JOURNAL DESCRIPTION
The Medical Radiology and Radiation Safety journal ISSN 1024-6177 was founded in January 1956 (before December 30, 1993 it was entitled Medical Radiology, ISSN 0025-8334). In 2018, the journal received Online ISSN: 2618-9615 and was registered as an electronic online publication in Roskomnadzor on March 29, 2018. It publishes original research articles which cover questions of radiobiology, radiation medicine, radiation safety, radiation therapy, nuclear medicine and scientific reviews. In general the journal has more than 30 headings and it is of interest for specialists working in thefields of medicine¸ radiation biology, epidemiology, medical physics and technology. Since July 01, 2008 the journal has been published by State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency. The founder from 1956 to the present time is the Ministry of Health of the Russian Federation, and from 2008 to the present time is the Federal Medical Biological Agency.
Members of the editorial board are scientists specializing in the field of radiation biology and medicine, radiation protection, radiation epidemiology, radiation oncology, radiation diagnostics and therapy, nuclear medicine and medical physics. The editorial board consists of academicians (members of the Russian Academy of Science (RAS)), the full member of Academy of Medical Sciences of the Republic of Armenia, corresponding members of the RAS, Doctors of Medicine, professor, candidates and doctors of biological, physical mathematics and engineering sciences. The editorial board is constantly replenished by experts who work in the CIS and foreign countries.
Six issues of the journal are published per year, the volume is 13.5 conventional printed sheets, 88 printer’s sheets, 1.000 copies. The journal has an identical full-text electronic version, which, simultaneously with the printed version and color drawings, is posted on the sites of the Scientific Electronic Library (SEL) and the journal's website. The journal is distributed through the Rospechat Agency under the contract № 7407 of June 16, 2006, through individual buyers and commercial structures. The publication of articles is free.
The journal is included in the List of Russian Reviewed Scientific Journals of the Higher Attestation Commission. Since 2008 the journal has been available on the Internet and indexed in the RISC database which is placed on Web of Science. Since February 2nd, 2018, the journal "Medical Radiology and Radiation Safety" has been indexed in the SCOPUS abstract and citation database.
Brief electronic versions of the Journal have been publicly available since 2005 on the website of the Medical Radiology and Radiation Safety Journal: http://www.medradiol.ru. Since 2011, all issues of the journal as a whole are publicly available, and since 2016 - full-text versions of scientific articles. Since 2005, subscribers can purchase full versions of other articles of any issue only through the National Electronic Library. The editor of the Medical Radiology and Radiation Safety Journal in accordance with the National Electronic Library agreement has been providing the Library with all its production since 2005 until now.
The main working language of the journal is Russian, an additional language is English, which is used to write titles of articles, information about authors, annotations, key words, a list of literature.
Since 2017 the journal Medical Radiology and Radiation Safety has switched to digital identification of publications, assigning to each article the identifier of the digital object (DOI), which greatly accelerated the search for the location of the article on the Internet. In future it is planned to publish the English-language version of the journal Medical Radiology and Radiation Safety for its development. In order to obtain information about the publication activity of the journal in March 2015, a counter of readers' references to the materials posted on the site from 2005 to the present which is placed on the journal's website. During 2015 - 2016 years on average there were no more than 100-170 handlings per day. Publication of a number of articles, as well as electronic versions of profile monographs and collections in the public domain, dramatically increased the number of handlings to the journal's website to 500 - 800 per day, and the total number of visits to the site at the end of 2017 was more than 230.000.
The two-year impact factor of RISC, according to data for 2017, was 0.439, taking into account citation from all sources - 0.570, and the five-year impact factor of RISC - 0.352.
Issues journals
Medical Radiology and Radiation Safety. 2022. Vol. 67. № 2
Study of the Functional Relationship of the Brain in Patients
with Asymptomic Carotidal Senosis
Pospelova M.L., Efimtsev A.Y., Lepekhina A.S., Trufanov G.E., Alekseeva T.M., Ivanova N.E., Efimova M.Y., Voronin A.S.,
Makhanova A.M., Mikhalicheva A.A.
V.A. Almazov National Medical Research Centre, Sanct-Petersburgh, Russia
Contact person: A.S. Lepekhina, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To explore functional connectivity in patients with asymptomatic internal carotid arteries stenosis of more than 60% when using hirudotherapy.
Materials and methods: The analysis of the results of the examination of 15 patients aged 60 to 82 years with asymptomatic stenosis of the carotid arteries in the range of 60‒75 % was carried out. The patients underwent a course of hirudotherapy, consisting of 10 sessions. All patients underwent structural and functional magnetic resonance imaging with analysis of statistical data at rest, complaints and neurological status were assessed ‒ before and after the course of hirudotherapy.
Results: When using a course of hirudotherapy in patients with asymptomatic internal carotid arteries tenosis, when analyzing the neurological status and complaints, a significant improvement in well-being was noted. During functional magnetic resonance imaging, differences in functional connectivity between the medial prefrontal cortex and other areas of the brain were determined. Activation was noted in the main structures of the network of managerial control and identification of significance. Connectivity between the leading regions of the brain increased, which is a sign of improved brain activity.
Conclusion: Connectivity research can be used to study the functioning of brain networks and to determine the effectiveness of therapy. The course of hirudotherapy significantly changed the functional connectivity of the brain regions, the changes correlated with a decrease in the number of complaints. This work is a pilot and will be continued for the further development of treatment-and-prophylactic strategies with the inclusion of hirudotherapy in patients with a high risk of vascular accidents in a treatment.
Keywords: asymptomatic carotid stenosis, hirudotherapy, fMRI, connectome
For citation: Pospelova ML, Efimtsev AY, Lepekhina AS, Trufanov GE, Alekseeva TM, Ivanova NE, Efimova MY, Voronin AS, Makhanova AM, Mikhalicheva AA. Study of the functional relationship of the brain in patients with asymptomic carotidal senosis. Medical Radiology and Radiation Safety. 2022;67(2):59-63. (In Russian) doi: 10.33266/1024-6177-2022-67-2-59-63
References
1.Seung S. Konnektom = Connectome. Moscow, Binom. Laboratoriya Znaniy Publ., 2014. P. 440. ISBN 978-5-9963-1685-4
(In Russ.).
2.Fornito A., Bullmore E.T. Connectomics: a New Paradigm for Understanding Brain Disease. European Neuropsychopharmacology. 2015;25;5:733-748. DOI:10.1016/j.euroneuro.2014.02.011.
3.Fornito A., Zalesky A., Breakspear M. The Connectomics of Brain Disorders. Nature Reviews Neuroscience. 2015;16;3:159-172. DOI:10.1038/nrn3901.
4.Porshinsky B.S., Saha S., Grossman M.D., et al. Clinical Uses of the Medicinal Leech: a Practical Review. Journal of Postgraduate Medicine. 2011;57;1:65-71. DOI: 10.4103/0022-3859.74297.
5.Pospelova M.L., Barnaulov O.D. Doppler Study of the Effectiveness of Hirudotherapy in Patients with Chronic Vertebrobasilar Insufficiency and Stage 1 Discirculatory Encephalopathy. Regionarnoye Krovoobrashcheniye i Mikrotsirkulyatsiya = Regional Blood Circulation and Microcirculation. 2010;2;34:40-43. DOI:10.24884/1682-6655-2010-9-2-40-43 (In Russ.).
6.Semikova T.S., Semikova M.V. Girudoterapiya v Oftalmologicheskoy Praktike = Hirudotherapy in Ophthalmic Practice. Novyye Tekhnologii Mikrokhirurgii Glaza: Materialy XII Nauchno-Prakticheskoy Konferentsii = New Technologies of Eye Microsurgery: Materials of the XII Scientific-Practical Conference. Orenburg, IPK “Gazprompechat” Publ., 2001.
P. 65–66. ISBN 5-94397-017-7 (In Russ.).
7.Dobrynina L.A., Gadzhiyeva Z.SH., Morozova S.N., et al. Executive Functions: Fmri of Healthy Volunteers During Stroop Test and the Serial Count Test. Zhurnal Nevrologii i Psikhiatrii im. S.S. Korsakova. 2018;3:64-71. DOI:10.17116/jnevro201811811164 (In Russ.).
8.Pospelova M.L., Zaytsev D.Ye., Lepekhina A.S., YEfimtsev A.Yu., Alekseyeva T.M., Trufanov G.Ye. Cognitive Lesion in Patients with Asymptomic Carotid Stenoses more than 70% – Indication for Surgical Treatment? Sovremennyye Problemy Nauki i Obrazovaniya = Modern Problems of Science and Education. 2019;5. URL: http://www.science-education.ru/ru/article/view?id=29046 (Date of Access: 06/01/2021) (In Russ.).
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 30.11.2021. Accepted for publication: 30.03.2022.
Medical Radiology and Radiation Safety. 2022. Vol. 67. № 2
MEASUREMENT OF SOME CHEMICAL ELEMENTS IN NORMAL HUMAN BREAST TISSUE BY NEUTRON-ACTIVATION ANALYSIS
V. Zaichick, G.A. Davydov
A.F. Tsyb Medical Radiological Research Center, Obninsk, Russia
Contact person: V. Zaichick, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To develop the method of chemical element (ChE) content measurement in samples of breast tissue (BT) using the activation by neutrons of nuclear reactor combined with the high-resolution spectrometry of gamma-radiation of short-lived radionuclides (INAA-SLR), and to investigate ChE contents in normal human mammary gland.
Material and methods: In the samples of BT taken from female with intact mammary glands (mostly died from trauma, n = 38) the contents of calcium (Ca), chlorine (Cl), iodine (I), potassium (K), magnesium (Mg), manganese (Mn), sodium (Na), and strontium (Sr) were measured. To determine these element contents, the method of INAA-SLR was developed.
Results: The accuracy of the developed method and the reliability of the results obtained in the study were confirmed by the measurements of international certified reference material IAEA H-4 Animal Muscles and the good agreement with data of its certificate. The main statistic parameters, including, arithmetic mean, standard deviation, standard error of mean, minimum and maximum values, median, percentiles with 0.025 and 0.975 levels was calculated for ChE contents in the normal BT. It was shown, for example, that means of mass fractions ± standard errors of means (M±SEM, mg/kg dry tissue) in normal human mammary gland were: Ca 128±14, Cl 1014±146,
I 0,82±0,11, K 196±20, Mg 80,0±9,4, Mn 0,27±0,04, Na 665±71 и Sr 5,20±0,75. It was found that the Ca, Cl, I, K, Mg, Mn, Na и Sr contents in human mammary gland very differ from the normal level of these elements in whole blood, muscle and adipose tissue, particularly in contents of I and Sr.
Conclusion: The method of INAA-SLR allow to obtain the representative data on Ca, Cl, I, K, Mg, Mn, Na и Sr contents in human BT specimen. It was shown, that such trace elements as I and Sr may be directly involved in the mammary gland function.
Keywords: mammary gland, chemical elements of breast tissue, neutron-activation analysis
For citation: Zaichick V, Davydov GA. Measurement of some chemical elements in normal human breast tissue by neutron-activation analysis. Medical Radiology and Radiation Safety. 2022;67(2):64-68. (In Russian) doi: 10.33266/1024-6177-2022-67-2-64-68
References
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5. Dunchik V.N., Zherbin YE.A., Zaichick V.Ye., Leonov A.I., Sviridova T.V. Sposob Differentsialnoy Diagnostiki Zlokachestvennykh i Dobrokachestvennykh Opukholey Predstatelnoy Zhelezy = The Certificate for the Method of Differential Diagnosis of Malignant and Benign Prostate Tumors. № 764660 of the USSR. 1980 (In Russ.).
6. Zaichick V.Ye., Tsyb A.F., Dunchik V.N., Sviridova T.V. Sposob Diagnostiki Zabolevaniy Predstatelnoy Zhelezy = The Certificate for the Method of Diagnosis of Prostate Diseases.
№ 997281 of the USSR. Not Subject to Publication in the Open Press. 1982. (In Russ.).
7. Zaichick V.Ye., Tsyb A.F., Vtyurin B.M., Medvedev V.S. Sposob Diagnostiki Skrytogo Raka Shchitovidnoy Zhelezy = The Certificate for the Method of Diagnosis of Latent Thyroid Cancer. № 1096776 of the USSR. 1985 (In Russ.).
8. Shilstein S.Sh., Cortesi M., Breskin A., Chechik R., Vartsky D., Raviv G., Kleinman N., Ramon J., Kogan G., Gladysh V., Moriel E., Huszar M., Volkov A., Fridman E. Prostatic Zn Determination for Prostate Cancer Diagnosis. Talanta. 2006;70:914-921. D01:10.1016/j.talanta.2006.05.053.
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11. Costello L.C., Franklin R.B. Prostatic Fluid Electrolyte Composition for the Screening of Prostate Cancer: a Potential Solution to a Major Problem. Prostate Cancer Prostate Dis. 2009;12;1:17-24. DOI: 10.1038/pcan.2008.19.
12. Zaichick V. Medical Elementology as a New Scientific Discipline. J. Radioanal. Nucl. Chem. 2006;269;2:303-309. https://doi.org/10.1007/sl0967-006-0383-3.
13. Zaichick V. Applications of Synthetic Reference Materials in the Medical Radiological Research Centre. Fresenius J. Anal. Chem. 1995;352:219-223. https://doi.org/10.1007/BF00322330.
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DOI: 10.4236/jct.2017.82011.
15. Zaichick V. Sampling, Sample Storage and Preparation of Biomaterials for INAA in Clinical Medicine, Occupational and Environmental Health. Harmonization of Health-Related Environmental Measurements Using Nuclear and Isotopic Techniques. Vienna, IAEA, P. 123-133.
16. Zaichick V., Zaichick S. INAA Applied to Halogen (Br and I) Stability in Long-Term Storage of Lyophilized Biological Materials. J. Radioanal Nucl. Chem. 2000;244;2:279-281. hitps://doi.org/l 0.1023/A: 1006734114204.
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19. Kizalaite A., Brimiene V., Brimas G., Kiuberis J., Tautkus S., Zarkov A., Kareiva A. Determination of Trace Elements in Adipose Tissue of Obese People by Microwave-Assisted Digestion and Inductively Coupled Plasma Optical Emission Spectrometry. Biol. Trace. Elem. Res. 2019;189;1:10-17. DOI: 10.1007/sl2011-018-1450-7.
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 30.11.2021. Accepted for publication: 30.03.2022.
Medical Radiology and Radiation Safety. 2022. Vol. 67. № 2
NON-TRAUMATIC FORNIX RUPTURE WITH CONTRAST EXTRAVASATION
P.S. Kyzlasov, F.G. Kolpacynidi, D.V. Kazantsev, V.I. Doga, A.N. Bashkov, O.V. Parinov
Department of Urology and Andrology, Medical and Biological University of Innovation
and Continuing Education of A.I. Burnazyan Federal Medical Biophysical Center, Russia, Moscow
Contact person: P.S. Kyzlasov, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: Description of a clinical case of surgical treatment of nontraumatic rupture of the renal calyx fornix, with extravasation of contrast agent into the perirenal tissue.
Material and methods: Provided surgical treatment to a patient with spontaneous rupture of the calyx fornix on the background of ureteral obstruction caused by a stone.
Results: Considering the concomitant pathology in the form of obstructive pyelonephritis, it was decided to drain the upper urinary tract with an external stent. After 7 days, the patient underwent replacement of the external stent with the internal one. The patient underwent antibacterial, anti-inflammatory therapy, with a positive clinical, laboratory effect. Retrograde pyelography was performed 7 days later. Violations of the integrity of the cavity system of the left kidney were not revealed. The external stent was replaced with an internal one. After 2 months, ureterolitho-extraction was performed on the left.
Conclusion: From the data of the world literature and our own experience, we can conclude that spontaneous, non-traumatic rupture of the fornix is an extremely rare, urgent urological disease. We assume that the development of this process is similar to the Burhave syndrome, since smooth muscle fibers are present in the esophagus as well as in the esophagus. In order to provide immediate assistance to such patients, it is necessary to urgently drain the upper urinary tract. Timely diagnosis of this condition requires rapid tactics in the form of immediate ultrasound, radiological diagnostic methods, as well as computed tomography. The operational manual on the removal of stones in this case should be postponed for 2–3 weeks.
Keywords: rupture of the renal cup, spontaneous rupture of the calyx, kidney injury, urinary extravasation, urinoma, kidney stones
For citation: Kyzlasov PS, Kolpacynidi FG, Kazantsev DV, Doga VI, Bashkov AN, Parinov OV. Non-traumatic fornix rupture with contrast extravasation. Medical Radiology and Radiation Safety. 2022;67(2):73-75. (In Russian) doi: 10.33266/1024-6177-2022-67-2-73-75
References
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2. Daliakopoulos S.I. Spontaneous Retroperitoneal Hematoma: a Rare Devastating Clinical Entity of a Pleiada of Less Common Origins. J. Surg. Tech. Case. Rep. 2011;3;1:8-9. doi:10.4103/2006-8808.78462.
3. Aliabadi H.A., Cass A.S., Ireland G.W., Matsuura J.K. Spontaneous Rupture of Hydronephrotic Renal Pelvis with Massive Hemorrhage. Urology. 1985;25;1:17-21. doi:10.1016/0090-4295(85)90555-2.
4. Miranda R., Latorre P., Raurich R. Bilateral Spontaneous Renal Pelvis Rupture During a Computed Tomography: Report of One Case. Rev. Med. Chil. 2017;145;4:544-548. doi:10.4067/S0034-98872017000400016. (In Spanish). [Miranda R., Latorre P., Raurich R. Ruptura Pieloureteral Espontánea Bilateral Durante un Urotac: Caso Clínico // Rev. Med. Chil. 2017. V.145, No. 4. P. 544-548. doi:10.4067/S0034-98872017000400016].
5. Sayeed R., Nyamekye I., Kinder R. Unsuspected Rectal Adenocarcinoma Causing a Urinoma. Int. J. Urol. 1997;4;1:99-100. doi:10.1111/j.1442-2042.1997.tb00151.x.
6. Chen T., Su Y., Tang L., Chang W., Chen C. Spontaneous Rupture of Renal Pelvis. International Journal of Gerontology. 2007;1;3:131-133.
7. Morgan T.N., Bandari J., Shahait M., Averch T.. Renal Forniceal Rupture: Is Conservative Management Safe? Urology. 2017;109:51-54. doi:10.1016/j.urology.2017.07.045.
8. Pytel A.Ya., Pytel Yu.A. X-ray Diagnostics of Urological Diseases. Moscow Publ., 2003. P. 18-19 (In Russ.). [Пытель А.Я.,
Пытель Ю.А. Рентгенодиагностика урологических забо-
леваний. Москва, 2003. 18-19].
PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 30.11.2021. Accepted for publication: 30.03.2022.
Medical Radiology and Radiation Safety. 2022. Vol. 67. № 2
Study of the Influence of the Layer Thickness
of Composite Coatings Based on Barium Sulphate
on the Attenuation of X-Ray Radiation
O.V. Boiprav1, N.N. Grinchik2, V.D. Guzov3, D.I. Penialosa Ovalies1
1Belarusian State University of Informatics and Radioelectronics, Minsk, Republic of Belarus
2 A.V. Lykov Institute of Heat and Mass Transfer, Minsk, Republic of Belarus
3Scientific and Production Unitary Enterprise ATOMTECH JSC MNIPI, Minsk, Republic of Belarus
Contact person: O.V. Boiprav, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: Defining the regularities in X-ray radiation attenuation, which characterize composite coatings based on barium sulfate and the aqueous alkaline sodium silicate solution, depending on the thickness of the layer of such coatings deposition.
Material and methods: The experiment consisted in the manufacture of experimental samples of composite coatings based on barium sulfate and the aqueous alkaline solution of sodium silicate and testing of the manufactured experimental samples. Tests of the manufactured experimental samples of composite coatings were carried out using UPR-AT300 calibration X-ray unit, DKS-AT5350 precision dosimeter in combination with a TM23361 ionization chamber (PTW Freiburg), attorney at Mendeleev All-Russian Research Institute of Metrology (Russia) on the state standards of the Russian Federation.
Results: It has been established that by increasing from 0.5 mm to 2.0 mm of the thickness of the deposition layer of these coatings, it is possible to ensure an increase of 2.0‒3.5 times of X-ray attenuation ratio values in the range of average energies 30.0‒60,0 keV.
Conclusion: The obtained results are recommended for use in solving problems related to the shielding of premises (X-ray rooms) or areas of premises (inspection zones at airports) in which X-ray sources are located.
Keywords: attenuation coefficient, X-ray radiation, composite coating, barium sulfate
For citation: Boiprav OV, Grinchik NN, Guzov VD, Penialosa Ovalies DI.Study of the Influence of the Layer Thickness of Composite Coatings Based on Barium Sulphate on the Attenuation of X-Ray Radiation. Medical Radiology and Radiation Safety. 2022;67(2):69-72. (In Russian) doi: 10.33266/1024-6177-2022-67-2-69-72
References
1. Tada Katsumi, Yamakata Koudzi, Yokoyama Itaru, Katsumoto Kodzi. Kompozitsiya dlya Stroitelnogo Materiala i Gipsovaya Plita, a takzhe Sposob Stroitelstva s ikh Ispolzovaniyem i Stena = Composition for Building Material and Gypsum Board, as Well as a Method of Construction Using them and a Wall. Patent of the Russian Federation No. RU2393563C2. Publ. 27.06.2010 (In Russian).
2. Gröbner Gosbert, Vogel Petra, Kremer Georg, Scheller Lothar, Hummel Hans-Ulrich. Sposob Polucheniya Stroitelnoy Plity na Osnove Sulfata Kaltsiya/Sulfata Bariya = A Method of Producing a Building Board Based on Calcium Sulfate/Barium Sulfate. Patent of the Russian Federation No. RU 2440314. Publ. 20.01.2012 (In Russian).
3. Bulat A.F., Ivanov V.A., Zvyagilskiy E.L., Golov K.S., Bolshakov V.I. Comparative studies of radiation-protective fillers of composite materials based on phosphogypsum binder. Vostochno-Yevropeyskiy Zhurnal Peredovykh Tekhnologiy = Eastern European Journal of Advanced Technologies. 2017;6/11;78:25-29. DOI: 10.15587 / 1729-4061.2015.54902 (In Russian).
4. McNaught A.D., Wilkinson A. Standard Conditions for Gases. IUPAC. Compendium of Chemical Terminology. Oxford, 1997.
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PDF (RUS) Full-text article (in Russian)
Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 30.11.2021. Accepted for publication: 30.03.2022.
Medical Radiology and Radiation Safety. 2022. Vol. 67. № 2
Combined Use of Surgical Excision of Radiation Ulcers and
Transplantation Syngeneic Cells of the Stromal-Vascular
Fraction of Adipose Tissue in the Treatmentof
Severe Local Radiation Lesions in the Experiments
Yu. B. Deshevoi, V. G. Lebedev, T.A. Nasonova, O.A. Dobrynina, A.V. Lyrshhikova, T.A. Astrelina, B.B. Moroz
A.I. Burnasyan Federal Medical Biophysical Center, Moscow, Russia
Contact person: Yu.B. Deshevoi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
Purpose: To study the therapeutic efficacy of the combined use of surgical excision of radiation ulcers and transplantation of syngeneic cells of the stromal-vascular fraction (SVF) of adipose tissue in the treatment of severe local radiation lesions.
Material and methods: Experiments were conducted on rats males of the inbred line Wista‒Kyoto weighing 230‒250 g. Animals were irradiated on an X-ray unit locally in the iliac-lumbar region of the back at a dose of 110 Gy (voltage on the tube 30 kV, current strength
6.1 mA, filter 0.1 mm Al), at a dose rate of 20,0 Gy/min. Irradiation field area 8.2 cm2. Radiation exposure made it possible to obtain severe skin lesions in rats with long-term non-healing ulcers, and without critical radiation exposure to the underlying tissues. Morphological study showed that the area of skin necrosis (with a maximum in the center of the irradiation zone) was formed by 21‒23 days after irradiation and was characterized by the presence of pronounced changes in all layers of the skin. Surgical excision of radiation ulcers was performed
on 22 days after exposure to radiation. The affected tissue was removed to the entire depth of the skin up to the fascia of skeletal muscles. Excision of the skin was carried out, retreating 6‒8 mm from the outer border of the radiation ulcer. Cell transplantation was carried out twice 5 and 12 days after excision of the ulcer. SVF cells were obtained from intact animals from subcutaneous adipose tissue treated with trypsin. A suspension of SVF cells of adipose tissue in 1 ml of a sterile Solution of Henks was injected under the skin at 5 points around the excision zone, retreating 5‒7 mm from the edge of the focus. The number of injected cells in one transplant was 2.5×106.
Results: The area of radiation ulcers in rats of the control group in the period from 27 to 105 days after irradiation slowly decreased from 2.8±0.2 cm2 to 1.2 ± 0.2 cm2. Excision of radiation ulcers led to rapid complete visible healing of the wound defect (already by 70 days after irradiation) with the formation of atrophic scars. Transplantation of SVF syngeneic cells increased the rate of healing of the surgical wound.
Conclusion: The results show that surgical excision of radiation ulcers in combination with cell therapy may be an effective way to treat severe local radiation lesions.
Keywords: radiation ulcers, surgical excision, transplantation, stromal-vascular fraction of adipose tissue, rats
For citation: Deshevoi YuB, Lebedev VG, Nasonova TA, Dobrynina OA, LyrshhikovaAV, Astrelina TA, Moroz BB. Combined use of surgical excision of radiation ulcers and transplantation syngeneic cells of the stromal-vascular fraction (svf) of adipose tissue in the treatment
of severe local radiation lesions in the experiments. Medical Radiology and Radiation Safety. 2022;67(2):5-9. (In Russian)
doi: 10.33266/1024-6177-2022-67-2-5-9
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Conflict of interest. The authors declare no conflict of interest.
Financing. The study had no sponsorship.
Contribution. Article was prepared with equal participation of the authors.
Article received: 30.11.2021. Accepted for publication: 30.03.2022.