Safety. 2024. Vol. 69. № 1
DOI:10.33266/1024-6177-2024-69-1-33-40
L.I. Baranov, A.N.Tsarev, F.S. Torubarov, A.S. Kretov, V.V. Petrova, E.Vasilyev,
S.M. Dumansky, O.A. Tikhonova, T.M. Bulanova, M.V. Kalinina, P.A. Shulepov,
I. Dibirgadzhiyev, A.S. Samoilov
Digital Twin of Worker of Nuclear Facility at the Stage of Pre-Shift Control
A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: L.I. Baranov, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Introduction. Digital twin. Digital twin in medicine using the example of Philips. Digital twin as an object of medical information space. Digital twin as an abstraction. Digital twin of worker of nuclear facility at the stage of pre-shift control. Conclusion.
Keywords: worker of nuclear facility, digital twin, pre-shift control, medical information space, abstraction
For citation: Baranov LI, Tsarev AN, Torubarov FS, Kretov AS, Petrova VV, Vasilyev EV, Dumansky SM, Tikhonova OA, Bulano-
va TM, Kalinina MV, Shulepov PA, Dibirgadzhiyev I, Samoilov AS. Digital Twin of Worker of Nuclear Facility at the Stage of Pre-Shift Control. Medical Radiology and Radiation Safety. 2024;69(1):33–40. (In Russian). DOI:10.33266/1024-6177-2024-69-1-33-40
References
1. Grieves M. Digital Twin: Manufacturing Excellence through Virtual Factory Replication. Researchgate. URL: https://www.researchgate.net/publication/275211047_Digital_Twin_Manufacturing_Excellence_through_Virtual_Factory_Replication. (Available 13.09.2022).
2. Grieves M. Intelligent Digital Twins and the Development and Management of Complex Systems [version 1; peer review: 4 approved]. URL: https://digitaltwin1.org/articles/2-8. (Available 07.08.2023).
3. Gonzalez C.M. 6 Questions with Michael Grieves on the Future of Digital Twins URL: https://www.asme.org/topics-resources/content/6-question-with-michael-grieves-on-the-future-of-digital-twins. (Available 06.07.2023).
4. Prokhorov A., Lysachev M. Borovkov A. Tsifrovoy Dvoynik. Analiz, Trendy, Mirovoy Opyt. Korporativnoye Izdaniye ROSATOM = Digital Twin. Analysis, Trends, World Experience. Corporate publication ROSATOM. Moscow Publ., 2020.
401 p. (In Russ.).
5. Grieves M. Origins of the Digital Twin Concept. URL: https://www.researchgate.net/publication/307509727_Origins_of_the_Digital_Twin_Concept. (Available 06.07.2023). DOI:10.13140/RG.2.2.26367.61609.
6. URL: https://www.redhat.com/en/resources/understanding-digital-twin-environments-detail. (Available: 10.08.2023).
7. URL: https://www.philips.com/a-w/about/news/archive/blogs/innovation-matters/20180830-the-rise-of-the-digital-twin-how-healthcare-can-benefit.html. (Available:11.07.2023).
8. URL: https://www.philips.com/a-w/about/news/archive/blogs/innovation-matters/20181112-how-a-virtual-heart-could-save-your-real-one.html. (Дата обращения:11.07.2023).
9. Zudilina N.V. About Some Reasons for the Existence of “Platonic” (“Real”, “Imaginary”) and “Aristotelian” (“Possible”, “Effective”) Meanings in which the Meaning of the Word “Virtual” Is Expressed in Russian. Vestnik Moskovskogo Universiteta. Seriya 22. Teoriya Perevoda. 2019;3 (In Russ.).
10. On Information, Information Technologies and Information Protection: Federal Law of July 27, 2006 No. 149-FZ. (In Russ.).
11. GOST 33707-2016. (ISO/IEC 2382:2015) Information Technology (IT). Dictionary. (In Russ.).
12. О Strategies for the development of the information society in the Russian Federation for 2017 - 2030: Decree of the President of the Russian Federation dated 05/09/2017 No. 203. (In Russ.).
13. What is meant by an information resource? // SPS Consultant+. Current as of 10.08.2023. (In Russ.).
14. On the Basics of Protecting the Health of Citizens in the Russian Federation: Federal Law of November 21, 2011 N 323-FZ (as Amended 24.07.2023). (In Russ.).
15. On the Security of Critical Information Infrastructure of the Russian Federation: Federal Law Dated July 26, 2017 N 187-FZ (as Amended on July 10, 2023) (In Russ.).
16. GOST R 54136-2010. Industrial Automation Systems and Integration: Standards Application Guide, Structure and Vocabulary. (In Russ.).
17. Terminological Dictionary of Automation of Construction and Production Processes. DOI 10.34660/c0727-6092-6372-a. URL: http://slovar-avt.ru/ (Available: 02.08.2023). (In Russ.).
18. Butch G., Maksimchuk R.A., Engle M.W., Young B.J., Conallen D., Houston K.A. Object-Oriented Analysis and Design with Example Applications. Moscow Publ., 2008. 720 p. (In Russ.).
19. HeartModelA.I. Removing the complexity of Live 3D Quantification. URL: https://www.philips.com/c-dam/b2bhc/master/feature-details/aius/452299111691_heartmodel_whitepaper_lr.pdf. (Available: 17.08.2023).
20. Guidelines for Conducting Medical Examinations and Psychophysiological Examinations of Workers at Nuclear Energy Facilities. Moscow Publ., 1998. 28 p. (In Russ.).
21. On Approval of Requirements for Medical Examinations and Psychophysiological Examinations of Employees of Nuclear Energy Facilities, the Procedure for Conducting Them, a List of Medical Contraindications for Issuing Permission to Perform Certain Types of Activities in the Field of Atomic Energy Use and a List of Positions of Employees of Nuclear Energy Facilities That Are Subject to These Contraindications , as Well as Forms of a Medical Report on the Presence (Absence) of Medical Contraindications for Issuing Permission to Perform Certain Types of Activities in the Field of Atomic Energy Use: Order of the Ministry of Health of Russia dated July 28, 2020 No. 749n. (In Russ.).
22. On Approval of the Procedure and Frequency of Pre-Shift, Pre-Trip, Post-Shift, Post-Trip Medical Examinations, Medical Examinations During the Working Day (Shift) and the List of Studies Included in Them: Order of the Ministry of Health of Russia dated May 30, 2023 No. 266n (In Russ.).
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.10.2023. Accepted for publication: 27.11.2023.
Medical Radiology and Radiation Safety. 2024. Vol. 69. № 1
DOI:10.33266/1024-6177-2024-69-1-28-32
L.A. Romodin1, E.I. Yashkina1, A.A. Moskovskij2
Fluorimetric Evaluation of the Effect of Malic, Succinic and Ascorbic Acids on the Growth Properties of A549 Cells in Culture
1 A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
2 Russian Biotechnological University, Moscow, Russia
Contact person: L.A. Romodin, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Relevance: A number of researchers consider the study of the radioprotective properties of non-toxic or low-toxic natural substances to be a promising direction. A special place among them is occupied by antioxidants and participants in the basic reactions of metabolism. In order to avoid methodological errors when performing these studies, it is necessary to conduct a number of additional experiments. For example, in order to study the properties of various substances on cell cultures using tablet readers, it is first necessary to make sure that these substances do not affect the ability of cells to adsorb to the bottom of the wells of the tablet and do not interfere with cell proliferation. And if such an influence is detected, further experiments with these substances should be planned taking into account the information received.
Purpose: To search the effect of ascorbic, malic and succinic acids on the ability of lung adenocarcinoma cells (A549) to adhere in a 96-well plate, followed by the onset of proliferation by fluorescence registration method using Hoechst-33342 fluorophore.
Methodology: The experiment was carried out in a 96-well tablet. The working concentration of Hoechst-33342 was 1 μg/ml (1.62 μM). Fluorescence was recorded at a wavelength of 460 nm when the samples were excited by light with a wavelength of 355 nm. In an experiment to study the effect of ascorbate, malate and succinate on cell adhesion and proliferation, 20,000 cells and a solution of one of these substances in a working concentration of 2 mM were introduced into the cells of the tablet. The number of cells in the wells was estimated based on the fluorescence of Hoechst-33342 after a day of incubation.
Result: In samples containing 2 mM succinic acid and ascorbic acid, a statistically significant decrease in the intensity of fluorescence was observed compared with a sample that did not contain the drug. This suggests that these compounds negatively affect the growth properties of the A549 culture: they inhibit cell adhesion or slow down their proliferation.
Scope of the results and conclusions:The results obtained are necessary for the methodologically correct planning of the most detailed studies on the A549 cell line model using fluorescent methods, including studies on the radioprotective properties of ascorbate, malate and succinate under the influence of rare ionizing and neutron radiation.
Keywords: cell culture, A549, ascorbic acid, succinate, malic acid, Hoechst-33342 flatbed fluorimeter, influence estimation
For citation: Romodin LA, Yashkina EI, Moskovskij AA. Fluorimetric Evaluation of the Effect of Malic, Succinic and Ascorbic Acids on the Growth Properties of A549 Cells in Culture. Medical Radiology and Radiation Safety. 2024;69(1):28–32. (In Russian). DOI:10.33266/1024-6177-2024-69-1-28-32
References
1.Rozhdestvenskiy L.M. Difficulties in Radiation Counter Measure Preparations Development in Russiain Crysis Period: Actual Approaches Searching. Radiatsionnaya Biologiya. Radioekologiya = Radiation Biology. Radioecology. 2020;60;3:279–290. doi: 10.31857/S086980312003011X (In Russ.).
2.Raj S., Manchanda R., Bhandari M., Alam M.S. Review on Natural Bioactive Products as Radioprotective Therapeutics: Present and Past Perspective. Current Pharmaceutical Biotechnology. 2022;23;14:1721–1738. doi: 10.2174/1389201023666220110104645.
3.Gonzalez E., Cruces M.P., Pimentel E., Sanchez P. Evidence that the Radioprotector Effect of Ascorbic Acid Depends on the Radiation Dose Rate. Environmental Toxicology and Pharmacology. 2018;62:210–214. doi: 10.1016/j.etap.2018.07.015.
4.Zakirova G. Sh., Ishmukhametov K.T., Saitov V.R., Kadikov I.R. The Effectiveness of the Use of Fumaric and Succinic Acids Salts in Combined Lesions of Rabbits. Vestnik Mariyskogo Gosudarstvennogo Universiteta. Seriya: Selskohozyaystvennyye Nauki. Ekonomicheskiye Nauki = Vestnik of the Mari State University. Chapter “Agriculture. Economics”. 2022;8;31:256–263. doi: 10.30914/2411-9687-2022-8-3-256-263 (In Russ.).
5.Burlakova E.B., Alesenko A.V., Molochkina E.M., Pal’mina N.P., Khrapova N.G. Bioantioksidanty v Luchevom Porazhenii i Zlokachestvennom Roste = Bioantioxidants in Radiation Damage and Malignant Growth. Moscow, Nauka Publ., 1975. 213 p. (In Russ.).
6.Kuzin A.M. Strukturno-Metabolicheskaya Teoriya v Radiobiologii = Structural and Metabolic Theory in Radiobiology. Moscow, NaukaPubl., 1986. 282 p. (In Russ.).
7.Mousavi A., Pourakbar L., Siavash Moghaddam S. Effects of Malic Acid and EDTA on Oxidative Stress and Antioxidant Enzymes of Okra (Abelmoschus Esculentus L.) Exposed to Cadmium Stress. Ecotoxicology and Environmental Safety. 2022;248:114320. doi: 10.1016/j.ecoenv.2022.114320.
8.Zeng X., Wu J., Wu Q., Zhang J. L-Malate Enhances the Gene Expression of Carried Proteins and Antioxidant Enzymes in Liver of Aged Rats. Physiological Research. 2015;64;1:71–78. doi: 10.33549/physiolres.932739.
9.Vuyyuri S.B., Rinkinen J., Worden E., Shim H., Lee S., Davis K.R. Ascorbic Acid and a Cytostatic Inhibitor of Glycolysis Synergistically Induce Apoptosis in Non-Small Cell Lung Cancer Cells. PloS One. 2013;8;6:e67081. doi: 10.1371/journal.pone.0067081.
10.Fromberg A., Gutsch D., Schulze D., Vollbracht C., Weiss G., Czubayko F., Aigner A. Ascorbate Exerts Anti-Proliferative Effects Through Cell Cycle Inhibition and Sensitizes Tumor Cells Towards Cytostatic Drugs. Cancer Chemotherapy and Pharmacology. 2011;67;5:1157–1166. doi: 10.1007/s00280-010-1418-6.
11.Reang J., Sharma P.C., Thakur V.K., Majeed J. Understanding the Therapeutic Potential of Ascorbic Acid in the Battle to Overcome Cancer. Biomolecules. 2021;11;8:1130. doi: 10.3390/biom11081130.
12.Gazivoda T., Wittine K., Lovric I., Makuc D., Plavec J., Cetina M., Mrvos-Sermek D., Suman L., Kralj M., Pavelic K., Mintas M., Raic-Malic S. Synthesis, Structural Studies, and Cytostatic Evaluation of 5,6-di-O-Modified L-Ascorbic Acid Derivatives. Carbohydrate Research. 2006;341;4:433–442. doi: 10.1016/j.carres.2005.12.010.
13.Gazivoda T., Sokcevic M., Kralj M., Suman L., Pavelic K., De Clercq E., Andrei G., Snoeck R., Balzarini J., Mintas M., Raic-Malic S. Synthesis and Antiviral and Cytostatic Evaluations of the New C-5 Substituted Pyrimidine and Furo[2,3-d]Pyrimidine 4’,5’-Didehydro-L-Ascorbic Acid Derivatives. Journal of Medicinal Chemistry. 2007;50;17:4105–4112. doi: 10.1021/jm070324z.
14.Gazivoda T., Raic-Malic S., Marjanovic M., Kralj M., Pavelic K., Balzarini J., De Clercq E., Mintas M. The Novel C-5 Aryl, Alkenyl and Alkynyl Substituted Uracil Derivatives of L-Ascorbic Acid: Synthesis, Cytostatic, and Antiviral Activity Evaluations. Bioorganic & Medicinal Chemistry. 2007;15;2:749–758. doi: 10.1016/j.bmc.2006.10.046.
15.Ertugrul B., Iplik E.S., Cakmakoglu B. In Vitro Inhibitory Effect of Succinic Acid on T-Cell Acute Lymphoblastic Leukemia Cell Lines. Archives of Medical Research. 2021;52;3:270–276. doi: 10.1016/j.arcmed.2020.10.022.
16.Kasarci G., Ertugrul B., Iplik E.S., Cakmakoglu B. The Apoptotic Efficacy of Succinic Acid on Renal Cancer Cell Lines. Medical Oncology. 2021;38;12:144. doi: 10.1007/s12032-021-01577-9.
17.Iplik E.S., Catmakas T., Cakmakoglu B. A New Target for the Treatment of Endometrium Cancer by Succinic Acid. Cellular and Molecular Biology. 2018;64;1:60–63. doi: 10.14715/cmb/2018.64.1.11.
18.Fuchs H., Jahn K., Hu X., Meister R., Binter M., Framme C. Breaking a Dogma: High-Throughput Live-Cell Imaging in Real-Time with Hoechst 33342. Advanced Healthcare Materials. 2023;12;20:e2300230. doi: 10.1002/adhm.202300230.
19.Cordeiro M.M., Filipe H.A.L., Santos P.D., Samelo J., Ramalho J.P.P., Loura L.M.S., Moreno M.J. Interaction of Hoechst 33342 with POPC Membranes at Different pH Values. Molecules. 2023;28;15:5640. doi: 10.3390/molecules28155640.
20.Oka N., Komuro A., Amano H., Dash S., Honda M., Ota K., Nishimura S., Ueda T., Akagi M., Okada H. Ascorbate Sensitizes Human Osteosarcoma Cells to the Cytostatic Effects of Cisplatin. Pharmacology Research & Perspectives. 2020;8;4:e00632. doi: 10.1002/prp2.632.
21.Jiang S.S., Xie Y.L., Xiao X.Y., Kang Z.R., Lin X.L., Zhang L., Li C.S., Qian Y., Xu P.P., Leng X.X., Wang L.W., Tu S.P., Zhong M., Zhao G., Chen J.X., Wang Z., Liu Q., Hong J., Chen H.Y., Chen Y.X., Fang J.Y. Fusobacterium Nucleatum-Derived Succinic Acid Induces Tumor Resistance to Immunotherapy in Colorectal Cancer. Cell Host & Microbe. 2023;31;5:781–797 e789. doi: 10.1016/j.chom.2023.04.010.
22.Ragab E.M., El Gamal D.M., Mohamed T.M., Khamis A.A. Therapeutic Potential of Chrysin Nanoparticle-Mediation Inhibition of Succinate Dehydrogenase and Ubiquinone Oxidoreductase in Pancreatic and Lung Adenocarcinoma. European Journal of Medical Research. 2022;27;1:172. doi: 10.1186/s40001-022-00803-y.
23.Ragab E.M., El Gamal D.M., Mohamed T.M., Khamis A.A. Impairment of Electron Transport Chain and Induction of Apoptosis by Chrysin Nanoparticles Targeting Succinate-Ubiquinone Oxidoreductase in Pancreatic and Lung Cancer Cells. Genes & Nutrition. 2023;18;1:4. doi: 10.1186/s12263-023-00723-4.
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was carried out within the framework of the research project “Technology-3” (registration number of the research project in the EGISU R&D system: 1230113001053).
Contribution. Article was prepared with equal participation of the authors.
Article received: 20.10.2023. Accepted for publication: 27.11.2023.
Medical Radiology and Radiation Safety. 2024. Vol. 69. № 1
DOI:10.33266/1024-6177-2024-69-1-41-49
A.V. Titov, Iu.S. Belskikh, D.V. Isaev, N.K. Shandala, T.A. Doroneva,
I.I. Bogdanov, M.P. Semenova, A.A. Shitova, S.L. Burthev
Radio-Ecological Situation in the Area of the Uranium Legacy Site – Stepnaya Mine (Kalmykia)
A.I. Burnazyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: A.V. Titov, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To study the radio-ecological situation on the “uranium legacy” site of the former Stepnaya mine in the Republic of Kalmykia.
Material and methods: To measure the ambient dose equivalent rate (ADER), the pedestrian gamma survey method was used using a portable spectrometric complex MKC-01A Multirad-M and dosimeter-radiometer MKC-AT6101c.
The activity of gamma-emitting radionuclides in soil samples was measured using a stationary gamma spectrometer from CANBERRA. The activities of 210Po and 210Pb were measured using a radiometric installation UMF-2000 following their radiochemical separation from samples.
Short-term measurements of activity concentration (AC) and equivalent equilibrium activity concentration (EEAC) of radon were carried out with an aerosol alpha radiometer for radon and thoron RAA-20P2 Poisk.
Doses of radiation exposure to biological objects were estimated using dose coefficients provided by ICRP Publication 136 taking into account recommendations R52.18.820-2015.
Results: Gamma ADER values at the mine site vary over the range from 0.1 to 0.36 µSv/h, and on 80 % of the area these values do not exceed the background value of 0.14 µSv/h. Along the road from the mine to Narta village the ADER values do not exceed background values with exception of the area around the dam, where in a local part of this area of about 300 m2 these values reach 0.49 µSv/h.
The specific activities of natural radionuclides in the soil are below the criteria for classification as solid radioactive waste (SRW).
Under the certain weather conditions, radon EEAC inside the buildings on the site reaches 13 kBq/m3, and on the territory 1-1.5 kBq/m3.
Ecological risk for the terrestrial biological objects under consideration (grass, soil worm, snake and mouse-like rodents) does not exceed 0.025.
Conclusions: The radiation situation at the Stepnaya mine site meets the requirements of SP LKP-91, which were in force until 2020. However, in order to transfer the facility to a local government body, reclamation work should be carried out in accordance with the Federal Law “On the Transfer of Lands or Land Plots from One Category to Another” dated December 21, 2004 No. 172-FZ and GOST R 59057— 2020 «Environmental Protection. Lands. General Requirements for Reclamation of Affected Lands».
Doses of exposure to biological objects do not impact significantly on morbidity, reproduction and life expectancy of terrestrial biological objects.
Keywords: bioobject, gamma radiation, natural radionuclides, radio-ecological survey, mine, specific activity
For citation: Titov AV, Belskikh IuS, Isaev DV, Shandala NK, Doroneva TA, Bogdanov II, Semenova MP, Shitova AA, Burthev SL. Radio-Ecological Situation in the Area of the Uranium Legacy Site – Stepnaya Mine (Kalmykia). Medical Radiology and Radiation Safety. 2024;69(1):41–49. (In Russian). DOI:10.33266/1024-6177-2024-69-1-41-49
References
1.URL: https://koka-lermont.livejournal.com/2820131.html. (Date of Access: 20.10.2023).
2.Sharkov A.A. Geological Phenomen of Uranium and Rare Metal Deposits. Priroda. 2015;2:21-30. (In Russ.).
3.Pyatov E.A. Strane Byl Nuzhen Uran. Istoriya Geologorazvedochnyh Rabot na Uran v SSSR = The Country Needed Uranium. History of Geological Exploration for Uranium in the USSR. Ed. Mashkovtsev G.A. Moscow Publ., 2005. 246. (In Russ.).
4.URL: https://www.rosnedra.gov.ru/data/Fast/Files/202011/6b230b8651203abb9ea69156ba246bc4.pdf. Microsoft Word - _MSB_KALMYKIYA_15.03.2022.docx (vsegei.ru). (Date of Access: 20.10.2023) (In Russ.)
5.URL: http://www.conventions.ru/view_base.php?id=9680 (Adopted in Moscow on 27.12.2006). (Date of Access: 20.10.2023) (In Russ.).
6.URL: https://epp.genproc.gov.ru/web/proc_08/mass-media/news/archive?item=40848860. (In Russ.)
7.Boldurinova E. Uranium in Kalmykia: Legacy Sites and New Horizons. URL: https://tegrk.ru/archives/4598?ysclid=lj44arcobg815973413 (In Russ.)
8.URL: https://epp.genproc.gov.ru/web/proc_08/mass-media/news/archive?item=40848860. (Date of Access: 20.10.2023) (In Russ.).
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The work was financed under the State Contract as part of the Federal Target Program «Nuclear and Radiation Safety for 2016-2020 and for the period until 2030».
Contribution. Titov A.V. – data collection and processing, writing the text; Belskikh Iu.S. – data collection and processing, writing the text; Isaev D.V. – data collection and processing, writing the text; Shandala N.K. – study conception and design, writing and editing the text; Doroneva T.A. – sample measurements, statistical data processing; Bogdanov I.I. – sample measurements, statistical data processing; Semenova M.P. – literary material analysis, editing the text; Burtсev S.L. – sample measurements. All authors are responsible for approval of the final version of the article and integrity of all parts of the article.
Article received: 20.10.2023. Accepted for publication: 27.11.2023.